EIF3K ELISA Kit for Human(Homo sapiens) | EIAab.comEIF3K ELISA Kit for Human(Homo sapiens) | EIAab.com

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EIF3K (Gene name),

Eukaryotic translation initiation factor 3 subunit K (Protein name ), EIF3K_HUMAN from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

EIF3K(ARG134;HSPC029;MSTP001;PTD001;EIF3S12);

Protein name:

Eukaryotic translation initiation factor 3 subunit K(eIF3k);

Alternative:

Muscle-specific gene M9 protein;Eukaryotic translation initiation factor 3 subunit 12;eIF-3 p25;PLAC-24;eIF-3 p28;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with CCND3, but not with CCND1 and CCND2.

Function:

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Subcellular Location:

Nucleus Cytoplasm

Protein Attributes:

Sequence length:

218

Sequence:

50:

MAMFEQMRAN | VGKLLKGIDR | YNPENLATLE | RYVETQAKEN | AYDLEANLAV |

100:

LKLYQFNPAF | FQTTVTAQIL | LKALTNLPHT | DFTLCKCMID | QAHQEERPIR |

150:

QILYLGDLLE | TCHFQAFWQA | LDENMDLLEG | ITGFEDSVRK | FICHVVGITY |

200:

QHIDRWLLAE | MLGDLSDSQL | KVWMSKYGWS | ADESGQIFIC | SQEESIKPKN |

218:

IVEKIDFDSV | SSIMASSQ

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

SMR:

STRING:

9606.ENSP00000248342

MIM:

KEGG:

String:

Pfam:

Uniprot:

FOR

ELISA Kit for Human eIF3k

ELISA Kit for Human eIF3k

ELISA Kit for Human eIF3k

CLIA Kit for Human eIF3k

CLIA Kit for Human eIF3k

CLIA Kit for Human eIF3k

Polyclonal Antibody for Human eIF3k

Polyclonal Antibody for Human eIF3k

Polyclonal Antibody for Human eIF3k

Monoclonal Antibody for Human eIF3k

Monoclonal Antibody for Human eIF3k

Monoclonal Antibody for Human eIF3k

Protein for Human eIF3k

Protein for Human eIF3k

Protein for Human eIF3k

R&D Technical Data

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Precision

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Recovery

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Linearity

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References

1.

"Characterization of eIF3k: a newly discovered subunit of mammalian translation initiation factor eIF3."
Mayeur G.L. , Fraser C.S. , Peiretti F. , Block K.L. , Hershey J.W.B.
Eur. J. Biochem.270:4133-4139(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);PROTEIN SEQUENCE OF 21-31;IDENTIFICATION IN THE EIF-3 COMPLEX;INTERACTION WITH EIF3B; EIF3C; EIF3G AND EIF3J;TISSUE SPECIFICITY;BLOCKAGE OF N-TERMINUS
tissue: Cervix carcinoma.
2.

"Muscle specific gene M9."
Nawa G. , Miyoshi Y. , Nakamura Y.
Submitted (1998-10) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
tissue: Skeletal muscle.
3.

Ye M. , Song H. , Peng Y. , Huang Q. , Dai M. , Mao Y. , Zhu H. , Li G. , Luo M. , Hu R. , Chen J.
Submitted (1998-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
tissue: Pituitary tumor.
4.

Hui R.T. , Liu Y.Q. , Liu B. , Zhao B. , Meng X.M. , Sheng H. , Xu Y.Y. , Wang X.Y. , Ye J. , Song L. , Gao Y. , Wei Y.J. , Zhang C.L. , Zhang J. , Chai M.Q. , Chen J.Z. , Sun Y.H. , Zhou X.L. , more...
Submitted (1998-11) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
tissue: Aorta.
5.

"Gene cloning of human adenocarcinoma cell line."
Yanqiu Z. , Huazhang A. , Fei L. , Baojun C. , Taidong Q. , Kaichun W. , Jie D. , Daiming F.
Submitted (2000-10) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
tissue: Stomach cancer.
6.

"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Zhang Q.-H. , Ye M. , Wu X.-Y. , Ren S.-X. , Zhao M. , Zhao C.-J. , Fu G. , Shen Y. , Fan H.-Y. , Lu G. , Zhong M. , Xu X.-R. , Han Z.-G. , Zhang J.-W. , Tao J. , Huang Q.-H. , Zhou J. , Hu G.-X. , more...
Genome Res.10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
tissue: Umbilical cord blood.
7.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2)
tissue: Cerebellum.
tissue: Spleen.
8.

