E2F1 ELISA Kit for Human(Homo sapiens) | EIAab.comE2F1 ELISA Kit for Human(Homo sapiens)

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E2F1 (Gene name),

Transcription factor E2F1 (Protein name ), E2F1_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

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Gene name:

E2F1(RBBP3);

Protein name:

Transcription factor E2F1(E2F-1);

Alternative:

PRB-binding protein E2F-1(RBAP-1);PBR3;Retinoblastoma-binding protein 3(RBBP-3);Retinoblastoma-associated protein 1(RBAP-1);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Viral oncoproteins, notably E1A, T-antigen and HPV E7, are capable of sequestering RB protein, thus releasing the active complex. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Binds TOPBP1 and EAPP. Interacts with ARID3A. Interacts with TRIM28; the interaction inhibits E2F1 acetylation through recruiting HDAC1 and represses its transcriptional activity. Interaction with KAT2B; the interaction acetylates E2F1 enhancing its DNA-binding and transcriptional activity.

Function:

Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.

Subcellular Location:

Nucleus

Protein Attributes:

Sequence length:

437

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

437:

SLSPPHEALD | YHFGLEEGEG | IRDLFDCDFG | DLTPLDF

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Hs.654393

MIM:

189971

Pfam:

PF02319

KEGG:

hsa:1869

SMR:

Q01094

STRING:

9606.ENSP00000345571

String:

Q01094

Uniprot:

Q01094

FOR

ELISA Kit for Human E2F-1

CLIA Kit for Human E2F-1

Polyclonal Antibody for Human E2F-1

Monoclonal Antibody for Human E2F-1

Protein for Human E2F-1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"A cDNA encoding a pRB-binding protein with properties of the transcription factor E2F."
Helin K. , Lees J.A. , Vidal M. , Dyson N.J. , Harlow E. , Fattaey A.
Cell70:337-350(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
2.

"Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties."
Kaelin W.G. Jr. , Krek W. , Sellers W.R. , Decaprio J.A. , Ajchenbaum F. , Fuchs C.S. , Chittenden T. , Li Y. , Farnham P.J. , Blanar M.A. , Livingston D.M. , Flemington E.K.
Cell70:351-364(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
3.

"Molecular cloning of cellular genes encoding retinoblastoma-associated proteins: identification of a gene with properties of the transcription factor E2F."
Shan B. , Zhu X. , Chen P.L. , Durfee T. , Yang Y. , Sharp D. , Lee W.H.
Mol. Cell. Biol.12:5620-5631(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
4.

"Structure and partial genomic sequence of the human E2F1 gene."
Neuman E. , Sellers W.R.S. , McNeil J.A. , Lawrence J.B. , Kaelin W.G. Jr.
Gene173:163-169(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
5.

NIEHS SNPs program
Submitted (2002-05) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS HIS-252; MET-276; ASN-311 AND SER-393
6.

"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P. , Matthews L.H. , Ashurst J.L. , Burton J. , Gilbert J.G.R. , Jones M. , Stavrides G. , Almeida J.P. , Babbage A.K. , Bagguley C.L. , Bailey J. , Barlow K.F. , Bates K.N. , Beard L.M. , Beare D.M. , Beasley O.P. , Bird C.P. , Blakey S.E. , more...
Nature414:865-871(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
7.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Pancreas.
tissue: Skin.
8.

"Autoregulatory control of E2F1 expression in response to positive and negative regulators of cell cycle progression."
Johnson D.G. , Ohtani K. , Nevins J.R.
Genes Dev.8:1514-1525(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-111
9.

"Inhibition of E2F-1 transactivation by direct binding of the retinoblastoma protein."
Helin K. , Harlow E. , Fattaey A.
Mol. Cell. Biol.13:6501-6508(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TRANSACTIVATION INHIBITION;MUTAGENESIS OF TYR-411
10.

"Negative regulation of the growth-promoting transcription factor E2F-1 by a stably bound cyclin A-dependent protein kinase."
Krek W. , Ewen M.E. , Shirodkar S. , Arany Z. , Kaelin W.G. Jr. , Livingston D.M.
Cell78:161-172(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DOMAIN CYCLIN A/CDK2 BINDING
11.

"Differential regulation of E2F transactivation by cyclin/cdk2 complexes."
Dynlacht B.D. , Flores O. , Lees J.A. , Harlow E.
Genes Dev.8:1772-1786(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DIFFERENTIAL REGULATION BY CYCLIN/CDK2 KINASES
12.

"Cyclin A/CDK2 binds directly to E2F-1 and inhibits the DNA-binding activity of E2F-1/DP-1 by phosphorylation."
Xu M. , Sheppard K.-A. , Peng C.-Y. , Yee A.S. , Piwnica-Worms H.
Mol. Cell. Biol.14:8420-8431(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INHIBITION OF DNA-BINDING
13.

"P53 and E2F-1 cooperate to mediate apoptosis."
Wu X. , Levine A.J.
Proc. Natl. Acad. Sci. U.S.A.91:3602-3606(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN APOPTOSIS
14.

