EHMT2 ELISA Kit for Human(Homo sapiens) | EIAab.comEHMT2 ELISA Kit for Human(Homo sapiens) | EIAab.com

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EHMT2 (Gene name),

Histone-lysine N-methyltransferase EHMT2 (Protein name ), EHMT2_HUMAN from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Gene name:

EHMT2(BAT8;C6orf30;G9A;KMT1C;NG36);

Protein name:

Histone-lysine N-methyltransferase EHMT2;

Alternative:

HLA-B-associated transcript 8;Euchromatic histone-lysine N-methyltransferase 2;Lysine N-methyltransferase 1C;Histone H3-K9 methyltransferase 3(H3-K9-HMTase 3);Protein G9a;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Heterodimer; heterodimerizes with EHMT1/GLP. Interacts with GFI1B and WIZ. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EHMT1, RING1, RNF2, MBLR, L3MBTL2 and YAF2.

Function:

Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.

Subcellular Location:

Nucleus Chromosome Associates with euchromatic regions. Does not associate with heterochromatin.

Protein Attributes:

Sequence length:

1210

Sequence:

50:

MAAAAGAAAA | AAAEGEAPAE | MGALLLEKET | RGATERVHGS | LGDTPRSEET |

100:

LPKATPDSLE | PAGPSSPASV | TVTVGDEGAD | TPVGATPLIG | DESENLEGDG |

150:

DLRGGRILLG | HATKSFPSSP | SKGGSCPSRA | KMSMTGAGKS | PPSVQSLAMR |

200:

LLSMPGAQGA | AAAGSEPPPA | TTSPEGQPKV | HRARKTMSKP | GNGQPPVPEK |

250:

RPPEIQHFRM | SDDVHSLGKV | TSDLAKRRKL | NSGGGLSEEL | GSARRSGEVT |

300:

LTKGDPGSLE | EWETVVGDDF | SLYYDSYSVD | ERVDSDSKSE | VEALTEQLSE |

350:

EEEEEEEEEE | EEEEEEEEEE | EEEDEESGNQ | SDRSGSSGRR | KAKKKWRKDS |

400:

PWVKPSRKRR | KREPPRAKEP | RGVNGVGSSG | PSEYMEVPLG | SLELPSEGTL |

450:

SPNHAGVSND | TSSLETERGF | EELPLCSCRM | EAPKIDRISE | RAGHKCMATE |

500:

SVDGELSGCN | AAILKRETMR | PSSRVALMVL | CETHRARMVK | HHCCPGCGYF |

550:

CTAGTFLECH | PDFRVAHRFH | KACVSQLNGM | VFCPHCGEDA | SEAQEVTIPR |

600:

GDGVTPPAGT | AAPAPPPLSQ | DVPGRADTSQ | PSARMRGHGE | PRRPPCDPLA |

650:

DTIDSSGPSL | TLPNGGCLSA | VGLPLGPGRE | ALEKALVIQE | SERRKKLRFH |

700:

PRQLYLSVKQ | GELQKVILML | LDNLDPNFQS | DQQSKRTPLH | AAAQKGSVEI |

750:

CHVLLQAGAN | INAVDKQQRT | PLMEAVVNNH | LEVARYMVQR | GGCVYSKEED |

800:

GSTCLHHAAK | IGNLEMVSLL | LSTGQVDVNA | QDSGGWTPII | WAAEHKHIEV |

850:

IRMLLTRGAD | VTLTDNEENI | CLHWASFTGS | AAIAEVLLNA | RCDLHAVNYH |

900:

GDTPLHIAAR | ESYHDCVLLF | LSRGANPELR | NKEGDTAWDL | TPERSDVWFA |

950:

LQLNRKLRLG | VGNRAIRTEK | IICRDVARGY | ENVPIPCVNG | VDGEPCPEDY |

1000:

KYISENCETS | TMNIDRNITH | LQHCTCVDDC | SSSNCLCGQL | SIRCWYDKDG |

1050:

RLLQEFNKIE | PPLIFECNQA | CSCWRNCKNR | VVQSGIKVRL | QLYRTAKMGW |

1100:

GVRALQTIPQ | GTFICEYVGE | LISDAEADVR | EDDSYLFDLD | NKDGEVYCID |

1150:

ARYYGNISRF | INHLCDPNII | PVRVFMLHQD | LRFPRIAFFS | SRDIRTGEEL |

1200:

