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Index > Protein center > EIF2AK1(Gene name) > Human
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  • EIF2AK1 (Gene name),
  • Eukaryotic translation initiation factor 2-alpha kinase 1 (Protein name ),  E2AK1_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    EIF2AK1(PRO1362;HRI;KIAA1369);
    Protein name:
    Eukaryotic translation initiation factor 2-alpha kinase 1;
    Alternative:
    Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase;Heme-controlled repressor(HCR);Hemin-sensitive initiation factor 2-alpha kinase;Heme-regulated inhibitor;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Synthesized in an inactive form that binds to the N-terminal domain of CDC37. Has to be associated with a multiprotein complex containing Hsp90, CDC37 and PPP5C for maturation and activation by autophosphorylation. The phosphatase PPP5C modulates this activation. Forms oligomers. Has been reported as a non-covalently bound homodimer, as well as a hexamer in the absence of hemin. Converted to an inactive disulfide linked homodimer in the presence of hemin.
    Function:
    Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions.
    Subcellular Location:
    Cytoplasm
    Protein Attributes:
    Sequence length:
    630
    Sequence:
    50:
    MQGGNSGVRK | REEEGDGAGA | VAAPPAIDFP | AEGPDPEYDE | SDVPAEIQVL | 
    100:
    KEPLQQPTFP | FAVANQLLLV | SLLEHLSHVH | EPNPLRSRQV | FKLLCQTFIK | 
    150:
    MGLLSSFTCS | DEFSSLRLHH | NRAITHLMRS | AKERVRQDPC | EDISRIQKIR | 
    200:
    SREVALEAQT | SRYLNEFEEL | AILGKGGYGR | VYKVRNKLDG | QYYAIKKILI | 
    250:
    KGATKTVCMK | VLREVKVLAG | LQHPNIVGYH | TAWIEHVHVI | QPRADRAAIE | 
    300:
    LPSLEVLSDQ | EEDREQCGVK | NDESSSSSII | FAEPTPEKEK | RFGESDTENQ | 
    350:
    NNKSVKYTTN | LVIRESGELE | STLELQENGL | AGLSASSIVE | QQLPLRRNSH | 
    400:
    LEESFTSTEE | SSEENVNFLG | QTEAQYHLML | HIQMQLCELS | LWDWIVERNK | 
    450:
    RGREYVDESA | CPYVMANVAT | KIFQELVEGV | FYIHNMGIVH | RDLKPRNIFL | 
    500:
    HGPDQQVKIG | DFGLACTDIL | QKNTDWTNRN | GKRTPTHTSR | VGTCLYASPE | 
    550:
    QLEGSEYDAK | SDMYSLGVVL | LELFQPFGTE | MERAEVLTGL | RTGQLPESLR | 
    600:
    KRCPVQAKYI | QHLTRRNSSQ | RPSAIQLLQS | ELFQNSGNVN | LTLQMKIIEQ | 
    630:
    EKEIAELKKQ | LNLLSQDKGV | RDDGKDGGVG | 
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    UniGene:
    SMR:
    KEGG:
    Pfam:
    String:
    UniGene:
    Uniprot:
     
    FOR
    ELISA Kit for Human Eukaryotic translation initiation factor 2-alpha kinase 1
    ELISA Kit for Human Eukaryotic translation initiation factor 2-alpha kinase 1
    ELISA Kit for Human Eukaryotic translation initiation factor 2-alpha kinase 1
    ELISA Kit for Human Eukaryotic translation initiation factor 2-alpha kinase 1
    CLIA Kit for Human Eukaryotic translation initiation factor 2-alpha kinase 1
    CLIA Kit for Human Eukaryotic translation initiation factor 2-alpha kinase 1
    CLIA Kit for Human Eukaryotic translation initiation factor 2-alpha kinase 1
    CLIA Kit for Human Eukaryotic translation initiation factor 2-alpha kinase 1
    Polyclonal Antibody for Human Eukaryotic translation initiation factor 2-alpha kinase 1
    Polyclonal Antibody for Human Eukaryotic translation initiation factor 2-alpha kinase 1
    Polyclonal Antibody for Human Eukaryotic translation initiation factor 2-alpha kinase 1
    Polyclonal Antibody for Human Eukaryotic translation initiation factor 2-alpha kinase 1
    Monoclonal Antibody for Human Eukaryotic translation initiation factor 2-alpha kinase 1
    Monoclonal Antibody for Human Eukaryotic translation initiation factor 2-alpha kinase 1
    Monoclonal Antibody for Human Eukaryotic translation initiation factor 2-alpha kinase 1
    Monoclonal Antibody for Human Eukaryotic translation initiation factor 2-alpha kinase 1
    Protein for Human Eukaryotic translation initiation factor 2-alpha kinase 1
    Protein for Human Eukaryotic translation initiation factor 2-alpha kinase 1
    Protein for Human Eukaryotic translation initiation factor 2-alpha kinase 1
    Protein for Human Eukaryotic translation initiation factor 2-alpha kinase 1

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    Linearity
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    References
    1. 1.
