Early growth response protein 1 (Protein name
), EGR1_BOVIN from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Gene name:
EGR1;
Protein name:
Early growth response protein 1(EGR-1);
Alternative:
Organism:
Bovine (Bos taurus).
General Annotation
Sub Unit:
N/A
Function:
Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.6%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.9%
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Recovery
Recovery was determined by spiking various levels of EGR1 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
101
96-103
Plasma
100
98-102
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EGR1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
96-103%
89-98%
102-109%
100-109%
EDTA plasma(n=5)
93-99%
103-108%
94-98%
96-102%
heparin plasma(n=5)
98-104%
100-106%
95-104%
93-100%
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References
1.
"Characterization of bovine early growth response 1." Sayasith K.
,
Sirois J.
Submitted (2005-02) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [MRNA]