DAPK3 ELISA Kit for Human(Homo sapiens) | EIAab.comDAPK3 ELISA Kit for Human(Homo sapiens)

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DAPK3 (Gene name),

Death-associated protein kinase 3 (Protein name ), DAPK3_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

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Gene name:

DAPK3(ZIPK);

Protein name:

Death-associated protein kinase 3(DAP kinase 3);

Alternative:

ZIP-kinase;DAP-like kinase(Dlk);Zipper-interacting protein kinase(ZIP-kinase);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homodimer or forms heterodimers with ATF4. Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. Also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. Interacts with AATF, CDC5L, UBE2D1, UBE2D2 AND UBE2D3.

Function:

Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.

Subcellular Location:

Nucleus Cytoplasm Nucleus PML body Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Localizes to promyelocytic leukemia protein nuclear bodies (PML-NBs). Associates to centromeres from prophase to anaphase.

Protein Attributes:

Sequence length:

454

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

454:

CGLR

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

hsa:1613

SMR:

O43293

STRING:

9606.ENSP00000301264

String:

O43293

Pfam:

PF00069

UniGene:

Hs.631844

MIM:

603289

Uniprot:

O43293

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ELISA Kit for Human DAP kinase 3

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E15322m

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96T

ELISA Kit for Human DAP kinase 3

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E15322r

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96T

ELISA Kit for Human DAP kinase 3

Cat.:

E15322h

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96T

CLIA Kit for Human DAP kinase 3

Cat.:

U15322h

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96T

CLIA Kit for Human DAP kinase 3

Cat.:

U15322r

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96T

CLIA Kit for Human DAP kinase 3

Cat.:

U15322m

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96T

Polyclonal Antibody for Human DAP kinase 3

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P15322Rb-m

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40ug/0.2ml

Polyclonal Antibody for Human DAP kinase 3

Cat.:

P15322Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human DAP kinase 3

Cat.:

P15322Rb-h

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40ug/0.2ml

Monoclonal Antibody for Human DAP kinase 3

Protein for Human DAP kinase 3

R&D Technical Data

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Precision

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Recovery

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Linearity

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References

1.

"ZIP kinase, a novel serine/threonine kinase which mediates apoptosis."
Kawai T. , Matsumoto M. , Takeda K. , Sanjo H. , Akira S.
Mol. Cell. Biol.18:1642-1651(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
2.

"ZIP kinase identified as a novel myosin regulatory light chain kinase in HeLa cells."
Murata-Hori M. , Suizu F. , Iwasaki T. , Kikuchi A. , Hosoya H.
FEBS Lett.451:81-84(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];FUNCTION;CATALYTIC ACTIVITY;COFACTOR
tissue: Cervix carcinoma.
3.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
4.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Brain.
5.

"Novel ZIP kinase isoform lacks leucine zipper."
Takamoto N. , Komatsu S. , Komaba S. , Niiro N. , Ikebe M.
Arch. Biochem. Biophys.456:194-203(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 165-454 (ISOFORM 2);ALTERNATIVE SPLICING;FUNCTION IN PHOSPHORYLATION OF MYOSIN; PPP1R12A AND MYL12B;CATALYTIC ACTIVITY;SUBCELLULAR LOCATION;BIOPHYSICOCHEMICAL PROPERTIES;INTERACTION WITH PPP1R12A AND MYOSIN;AUTOPHOSPHORYLATION;TISSUE SPECIFICITY
tissue: Urinary bladder.
6.

"Zipper-interacting protein kinase induces Ca(2+)-free smooth muscle contraction via myosin light chain phosphorylation."
Niiro N. , Ikebe M.
J. Biol. Chem.276:29567-29574(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF MUSCLE MYL12B;CATALYTIC ACTIVITY
7.

