Dnmt3a ELISA Kit for Mouse(Mus musculus) | EIAab.comDnmt3a ELISA Kit for Mouse(Mus musculus)

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Dnmt3a (Gene name),

DNA (cytosine-5)-methyltransferase 3A (Protein name ), DNM3A_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Dnmt3a;

Protein name:

DNA (cytosine-5)-methyltransferase 3A(Dnmt3a);

Alternative:

DNA methyltransferase MmuIIIA(DNA MTase MmuIIIA;M.MmuIIIA);

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Heterotetramer composed of 1 DNMT3A homodimer and 2 DNMT3L subunits (DNMT3L-DNMT3A-DNMT3A-DNMT3L). Interacts with DNMT1 and DNMT3B (By similarity). Binds the ZNF238 transcriptional repressor. Interacts with SETDB1. Associates with HDAC1 through its ADD domain. Interacts with the PRC2/EED-EZH2 complex. Interacts with UBC9, PIAS1 and PIAS2.

Function:

Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.

Subcellular Location:

Nucleus Cytoplasm Accumulates in the major satellite repeats at pericentric heterochromatin.

Protein Attributes:

Sequence length:

908

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

750:

800:

850:

900:

908:

LKEYFACV

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

mmu:13435

UniGene:

Mm.5001

String:

O88508

SMR:

O88508

UniGene:

Mm.470389

Pfam:

PF00145

Uniprot:

O88508

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ELISA Kit for Mouse Dnmt3a

Cat.:

E13889h

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96T

ELISA Kit for Mouse Dnmt3a

Cat.:

E13889r

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96T

ELISA Kit for Mouse Dnmt3a

Cat.:

E13889m

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96T

ELISA Kit for Mouse Dnmt3a

Cat.:

E13889c

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96T

CLIA Kit for Mouse Dnmt3a

Cat.:

U13889m

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MSDS:

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96T

CLIA Kit for Mouse Dnmt3a

Cat.:

U13889r

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Packing:

96T

CLIA Kit for Mouse Dnmt3a

Cat.:

U13889h

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96T

CLIA Kit for Mouse Dnmt3a

Cat.:

U13889c

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96T

Polyclonal Antibody for Mouse Dnmt3a

Cat.:

P13889Rb-r

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40ug/0.2ml

Polyclonal Antibody for Mouse Dnmt3a

Cat.:

P13889Rb-h

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40ug/0.2ml

Polyclonal Antibody for Mouse Dnmt3a

Cat.:

P13889Rb-m

Price:

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40ug/0.2ml

Monoclonal Antibody for Mouse Dnmt3a

Protein for Mouse Dnmt3a

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

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References

1.

"Cloning and characterization of a family of novel mammalian DNA (cytosine-5) methyltransferases."
Okano M. , Xie S. , Li E.
Nat. Genet.19:219-220(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
2.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
tissue: Mammary gland.
3.

"The transcriptional landscape of the mammalian genome."
Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2)
strain: C57BL/6.
strain: C57BL/6J.
tissue: Brain.
tissue: Embryo.
tissue: Skin.
4.

"A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation."
Chen T. , Ueda Y. , Xie S. , Li E.
J. Biol. Chem.277:38746-38754(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2);SUBCELLULAR LOCATION;ALTERNATIVE PROMOTER USAGE;TISSUE SPECIFICITY
strain: 129/SvJ.
5.

"DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development."
Okano M. , Bell D.W. , Haber D.A. , Li E.
Cell99:247-257(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;DEVELOPMENTAL STAGE
6.

"Enzymatic properties of recombinant Dnmt3a DNA methyltransferase from mouse: the enzyme modifies DNA in a non-processive manner and also methylates non-CpG correction sites."
Gowher H. , Jeltsch A.
J. Mol. Biol.309:1201-1208(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
7.

Gowher H. , Jeltsch A.
J. Mol. Biol.310:951-951(2001)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM
8.

"Dnmt3a binds deacetylases and is recruited by a sequence-specific repressor to silence transcription."
Fuks F. , Burgers W.A. , Godin N. , Kasai M. , Kouzarides T.
EMBO J.20:2536-2544(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH ZBTB18 AND HDAC1
9.

"Molecular enzymology of the catalytic domains of the Dnmt3a and Dnmt3b DNA methyltransferases."
Gowher H. , Jeltsch A.
J. Biol. Chem.277:20409-20414(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
10.

