Protein name:
H/ACA ribonucleoprotein complex subunit 4 ;
Alternative:
Nopp140-associated protein of 57 kDa ;Dyskerin ;Nucleolar protein family A member 4 ;Nucleolar protein NAP57 ;snoRNP protein DKC1 ;
Organism:
Mouse (Mus musculus).
General Annotation
Sub Unit:
Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which contains NHP2/NOLA2, GAR1/NOLA1, NOP10/NOLA3, and DKC1/NOLA4, which is presumed to be the catalytic subunit. The complex contains a stable core formed by binding of one or two NOP10-DKC1 heterodimers to NHP2; GAR1 subsequently binds to this core via DKC1. The complex binds a box H/ACA small nucleolar RNA (snoRNA), which may target the specific site of modification within the RNA substrate. During assembly, the complex contains NAF1 instead of GAR1/NOLA1. The complex also interacts with TERC, which contains a 3'-terminal domain related to the box H/ACA snoRNAs. Specific interactions with snoRNAs or TERC are mediated by GAR1 and NHP2. Associates with NOLC1/NOPP140. H/ACA snoRNPs interact with the SMN complex, consisting of SMN1 or SMN2, GEMIN2/SIP1, DDX20/GEMIN3, and GEMIN4. This is mediated by interaction between GAR1 and SMN1 or SMN2. The SMN complex may be required for correct assembly of the H/ACA snoRNP complex. Component of the telomerase holoenzyme complex at least composed of TERT, DKC1, WRAP53/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and a telomerase RNA template component (TERC) (By similarity). Interacts with SHQ1; this interaction may lead to the stabilization of DKC1, from the time of its synthesis until its association with NOP10, NHP2, and NAF1 at the nascent H/ACA RNA.
Function:
Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
Subcellular Location:
Nucleus
nucleolus
Nucleus
Cajal body
Also localized to Cajal bodies.
Protein Attributes:
50:
MADAEVITFP | KKHKKKKDRK | PLQEDDVAEI | QHAEEFLIKP | ESKVAQLDTS |
100:
QWPLLLKNFD | KLNVRTAHYT | PLPCGSNPLK | REIGDYIRTG | FINLDKPSNP |
150:
SSHEVVAWIR | RILRVEKTGH | SGTLDPKVTG | CLIVCIERAT | RLVKSQQSAG |
200:
KEYVGIVRLH | NAIEGGTQLS | RALETLTGAL | FQRPPLIAAV | KRQLRVRTIY |
250:
ESKMIEYDPE | RRLGIFWVSC | EAGTYIRTLC | VHLGLLLGVG | GQMQELRRVR |
300:
SGVMSEKDHM | VTMHDVLDAQ | WLYDNHKDES | YLRRVVYPLE | KLLTSHKRLV |
350:
MKDSAVNAIC | YGAKIMLPGL | LRYEDGIEVN | QEIVVITTKG | EAICMAIALM |
400:
TTAVISTCDH | GIVAKIKRVI | MERDTYPRKW | GLGPKASQKK | MMIKQGLLDK |
450:
HGKPTDNTPA | TWKQDYIDYS | DSGKNTLVTE | AVQAPQLAAE | AVNVIKRKRD |
500:
SESESDETPT | VPQLKEKKKK | KDKKPKTVLE | SGGETGDGDN | DTTKKKKKKK |
Vaild Sequence:
Related Databases
Uniprot:
ELISA Kit
CLIA Kit
Polyclonal Antibody
Monoclonal Antibody
Protein
FOR
Chicken
Human
Rat
Mouse
ELISA Kit for Mouse H/ACA ribonucleoprotein complex subunit 4
ELISA Kit for Mouse H/ACA ribonucleoprotein complex subunit 4
ELISA Kit for Mouse H/ACA ribonucleoprotein complex subunit 4
ELISA Kit for Mouse H/ACA ribonucleoprotein complex subunit 4
CLIA Kit for Mouse H/ACA ribonucleoprotein complex subunit 4
CLIA Kit for Mouse H/ACA ribonucleoprotein complex subunit 4
CLIA Kit for Mouse H/ACA ribonucleoprotein complex subunit 4
CLIA Kit for Mouse H/ACA ribonucleoprotein complex subunit 4
Polyclonal Antibody for Mouse H/ACA ribonucleoprotein complex subunit 4
Polyclonal Antibody for Mouse H/ACA ribonucleoprotein complex subunit 4
Polyclonal Antibody for Mouse H/ACA ribonucleoprotein complex subunit 4
Polyclonal Antibody for Mouse H/ACA ribonucleoprotein complex subunit 4
Monoclonal Antibody for Mouse H/ACA ribonucleoprotein complex subunit 4
Monoclonal Antibody for Mouse H/ACA ribonucleoprotein complex subunit 4
Monoclonal Antibody for Mouse H/ACA ribonucleoprotein complex subunit 4
Monoclonal Antibody for Mouse H/ACA ribonucleoprotein complex subunit 4
Protein for Mouse H/ACA ribonucleoprotein complex subunit 4
Protein for Mouse H/ACA ribonucleoprotein complex subunit 4
Protein for Mouse H/ACA ribonucleoprotein complex subunit 4
Protein for Mouse H/ACA ribonucleoprotein complex subunit 4
R&D Technical Data
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Precision
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Recovery
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Linearity
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
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129/Ola .
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Europe PMC ]
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Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain :
C57BL/6J .
tissue :
Colon .
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Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
strain :
C57BL/6J .
4.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION
5.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION;MUTAGENESIS OF ALA-353 AND GLY-402
6.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-508;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
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Brain .
7.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-451; SER-453 AND SER-481;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Liver .
8.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-451; SER-453 AND SER-455;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Liver .
9.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-508;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
10.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-451; SER-453; SER-455 AND SER-508;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Embryonic fibroblast .