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Index > Protein center > EDF1(Gene name) > Human
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  • EDF1 (Gene name),
  • Endothelial differentiation-related factor 1 (Protein name ),  EDF1_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    EDF1;
    Protein name:
    Endothelial differentiation-related factor 1(EDF-1);
    Alternative:
    Multiprotein-bridging factor 1(MBF1);
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Interacts with TBP and the transcription factor IID (TFIID) complex, NR5A2, NR1H3 and PPARG. Interaction with TBP is regulated by phosphorylation. Binds NR5A1, ATF1, FOS and JUN via their conserved basic region. Binding to calmodulin is regulated by calcium and phosphorylation of the IQ motif.
    Function:
    Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism.
    Subcellular Location:
    Cytoplasm Nucleus Also nuclear upon binding to NR5A1 and treatment of cells with TPA or forskolin.
    Protein Attributes:
    Sequence length:
    148
    Sequence:
    50:
    MAESDWDTVT | VLRKKGPTAA | QAKSKQAILA | AQRRGEDVET | SKKWAAGQNK | 
    100:
    QHSITKNTAK | LDRETEELHH | DRVTLEVGKV | IQQGRQSKGL | TQKDLATKIN | 
    148:
    EKPQVIADYE | SGRAIPNNQV | LGKIERAIGL | KLRGKDIGKP | IEKGPRAK
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    UniGene:
    Pfam:
    SMR:
    MIM:
    KEGG:
    String:
    Uniprot:
     
    FOR
    ELISA Kit for Human EDF-1
    ELISA Kit for Human EDF-1
    ELISA Kit for Human EDF-1
    ELISA Kit for Human EDF-1
    ELISA Kit for Human EDF-1
    CLIA Kit for Human EDF-1
    CLIA Kit for Human EDF-1
    CLIA Kit for Human EDF-1
    CLIA Kit for Human EDF-1
    CLIA Kit for Human EDF-1
    Polyclonal Antibody for Human EDF-1
    Polyclonal Antibody for Human EDF-1
    Polyclonal Antibody for Human EDF-1
    Polyclonal Antibody for Human EDF-1
    Polyclonal Antibody for Human EDF-1
    Monoclonal Antibody for Human EDF-1
    Monoclonal Antibody for Human EDF-1
    Monoclonal Antibody for Human EDF-1
    Monoclonal Antibody for Human EDF-1
    Monoclonal Antibody for Human EDF-1
    Protein for Human EDF-1
    Protein for Human EDF-1
    Protein for Human EDF-1
    Protein for Human EDF-1
    Protein for Human EDF-1

    R&D Technical Data
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    Precision
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    Recovery
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    Linearity
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    References
    1. 1.
      "EDF-1, a novel gene product down-regulated in human endothelial cell differentiation."
      Dragoni I. , Mariotti M. , Consalez G.G. , Soria M.R. , Maier J.A.M.
      J. Biol. Chem.273:31119-31124(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);TISSUE SPECIFICITY;DEVELOPMENTAL STAGE;INDUCTION;FUNCTION
    2. 2.
      "The role of human MBF1 as a transcriptional coactivator."
      Kabe Y. , Goto M. , Shima D. , Imai T. , Wada T. , Morohashi K. , Shirakawa M. , Hirose S. , Handa H.
      J. Biol. Chem.274:34196-34202(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2);TISSUE SPECIFICITY;INTERACTION WITH TBP; NR5A1; FOS; JUN AND ATF1;SUBCELLULAR LOCATION;FUNCTION
    3. 3.
      "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
      Submitted (2003-07) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
    4. 4.
      "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Halleck A. , Ebert L. , Mkoundinya M. , Schick M. , Eisenstein S. , Neubert P. , Kstrang K. , Schatten R. , Shen B. , Henze S. , Mar W. , Korn B. , Zuo D. , Hu Y. , LaBaer J.
      Submitted (2004-06) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
    5. 5.
      "DNA sequence and analysis of human chromosome 9."
      Humphray S.J. , Oliver K. , Hunt A.R. , Plumb R.W. , Loveland J.E. , Howe K.L. , Andrews T.D. , Searle S. , Hunt S.E. , Scott C.E. , Jones M.C. , Ainscough R. , Almeida J.P. , Ambrose K.D. , Ashwell R.I.S. , Babbage A.K. , Babbage S. , Bagguley C.L. , more...
      Nature429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    6. 6.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      tissue: Pancreas.
    7. 7.
      Bienvenut W.V. , Waridel P. , Quadroni M.
      Submitted (2009-03) to the UniProtKB
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-13 AND 99-113;CLEAVAGE OF INITIATOR METHIONINE;ACETYLATION AT ALA-2;IDENTIFICATION BY MASS SPECTROMETRY
      tissue: Cervix carcinoma.
    8. 8.
      "Interaction between endothelial differentiation-related factor-1 and calmodulin in vitro and in vivo."
      Mariotti M. , De Benedictis L. , Avon E. , Maier J.A.M.
      J. Biol. Chem.275:24047-24051(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CALM AND TBP;MUTAGENESIS OF THR-40; THR-58; THR-91 AND SER-111;PHOSPHORYLATION;SUBCELLULAR LOCATION
    9. 9.
      "Multiprotein bridging factor-1 (MBF-1) is a cofactor for nuclear receptors that regulate lipid metabolism."
      Brendel C. , Gelman L. , Auwerx J.
      Mol. Endocrinol.16:1367-1377(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH NR5A2; NR1H3; PPARG AND TFIID COMPLEX;TISSUE SPECIFICITY;FUNCTION
    10. 10.
      "The dual role of endothelial differentiation-related factor-1 in the cytosol and nucleus: modulation by protein kinase A."
      Ballabio E. , Mariotti M. , De Benedictis L. , Maier J.A.M.
      Cell. Mol. Life Sci.61:1069-1074(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF THR-65; THR-74 AND SER-87;INTERACTION WITH TBP AND CALM;PHOSPHORYLATION;SUBCELLULAR LOCATION;FUNCTION
    11. 11.
      "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
      Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    12. 12.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    13. 13.
      "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V. , Sumpton D. , Martinez A. , Lilla S. , Espagne C. , Meinnel T. , Giglione C.
      Mol. Cell. Proteomics11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    14. 14.
      "Solution structures of the HTH domain of human EDF-1 protein."
      RIKEN structural genomics initiative (RSGI)
      Submitted (2005-11) to the PDB data bank
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 71-148
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