"HeLa ZIP kinase induces diphosphorylation of myosin II regulatory light chain and reorganization of actin filaments in nonmuscle cells."
Murata-Hori M. , Fukuta Y. , Ueda K. , Iwasaki T. , Hosoya H.
Oncogene20:8175-8183(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF NON-MUSCLE MYL12B;CATALYTIC ACTIVITY
8.

"ZIP kinase triggers apoptosis from nuclear PML oncogenic domains."
Kawai T. , Akira S. , Reed J.C.
Mol. Cell. Biol.23:6174-6186(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN APOPTOSIS;INTERACTION WITH DAXX AND PAWR
9.

"Identification and characterization of zipper-interacting protein kinase as the unique vascular smooth muscle myosin phosphatase-associated kinase."
Endo A. , Surks H.K. , Mochizuki S. , Mochizuki N. , Mendelsohn M.E.
J. Biol. Chem.279:42055-42061(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PPP1R12A;TISSUE SPECIFICITY
10.

"ZIP kinase is responsible for the phosphorylation of myosin II and necessary for cell motility in mammalian fibroblasts."
Komatsu S. , Ikebe M.
J. Cell Biol.165:243-254(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF MYL12B;CATALYTIC ACTIVITY
11.

"Death-associated protein kinase phosphorylates ZIP kinase, forming a unique kinase hierarchy to activate its cell death functions."
Shani G. , Marash L. , Gozuacik D. , Bialik S. , Teitelbaum L. , Shohat G. , Kimchi A.
Mol. Cell. Biol.24:8611-8626(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;PHOSPHORYLATION AT THR-299; SER-309; SER-311; SER-312; SER-318 AND SER-326;PHOSPHORYLATION BY DAPK1;SUBCELLULAR LOCATION
12.

"Physical and functional interactions between STAT3 and ZIP kinase."
Sato N. , Kawai T. , Sugiyama K. , Muromoto R. , Imoto S. , Sekine Y. , Ishida M. , Akira S. , Matsuda T.
Int. Immunol.17:1543-1552(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH STAT3
13.

"TCP10L is expressed specifically in spermatogenic cells and binds to death associated protein kinase-3."
Yu H. , Jiang D. , Guo Z. , Saiyin H. , Guo J. , Wang X. , Yu L.
Int. J. Androl.28:163-170(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TCP10L;SUBCELLULAR LOCATION
14.

"Regulation of zipper-interacting protein kinase activity in vitro and in vivo by multisite phosphorylation."
Graves P.R. , Winkfield K.M. , Haystead T.A.
J. Biol. Chem.280:9363-9374(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-180; THR-225; THR-265; THR-299; THR-306 AND SER-311;ENZYME REGULATION;SUBCELLULAR LOCATION;MUTAGENESIS OF THR-299; 299-THR-THR-300; VAL-427; VAL-434 AND LEU-441
15.

"The death-associated protein kinases: structure, function, and beyond."
Bialik S. , Kimchi A.
Annu. Rev. Biochem.75:189-210(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON FUNCTION
16.

"ROCK1 phosphorylates and activates zipper-interacting protein kinase."
Hagerty L. , Weitzel D.H. , Chambers J. , Fortner C.N. , Brush M.H. , Loiselle D. , Hosoya H. , Haystead T.A.
J. Biol. Chem.282:4884-4893(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;ENZYME REGULATION;AUTOPHOSPHORYLATION;PHOSPHORYLATION AT THR-180; THR-265 AND THR-299;MUTAGENESIS OF LYS-42; ASP-161; THR-225; THR-265 AND THR-299
17.

"ZIPK: a unique case of murine-specific divergence of a conserved vertebrate gene."
Shoval Y. , Pietrokovski S. , Kimchi A.
PLoS Genet.3:1884-1893(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;ABSENCE OF INTERACTION WITH PARW
18.

"Dlk/ZIP kinase, a novel Ser/Thr-specific protein kinase with multiple functions."
Scheidtmann K.H.
Signal Transduct.7:248-259(2007)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON FUNCTION
19.

