Pola1 ELISA Kit for Mouse(Mus musculus) | EIAab.comPola1 ELISA Kit for Mouse(Mus musculus)

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Pola1 (Gene name),

DNA polymerase alpha catalytic subunit (Protein name ), DPOLA_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Pola1(Pola);

Protein name:

DNA polymerase alpha catalytic subunit;

Alternative:

DNA polymerase alpha catalytic subunit p180;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

The DNA polymerase alpha complex is composed of four subunits: the catalytic subunit POLA1, the regulatory subunit POLA2, and the small and the large primase subunits PRIM1 and PRIM2 respectively. Interacts with PARP1; this interaction functions as part of the control of replication fork progression. Interacts with MCM10 and WDHD1; these interactions recruit the polymerase alpha complex to the pre-replicative complex bound to DNA. Interacts with RPA1; this interaction stabilizes the replicative complex and reduces the misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp.

Function:

Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

Subcellular Location:

Nucleus

Protein Attributes:

Sequence length:

1465

Sequence:

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1450:

1465:

EVNLSKLFAN | YAGKS

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Mm.1923

KEGG:

mmu:18968

String:

P33609

Pfam:

PF12254

SMR:

P33609

Uniprot:

P33609

FOR

ELISA Kit for Mouse DNA polymerase alpha catalytic subunit

CLIA Kit for Mouse DNA polymerase alpha catalytic subunit

Polyclonal Antibody for Mouse DNA polymerase alpha catalytic subunit

Monoclonal Antibody for Mouse DNA polymerase alpha catalytic subunit

Protein for Mouse DNA polymerase alpha catalytic subunit

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Molecular cloning of the cDNAs for the four subunits of mouse DNA polymerase alpha-primase complex and their gene expression during cell proliferation and the cell cycle."
Miyazawa H. , Izumi M. , Tada S. , Takada R. , Masutani M. , Ui M. , Hanaoka F.
J. Biol. Chem.268:8111-8122(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 454-472 AND 1438-1455
2.

"DNA replication in vitro by recombinant DNA-polymerase-alpha-primase."
Stadlbauer F. , Brueckner A. , Rehfuess C. , Eckerskorn C. , Lottspeich F. , Foerster V. , Tseng B.Y. , Nasheuer H.-P.
Eur. J. Biochem.222:781-793(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
3.

"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J. , Chen Y. , Tishkoff D.X. , Peng C. , Tan M. , Dai L. , Xie Z. , Zhang Y. , Zwaans B.M. , Skinner M.E. , Lombard D.B. , Zhao Y.
Mol. Cell50:919-930(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-230;SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-974;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Embryonic fibroblast.
4.

"Identification of a point mutation in the cDNA of the catalytic subunit of DNA polymerase alpha from a temperature-sensitive mouse FM3A cell line."
Izumi M. , Miyazawa H. , Harakawa S. , Yatagai F. , Hanaoka F.
J. Biol. Chem.269:7639-7644(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT PHE-1180

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