"Biochemical fractionation reveals association of DNA methyltransferase (Dnmt) 3b with Dnmt1 and that of Dnmt 3a with a histone H3 methyltransferase and Hdac1."
Datta J. , Ghoshal K. , Sharma S.M. , Tajima S. , Jacob S.T.
J. Cell. Biochem.88:855-864(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION IN A COMPLEX WITH HDAC1
11.

"The PWWP domain of Dnmt3a and Dnmt3b is required for directing DNA methylation to the major satellite repeats at pericentric heterochromatin."
Chen T. , Tsujimoto N. , Li E.
Mol. Cell. Biol.24:9048-9058(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;MUTAGENESIS OF 302-VAL-PRO-303 AND 705-PRO-CYS-706
12.

"Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting."
Kaneda M. , Okano M. , Hata K. , Sado T. , Tsujimoto N. , Li E. , Sasaki H.
Nature429:900-903(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
13.

"Modification of de novo DNA methyltransferase 3a (Dnmt3a) by SUMO-1 modulates its interaction with histone deacetylases (HDACs) and its capacity to repress transcription."
Ling Y. , Sankpal U.T. , Robertson A.K. , McNally J.G. , Karpova T. , Robertson K.D.
Nucleic Acids Res.32:598-610(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUMOYLATION;INTERACTION WITH UBC9; PIAS1 AND PIAS2
14.

"Mechanism of stimulation of catalytic activity of Dnmt3A and Dnmt3B DNA-(cytosine-C5)-methyltransferases by Dnmt3L."
Gowher H. , Liebert K. , Hermann A. , Xu G. , Jeltsch A.
J. Biol. Chem.280:13341-13348(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ENZYME REGULATION
15.

"Distinct DNA methylation activity of Dnmt3a and Dnmt3b towards naked and nucleosomal DNA."
Takeshima H. , Suetake I. , Shimahara H. , Ura K. , Tate S. , Tajima S.
J. Biochem.139:503-515(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
16.

"Mutational analysis of the catalytic domain of the murine Dnmt3a DNA-(cytosine C5)-methyltransferase."
Gowher H. , Loutchanwoot P. , Vorobjeva O. , Handa V. , Jurkowska R.Z. , Jurkowski T.P. , Jeltsch A.
J. Mol. Biol.357:928-941(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF PHE-636; GLU-660; ASP-682; CYS-706; ASN-707; SER-710; ARG-716; LYS-717; GLU-752; ASN-753; ARG-788; ARG-827; ARG-832; ARG-878; ARG-881 AND ARG-883
17.

"The Polycomb group protein EZH2 directly controls DNA methylation."
Vire E. , Brenner C. , Deplus R. , Blanchon L. , Fraga M. , Didelot C. , Morey L. , Van Eynde A. , Bernard D. , Vanderwinden J.-M. , Bollen M. , Esteller M. , Di Croce L. , de Launoit Y. , Fuks F.
Nature439:871-874(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH THE PRC2/EED-EZH2 COMPLEX
18.

Vire E. , Brenner C. , Deplus R. , Blanchon L. , Fraga M. , Didelot C. , Morey L. , Van Eynde A. , Bernard D. , Vanderwinden J.-M. , Bollen M. , Esteller M. , Di Croce L. , de Launoit Y. , Fuks F.
Nature446:824-824(2006)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM
19.

"Structure of Dnmt3a bound to Dnmt3L suggests a model for de novo DNA methylation."
Jia D. , Jurkowska R.Z. , Zhang X. , Jeltsch A. , Cheng X.
Nature449:248-251(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;ENZYME REGULATION;MUTAGENESIS OF PHE-728
20.

"Mouse Dnmt3a preferentially methylates linker DNA and is inhibited by histone H1."
Takeshima H. , Suetake I. , Tajima S.
J. Mol. Biol.383:810-821(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
21.

"Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells."
Meilinger D. , Fellinger K. , Bultmann S. , Rothbauer U. , Bonapace I.M. , Klinkert W.E. , Spada F. , Leonhardt H.
EMBO Rep.10:1259-1264(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH UHRF1
22.

"The Dnmt3a PWWP domain reads histone 3 lysine 36 trimethylation and guides DNA methylation."
Dhayalan A. , Rajavelu A. , Rathert P. , Tamas R. , Jurkowska R.Z. , Ragozin S. , Jeltsch A.
J. Biol. Chem.285:26114-26120(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION

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