Skp2 ELISA Kit for Mouse(Mus musculus) | EIAab.comSkp2 ELISA Kit for Mouse(Mus musculus)

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Skp2 (Gene name),

S-phase kinase-associated protein 2 (Protein name ), SKP2_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Skp2;

Protein name:

S-phase kinase-associated protein 2;

Alternative:

F-box protein Skp2;Cyclin-A/CDK2-associated protein p45;F-box/WD-40 protein 1(FWD1);

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Part of a SCF(SKP2) complex consisting of CUL1, RBX1, SKP1 and SKP2. Component of a SCF(SKP2)-like complex containing CUL1, SKP1, TRIM21 and SKP2. Interacts directly with CUL1 and SKP1. Interacts with CKS1. Interacts with the cyclin-A-CDK2 complex. Interacts with ORC1, phosphorylated CDT1, phosphorylated RBL2, ELF4, phosphorylated RAG2, FOXO1A, UBP43, MYC, TOB1, TAL1 and MLL. Interacts with TRIM21.

Function:

Substrate recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. The SCF complex provides substrate specificity and interacts with both, the E2 ubiquitin-conjugating enzyme and the substrate. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1.

Subcellular Location:

N/A

Protein Attributes:

Sequence length:

424

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

424:

MDSKKNLEIW | GIKCRLTLQK | PSCL

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

Q9Z0Z3

UniGene:

Mm.35584

Pfam:

PF00646

SMR:

Q9Z0Z3

KEGG:

mmu:27401

Uniprot:

Q9Z0Z3

FOR

ELISA Kit for Mouse S-phase kinase-associated protein 2

Cat.:

E9870h

Price:

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Manual:

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MSDS:

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Packing:

96T

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ELISA Kit for Mouse S-phase kinase-associated protein 2

Cat.:

E9870m

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Mouse S-phase kinase-associated protein 2

Cat.:

U9870h

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Mouse S-phase kinase-associated protein 2

Cat.:

U9870m

Price:

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MSDS:

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Packing:

96T

Polyclonal Antibody for Mouse S-phase kinase-associated protein 2

Cat.:

P9870Rb-m

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse S-phase kinase-associated protein 2

Cat.:

P9870Rb-h

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Mouse S-phase kinase-associated protein 2

Protein for Mouse S-phase kinase-associated protein 2

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.6%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	100	92-103
Plasma	103	95-107

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

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