GFDYGDRFWD | IKSKYFTCQC | GSEKCKHSAE | AIALEQSRLA | RLDPHPELLP |

1210:

ELGSLPPVNT |

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

SMR:

String:

KEGG:

Pfam:

STRING:

9606.ENSP00000397323

MIM:

Uniprot:

FOR

ELISA Kit for Human Histone-lysine N-methyltransferase EHMT2

ELISA Kit for Human Histone-lysine N-methyltransferase EHMT2

CLIA Kit for Human Histone-lysine N-methyltransferase EHMT2

CLIA Kit for Human Histone-lysine N-methyltransferase EHMT2

Polyclonal Antibody for Human Histone-lysine N-methyltransferase EHMT2

Polyclonal Antibody for Human Histone-lysine N-methyltransferase EHMT2

Monoclonal Antibody for Human Histone-lysine N-methyltransferase EHMT2

Monoclonal Antibody for Human Histone-lysine N-methyltransferase EHMT2

Protein for Human Histone-lysine N-methyltransferase EHMT2

Protein for Human Histone-lysine N-methyltransferase EHMT2

R&D Technical Data

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Precision

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Recovery

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Linearity

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References

1.

"Novel NG36/G9a gene products encoded within the human and mouse MHC class III regions."
Brown S.E. , Campbell R.D. , Sanderson C.M.
Mamm. Genome12:916-924(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);ALTERNATIVE SPLICING (ISOFORM 2);TISSUE SPECIFICITY;VARIANT ASN-55
2.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3)
tissue: Salivary gland.
3.

"Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse."
Xie T. , Rowen L. , Aguado B. , Ahearn M.E. , Madan A. , Qin S. , Campbell R.D. , Hood L.
Genome Res.13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
4.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
5.

"Homo sapiens 2,229,817bp genomic DNA of 6p21.3 HLA class I region."
Hirakawa M. , Yamaguchi H. , Imai K. , Shimada J. , Shiina S. , Tamiya G. , Oka A. , Inoko H.
Submitted (1999-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA];VARIANT ASN-55
6.

"The DNA sequence and analysis of human chromosome 6."
Mungall A.J. , Palmer S.A. , Sims S.K. , Edwards C.A. , Ashurst J.L. , Wilming L. , Jones M.C. , Horton R. , Hunt S.E. , Scott C.E. , Gilbert J.G.R. , Clamp M.E. , Bethel G. , Milne S. , Ainscough R. , Almeida J.P. , Ambrose K.D. , Andrews T.D. , more...
Nature425:805-811(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA];VARIANT ASN-55
7.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 6-1210 (ISOFORM 1);VARIANT ASN-55
tissue: Muscle.
tissue: Uterus.
8.

"The G9a gene in the human major histocompatibility complex encodes a novel protein containing ankyrin-like repeats."
Milner C.M. , Campbell R.D.
Biochem. J.290:811-818(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 195-1210;FUNCTION
tissue: Histiocytic lymphoma.
9.

"Set domain-containing protein, G9a, is a novel lysine-preferring mammalian histone methyltransferase with hyperactivity and specific selectivity to lysines 9 and 27 of histone H3."
Tachibana M. , Sugimoto K. , Fukushima T. , Shinkai Y.
J. Biol. Chem.276:25309-25317(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CATALYTIC ACTIVITY;FUNCTION;SUBCELLULAR LOCATION
10.

"A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells."
Ogawa H. , Ishiguro K. , Gaubatz S. , Livingston D.M. , Nakatani Y.
Science296:1132-1136(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION IN COMPLEX WITH E2F6; TFDP1; MAX; MGA; EHMT1; CBX3; RING1; RNF2; MBLR; L3MBTL2 AND YAF2
11.

"Gfi1b alters histone methylation at target gene promoters and sites of gamma-satellite containing heterochromatin."
Vassen L. , Fiolka K. , Moeroey T.
EMBO J.25:2409-2419(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH GFI1B
12.

"Zinc finger protein Wiz links G9a/GLP histone methyltransferases to the co-repressor molecule CtBP."
Ueda J. , Tachibana M. , Ikura T. , Shinkai Y.
J. Biol. Chem.281:20120-20128(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH WIZ AND EHMT1
13.

"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A. , Villen J. , Gerber S.A. , Rush J. , Gygi S.P.
Nat. Biotechnol.24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-246;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
14.