      "Cloning of hHRI, human heme-regulated eukaryotic initiation factor 2alpha kinase: down-regulated in epithelial ovarian cancers."
      Hwang S.-Y. , Kim M.-K. , Kim J.-C.
      Mol. Cells10:584-591(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
      tissue: Hair follicle dermal papilla.
    2. 2.
      "Cloning and sequencing of a novel human cDNA homologous to rat hemin-sensitive initiation factor 2a kinase mRNA."
      Tu Q. , Yu L. , Hu P.R. , Fu Q. , Cui Y.Y. , Zhao S.Y.
      Submitted (1998-10) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    3. 3.
      "Cloning of the human heme-regulated eukaryotic initiation factor 2-alpha kinase from TNF-alpha stimulated dermal microvascular endothelial cells."
      Cannon G. , Naik S.M. , Boss J.M. , Caughman S.W.
      Submitted (1999-08) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
      tissue: Skin.
    4. 4.
      "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
      Nagase T. , Kikuno R. , Ishikawa K. , Hirosawa M. , Ohara O.
      DNA Res.7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      tissue: Brain.
    5. 5.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      tissue: Hypothalamus.
    6. 6.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
      tissue: Amygdala.
    7. 7.
      "Molecular cloning and sequencing of the human heme-regulated eukaryotic initiation factor 2 alpha (eIF-2 alpha) kinase from bone marrow culture."
      Omasa T. , Chen Y.-G. , Mantalaris A. , Tsai Y.C. , Wu J.H.
      DNA Seq.13:133-137(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)
      tissue: Bone marrow.
    8. 8.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1);VARIANT ARG-558
      tissue: Placenta.
      tissue: Tongue.
    9. 9.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
      tissue: Amygdala.
    10. 11.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    11. 12.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      tissue: Ovary.
    12. 13.
      "Functional prediction of the coding sequences of 121 new genes deduced by analysis of cDNA clones from human fetal liver."
      Zhang C. , Yu Y. , Zhang S. , Wei H. , Zhou G. , Ouyang S. , Luo L. , Bi J. , Liu M. , He F.
      Submitted (1998-12) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 450-630;VARIANT ARG-558
      tissue: Fetal liver.
    13. 14.
      "Hsp90 regulates p50(cdc37) function during the biogenesis of the active conformation of the heme-regulated eIF2 alpha kinase."
      Shao J. , Grammatikakis N. , Scroggins B.T. , Uma S. , Huang W. , Chen J.-J. , Hartson S.D. , Matts R.L.
      J. Biol. Chem.276:206-214(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CDC37 AND THE HSP90 COMPLEX
    14. 15.
      "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H. , Olsen J.V. , Bairlein M. , Gnad F. , Oppermann F.S. , Korner R. , Greff Z. , Keri G. , Stemmann O. , Mann M.
      Mol. Cell31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    15. 16.
      "Hepatic heme-regulated inhibitor (HRI) eukaryotic initiation factor 2alpha kinase: a protagonist of heme-mediated translational control of CYP2B enzymes and a modulator of basal endoplasmic reticulum stress tone."
      Acharya P. , Chen J.J. , Correia M.A.
      Mol. Pharmacol.77:575-592(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY
    16. 17.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    17. 18.
      "Patterns of somatic mutation in human cancer genomes."
      Greenman C. , Stephens P. , Smith R. , Dalgliesh G.L. , Hunter C. , Bignell G. , Davies H. , Teague J. , Butler A. , Stevens C. , Edkins S. , O'Meara S. , Vastrik I. , Schmidt E.E. , Avis T. , Barthorpe S. , Bhamra G. , Buck G. , more...
      Nature446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS [LARGE SCALE ANALYSIS] THR-117; THR-132; LYS-134; SER-139; HIS-145; SER-202; LEU-292; HIS-319 AND ARG-558
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