"DAPK-ZIPK-L13a axis constitutes a negative-feedback module regulating inflammatory gene expression."
Mukhopadhyay R. , Ray P.S. , Arif A. , Brady A.K. , Kinter M. , Fox P.L.
Mol. Cell32:371-382(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHPRYLATION OF RPL13A;CATALYTIC ACTIVITY
20.

"ZIP kinase plays a crucial role in androgen receptor-mediated transcription."
Leister P. , Felten A. , Chasan A.I. , Scheidtmann K.H.
Oncogene27:3292-3300(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN ANDROGEN RECEPTOR-MEDIATED TRANSCRIPTION
21.

"Cancer-associated loss-of-function mutations implicate DAPK3 as a tumor-suppressing kinase."
Brognard J. , Zhang Y.W. , Puto L.A. , Hunter T.
Cancer Res.71:3152-3161(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION AS TUMOR SUPPRESSOR;CHARACTERIZATION OF VARIANTS MET-112; ASN-161 AND SER-216;MUTAGENESIS OF THR-180;SELF-ASSOCIATION
22.

"Phosphorylation-dependent control of ZIPK nuclear import is species specific."
Weitzel D.H. , Chambers J. , Haystead T.A.
Cell. Signal.23:297-303(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-299;SUBCELLULAR LOCATION;MUTAGENESIS OF 294-ARG-ARG-295
23.

"Atg1-mediated myosin II activation regulates autophagosome formation during starvation-induced autophagy."
Tang H.W. , Wang Y.B. , Wang S.L. , Wu M.H. , Lin S.Y. , Chen G.C.
EMBO J.30:636-651(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN REGULATION OF AUTOPHAGY;INTERACTION WITH ULK1
24.

"Zipper-interacting protein kinase (ZIPK) modulates canonical Wnt/beta-catenin signaling through interaction with Nemo-like kinase and T-cell factor 4 (NLK/TCF4)."
Togi S. , Ikeda O. , Kamitani S. , Nakasuji M. , Sekine Y. , Muromoto R. , Nanbo A. , Oritani K. , Kawai T. , Akira S. , Matsuda T.
J. Biol. Chem.286:19170-19177(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH NLK
25.

"New modularity of DAP-kinases: alternative splicing of the DRP-1 gene produces a ZIPk-like isoform."
Shoval Y. , Berissi H. , Kimchi A. , Pietrokovski S.
PLoS ONE6:E17344-E17344(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH ATF4
26.

"Interaction of RhoD and ZIP kinase modulates actin filament assembly and focal adhesion dynamics."
Nehru V. , Almeida F.N. , Aspenstrom P.
Biochem. Biophys. Res. Commun.433:163-169(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN REORGANIZATION OF ACTIN CYTOSKELETON;INTERACTION WITH RHOD
27.

"Crystal structure of human ZIP kinase."
Kursula P. , Vahokoski J. , Wilmanns M.
Submitted (2006-06) to the PDB data bank

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 1-277
28.

"Activation segment dimerization: a mechanism for kinase autophosphorylation of non-consensus sites."
Pike A.C. , Rellos P. , Niesen F.H. , Turnbull A. , Oliver A.W. , Parker S.A. , Turk B.E. , Pearl L.H. , Knapp S.
EMBO J.27:704-714(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 9-289 IN COMPLEX WITH PYRIDONE 6;ENZYME REGULATION;SUBUNIT;PHOSPHORYLATION AT SER-50 AND THR-265
29.

"Patterns of somatic mutation in human cancer genomes."
Greenman C. , Stephens P. , Smith R. , Dalgliesh G.L. , Hunter C. , Bignell G. , Davies H. , Teague J. , Butler A. , Stevens C. , Edkins S. , O'Meara S. , Vastrik I. , Schmidt E.E. , Avis T. , Barthorpe S. , Bhamra G. , Buck G. , more...
Nature446:153-158(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS [LARGE SCALE ANALYSIS] MET-112; ASN-161 AND SER-216

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