"Interaction of the 72-kilodalton human cytomegalovirus IE1 gene product with E2F1 coincides with E2F-dependent activation of dihydrofolate reductase transcription."
Margolis M.J. , Pajovic S. , Wong E.L. , Wade M. , Jupp R. , Nelson J.A. , Azizkhan J.C.
J. Virol.69:7759-7767(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HHV-5 PROTEIN UL123
15.

"Phosphorylation of E2F-1 by cyclin A-cdk2."
Kitagawa M. , Higashi H. , Suzuki-Takahashi I. , Segawa K. , Hanks S.K. , Taya Y. , Nishimura S. , Okuyama A.
Oncogene10:229-236(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION
16.

"Specific regulation of E2F family members by cyclin-dependent kinases."
Dynlacht B.D. , Moberg K. , Lees J.A. , Harlow E. , Zhu L.
Mol. Cell. Biol.17:3867-3875(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REGULATION BY CYCLIN-DEPENDENT KINASES
17.

"A novel E2F binding protein with Myc-type HLH motif stimulates E2F-dependent transcription by forming a heterodimer."
Suzuki M. , Okuyama S. , Okamoto S. , Shirasuna K. , Nakajima T. , Hachiya T. , Nojima H. , Sekiya S. , Oda K.
Oncogene17:853-865(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ARID3A
18.

"Regulation of E2F1 activity by acetylation."
Martinez-Balbas M.A. , Bauer U.M. , Nielsen S.J. , Brehm A. , Kouzarides T.
EMBO J.19:662-671(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION AT LYS-117; LYS-120 AND LYS-125;DNA-BINDING;INTERACTION WITH KAT2B;FUNCTION;MUTAGENESIS OF LYS-117; LYS-120 AND LYS-125
19.

"E2F transcriptional activation requires TRRAP and GCN5 cofactors."
Lang S.E. , McMahon S.B. , Cole M.D. , Hearing P.
J. Biol. Chem.276:32627-32634(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TRRAP
20.

"Regulation of E2F1 by BRCT domain-containing protein TopBP1."
Liu K. , Lin F.-T. , Ruppert J.M. , Lin W.-C.
Mol. Cell. Biol.23:3287-3304(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TOPBP1
21.

"Chk2 activates E2F-1 in response to DNA damage."
Stevens C. , Smith L. , La Thangue N.B.
Nat. Cell Biol.5:401-409(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN TRANSCRIPTION REGULATION;FUNCTION IN APOPTOSIS;PHOSPHORYLATION AT SER-364 BY CHEK2;MUTAGENESIS OF SER-364
22.

"Structure of the Rb C-terminal domain bound to E2F1-DP1: a mechanism for phosphorylation-induced E2F release."
Rubin S.M. , Gall A.-L. , Zheng N. , Pavletich N.P.
Cell123:1093-1106(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH WITH RB1 AND TFDP1
23.

"EAPP, a novel E2F binding protein that modulates E2F-dependent transcription."
Novy M. , Pohn R. , Andorfer P. , Novy-Weiland T. , Galos B. , Schwarzmayr L. , Rotheneder H.
Mol. Biol. Cell16:2181-2190(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH EAPP
24.

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V. , Blagoev B. , Gnad F. , Macek B. , Kumar C. , Mortensen P. , Mann M.
Cell127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-375;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
25.

"Regulation of E2F1 function by the nuclear corepressor KAP1."
Wang C. , Rauscher F.J. III , Cress W.D. , Chen J.
J. Biol. Chem.282:29902-29909(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HDAC1 AND TRIM28;FUNCTION;ACETYLATION;DEACETYLATION
26.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-375;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
27.

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-375;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Leukemic T-cell.
28.

"Cellular inhibitor of apoptosis protein-1 (cIAP1) can regulate E2F1 transcription factor-mediated control of cyclin transcription."
Cartier J. , Berthelet J. , Marivin A. , Gemble S. , Edmond V. , Plenchette S. , Lagrange B. , Hammann A. , Dupoux A. , Delva L. , Eymin B. , Solary E. , Dubrez L.
J. Biol. Chem.286:26406-26417(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH BIRC2/C-IAP1;ENZYME REGULATION
29.

"Specificity determinants of recruitment peptides bound to phospho-CDK2/cyclin A."
Lowe E.D. , Tews I. , Cheng K.Y. , Brown N.R. , Gul S. , Noble M.E.M. , Gamblin S.J. , Johnson L.N.
Biochemistry41:15625-15634(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 87-95
30.

"Crystal structure of the retinoblastoma tumor suppressor protein bound to E2F and the molecular basis of its regulation."
Xiao B. , Spencer J. , Clements A. , Ali-Khan N. , Mittnacht S. , Broceno C. , Burghammer M. , Perrakis A. , Marmorstein R. , Gamblin S.J.
Proc. Natl. Acad. Sci. U.S.A.100:2363-2368(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 409-426 IN COMPLEX WITH RB1

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