"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S. , Ballif B.A. , Smogorzewska A. , McDonald E.R. III , Hurov K.E. , Luo J. , Bakalarski C.E. , Zhao Z. , Solimini N. , Lerenthal Y. , Shiloh Y. , Gygi S.P. , Elledge S.J.
Science316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-569;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Embryonic kidney.
15.

"CDYL bridges REST and histone methyltransferases for gene repression and suppression of cellular transformation."
Mulligan P. , Westbrook T.F. , Ottinger M. , Pavlova N. , Chang B. , Macia E. , Shi Y.J. , Barretina J. , Liu J. , Howley P.M. , Elledge S.J. , Shi Y.
Mol. Cell32:718-726(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CDYL AND REST;IDENTIFICATION IN A COMPLEX WITH REST; CDYL; SETB1; EHMT1 AND WIZ
16.

"Protein lysine methyltransferase G9a acts on non-histone targets."
Rathert P. , Dhayalan A. , Murakami M. , Zhang X. , Tamas R. , Jurkowska R. , Komatsu Y. , Shinkai Y. , Cheng X. , Jeltsch A.
Nat. Chem. Biol.4:344-346(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;METHYLATION AT LYS-185;IDENTIFICATION BY MASS SPECTROMETRY
17.

"The ankyrin repeats of G9a and GLP histone methyltransferases are mono-and dimethyllysine binding modules."
Collins R.E. , Northrop J.P. , Horton J.R. , Lee D.Y. , Zhang X. , Stallcup M.R. , Cheng X.
Nat. Struct. Mol. Biol.15:245-250(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DOMAIN ANK REPEATS;MUTAGENESIS OF TRP-786; TRP-791; GLU-794; GLU-817; TRP-824 AND ASP-852
18.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-140; SER-173; SER-232; SER-246 AND THR-1210;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
19.

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS];CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS]
20.

"UHRF1 binds G9a and participates in p21 transcriptional regulation in mammalian cells."
Kim J.K. , Esteve P.O. , Jacobsen S.E. , Pradhan S.
Nucleic Acids Res.37:493-505(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH UHRF1
21.

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Leukemic T-cell.
22.

"G9a and Glp methylate lysine 373 in the tumor suppressor p53."
Huang J. , Dorsey J. , Chuikov S. , Perez-Burgos L. , Zhang X. , Jenuwein T. , Reinberg D. , Berger S.L.
J. Biol. Chem.285:9636-9641(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH TP53
23.

Huang J. , Dorsey J. , Chuikov S. , Perez-Burgos L. , Zhang X. , Jenuwein T. , Reinberg D. , Berger S.L.
J. Biol. Chem.285:18122-18122(2010)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM
24.

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-140; SER-232 AND SER-246;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
25.

"Maintenance of silent chromatin through replication requires SWI/SNF-like chromatin remodeler SMARCAD1."
Rowbotham S.P. , Barki L. , Neves-Costa A. , Santos F. , Dean W. , Hawkes N. , Choudhary P. , Will W.R. , Webster J. , Oxley D. , Green C.M. , Varga-Weisz P. , Mermoud J.E.
Mol. Cell42:285-296(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SMARCAD1
26.

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T. , Prokhorova T.A. , Akimov V. , Henningsen J. , Johansen P.T. , Kratchmarova I. , Kassem M. , Mann M. , Olsen J.V. , Blagoev B.
Sci. Signal.4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-140; SER-232; SER-246; SER-413 AND SER-1204;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
27.

"Histone H3 lysine 56 methylation regulates DNA replication through its interaction with PCNA."
Yu Y. , Song C. , Zhang Q. , Dimaggio P.A. , Garcia B.A. , York A. , Carey M.F. , Grunstein M.
Mol. Cell46:7-17(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
28.

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P. , Lasa M. , Polevoda B. , Gazquez C. , Elosegui-Artola A. , Kim D.S. , De Juan-Pardo E. , Demeyer K. , Hole K. , Larrea E. , Timmerman E. , Prieto J. , Arnesen T. , Sherman F. , Gevaert K. , Aldabe R.
Proc. Natl. Acad. Sci. U.S.A.109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
29.

"Structural biology of human H3K9 methyltransferases."
Wu H. , Min J. , Lunin V.V. , Antoshenko T. , Dombrovski L. , Zeng H. , Allali-Hassani A. , Campagna-Slater V. , Vedadi M. , Arrowsmith C.H. , Plotnikov A.N. , Schapira M.
PLoS ONE5:E8570-E8570(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 913-1193 IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE AND ZINC IONS;FUNCTION;CATALYTIC ACTIVITY

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