"The DNA sequence and biology of human chromosome 19."
Grimwood J. , Gordon L.A. , Olsen A.S. , Terry A. , Schmutz J. , Lamerdin J.E. , Hellsten U. , Goodstein D. , Couronne O. , Tran-Gyamfi M. , Aerts A. , Altherr M. , Ashworth L. , Bajorek E. , Black S. , Branscomb E. , Caenepeel S. , Carrano A.V. , more...
Nature428:529-535(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
9.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
10.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
tissue: Placenta.
tissue: Skin.
tissue: Uterus.
11.

Bienvenut W.V. , Zebisch A. , Kolch W.
Submitted (2008-12) to the UniProtKB

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-8; 21-31 AND 39-52;CLEAVAGE OF INITIATOR METHIONINE;ACETYLATION AT ALA-2;IDENTIFICATION BY MASS SPECTROMETRY
tissue: Colon carcinoma.
12.

"Identification of the p28 subunit of eukaryotic initiation factor 3(eIF3k) as a new interaction partner of cyclin D3."
Shen X. , Yang Y. , Liu W. , Sun M. , Jiang J. , Zong H. , Gu J.
FEBS Lett.573:139-146(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CCND3;SUBCELLULAR LOCATION
13.

"Binding of eukaryotic initiation factor 3 to ribosomal 40S subunits and its role in ribosomal dissociation and anti-association."
Kolupaeva V.G. , Unbehaun A. , Lomakin I.B. , Hellen C.U.T. , Pestova T.V.
RNA11:470-486(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION OF THE EIF-3 COMPLEX
14.

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V. , Blagoev B. , Gnad F. , Macek B. , Kumar C. , Mortensen P. , Mann M.
Cell127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
15.

"Translation initiation factor eIF4G-1 binds to eIF3 through the eIF3e subunit."
LeFebvre A.K. , Korneeva N.L. , Trutschl M. , Cvek U. , Duzan R.D. , Bradley C.A. , Hershey J.W.B. , Rhoads R.E.
J. Biol. Chem.281:22917-22932(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION IN THE EIF-3 COMPLEX;IDENTIFICATION BY MASS SPECTROMETRY
16.

"Reconstitution reveals the functional core of mammalian eIF3."
Masutani M. , Sonenberg N. , Yokoyama S. , Imataka H.
EMBO J.26:3373-3383(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION OF THE EIF-3 COMPLEX
17.

"Structural characterization of the human eukaryotic initiation factor 3 protein complex by mass spectrometry."
Damoc E. , Fraser C.S. , Zhou M. , Videler H. , Mayeur G.L. , Hershey J.W.B. , Doudna J.A. , Robinson C.V. , Leary J.A.
Mol. Cell. Proteomics6:1135-1146(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION IN THE EIF-3 COMPLEX;CHARACTERIZATION OF THE EIF-3 COMPLEX;CLEAVAGE OF INITIATOR METHIONINE;ACETYLATION AT ALA-2;MASS SPECTROMETRY
18.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-217;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
19.

"Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3."
Zhou M. , Sandercock A.M. , Fraser C.S. , Ridlova G. , Stephens E. , Schenauer M.R. , Yokoi-Fong T. , Barsky D. , Leary J.A. , Hershey J.W.B. , Doudna J.A. , Robinson C.V.
Proc. Natl. Acad. Sci. U.S.A.105:18139-18144(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION IN THE EIF-3 COMPLEX;CHARACTERIZATION OF THE EIF-3 COMPLEX;MASS SPECTROMETRY
20.

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
21.

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-217;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
22.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
23.

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T. , Prokhorova T.A. , Akimov V. , Henningsen J. , Johansen P.T. , Kratchmarova I. , Kassem M. , Mann M. , Olsen J.V. , Blagoev B.
Sci. Signal.4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-217;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
24.

"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V. , Sumpton D. , Martinez A. , Lilla S. , Espagne C. , Meinnel T. , Giglione C.
Mol. Cell. Proteomics11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
25.

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P. , Lasa M. , Polevoda B. , Gazquez C. , Elosegui-Artola A. , Kim D.S. , De Juan-Pardo E. , Demeyer K. , Hole K. , Larrea E. , Timmerman E. , Prieto J. , Arnesen T. , Sherman F. , Gevaert K. , Aldabe R.
Proc. Natl. Acad. Sci. U.S.A.109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
26.

"Crystal structure of human eIF3k, the first structure of eIF3 subunits."
Wei Z. , Zhang P. , Zhou Z. , Cheng Z. , Wan M. , Gong W.
J. Biol. Chem.279:34983-34990(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS)

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