Doc2b ELISA Kit for Mouse(Mus musculus) | EIAab.comDoc2b ELISA Kit for Mouse(Mus musculus)

Search Protein-oriented Product center.

For antibodies, ELISA/CLIA, proteins... Product center

Tips

Index > Protein center > Doc2b(Gene name) > Mouse

Doc2b (Gene name),

Double C2-like domain-containing protein beta (Protein name ), DOC2B_MOUSE from NCBI database.

top

General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Feedback

Gene name:

Doc2b;

Protein name:

Double C2-like domain-containing protein beta(Doc2-beta);

Alternative:

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Interacts with cytoplasmic dynein light chain DYNLT1. May interact with UNC13A; the interaction mediates targeting to the plasma membrane (By similarity). Probably interacts with the SNARE (soluble N-ethylmaleimide-sensitive factor attached protein receptor) complex composed of SNAP25, STX1A and VAMP2; the interaction is calcium-dependent and competitive with SYT1. Interacts with STX4; the interaction is calcium-dependent, increased by insulin and glucose, and mediates vesicle fusion with plasma membrane in pancretic cells and adipocytes. Interacts with STXBP3; the interaction is direct, occurs at the cell membrane and regulates glucose-stimulated insulin secretion.

Function:

Calcium sensor which positively regulates SNARE-dependent fusion of vesicles with membranes. Binds phospholipids in a calcium-dependent manner and may act at the priming stage of fusion by modifying membrane curvature to stimulate fusion. Involved in calcium-triggered exocytosis in chromaffin cells and calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Involved both in glucose-stimulated insulin secretion in pancreatic cells and insulin-dependent GLUT4 transport to the plasma membrane in adipocytes.

Subcellular Location:

Cytoplasm Cytoplasmic granule Cell membrane Peripheral membrane protein Translocates to the plasma membrane in a calcium-dependent manner.

Protein Attributes:

Sequence length:

412

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

412:

LTNELPGAVL | SD

3D Structure:

N/A

Predicted Eptitope:

Please Sign in.

Vaild Sequence:

Please Sign in.

Related Databases

KEGG:

mmu:13447

SMR:

P70169

Pfam:

PF00168

String:

P70169

UniGene:

Mm.5137

STRING:

10090.ENSMUSP00000021209

Uniprot:

P70169

FOR

ELISA Kit for Mouse Doc2-beta

CLIA Kit for Mouse Doc2-beta

Polyclonal Antibody for Mouse Doc2-beta

Monoclonal Antibody for Mouse Doc2-beta

Protein for Mouse Doc2-beta

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Calcium-dependent phospholipid binding to the C2A domain of a ubiquitous form of double C2 protein (Doc2 beta)."
Kojima T. , Fukuda M. , Aruga J. , Mikoshiba K.
J. Biochem.120:671-676(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];FUNCTION;TISSUE SPECIFICITY
tissue: Cerebellum.
2.

"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M. , Goodstadt L. , Hillier L.W. , Zody M.C. , Goldstein S. , She X. , Bult C.J. , Agarwala R. , Cherry J.L. , DiCuccio M. , Hlavina W. , Kapustin Y. , Meric P. , Maglott D. , Birtle Z. , Marques A.C. , Graves T. , Zhou S. , more...
PLoS Biol.7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
strain: C57BL/6J.
3.

Mural R.J. , Adams M.D. , Myers E.W. , Smith H.O. , Venter J.C.
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
4.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain: C57BL/6.
tissue: Brain.
5.

"Doc2beta is a novel Munc18c-interacting partner and positive effector of syntaxin 4-mediated exocytosis."
Ke B. , Oh E. , Thurmond D.C.
J. Biol. Chem.282:21786-21797(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;INTERACTION WITH STXBP3;TISSUE SPECIFICITY
6.

"DOC2B acts as a calcium switch and enhances vesicle fusion."
Friedrich R. , Groffen A.J. , Connell E. , van Weering J.R. , Gutman O. , Henis Y.I. , Davletov B. , Ashery U.
J. Neurosci.28:6794-6806(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH STX1A AND SNAP25
7.

"DOC2b is a SNARE regulator of glucose-stimulated delayed insulin secretion."
Miyazaki M. , Emoto M. , Fukuda N. , Hatanaka M. , Taguchi A. , Miyamoto S. , Tanizawa Y.
Biochem. Biophys. Res. Commun.384:461-465(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;INTERACTION WITH STX4
8.

"DOC2B: a novel syntaxin-4 binding protein mediating insulin-regulated GLUT4 vesicle fusion in adipocytes."
Fukuda N. , Emoto M. , Nakamori Y. , Taguchi A. , Miyamoto S. , Uraki S. , Oka Y. , Tanizawa Y.
Diabetes58:377-384(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;INTERACTION WITH STX4;MUTAGENESIS OF ASP-157; ASP-163; ASP-297 AND ASP-303
9.

"Doc2b is a high-affinity Ca2+ sensor for spontaneous neurotransmitter release."
Groffen A.J. , Martens S. , Diez Arazola R. , Cornelisse L.N. , Lozovaya N. , de Jong A.P. , Goriounova N.A. , Habets R.L. , Takai Y. , Borst J.G. , Brose N. , McMahon H.T. , Verhage M.
Science327:1614-1618(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISRUPTION PHENOTYPE;FUNCTION

Product Feedback Wall

* Email:
*Comments: