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Index > Protein center > Srsf1(Gene name) > Mouse
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  • Srsf1 (Gene name),
  • Serine/arginine-rich splicing factor 1 (Protein name ),  SRSF1_MOUSE from NCBI database.
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  • Gene name:
    Srsf1(Sfrs1);
    Protein name:
    Serine/arginine-rich splicing factor 1;
    Alternative:
    Pre-mRNA-splicing factor SRp30a;ASF/SF2;Splicing factor, arginine/serine-rich 1;
    Organism:
    Mouse (Mus musculus). 
    General Annotation
    Sub Unit:
    Consists of two polypeptides of p32 and p33. In vitro, self-associates and binds SRSF2, SNRNP70 and U2AF1 but not U2AF2. Binds SREK1/SFRS12. Interacts with SAFB/SAFB1. Interacts with SRPK1. Interacts with PSIP1/LEDGF. Identified in the spliceosome C complex. Interacts with RSRC1 (via Arg/Ser-rich domain). Interacts with ZRSR2/U2AF1-RS2. Interacts with CCDC55 (via C-terminus).
    Function:
    Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing (By similarity). Specifically regulates alternative splicing of cardiac isoforms of CAMK2D, LDB3/CYPHER and TNNT2/CTNT during heart remodeling at the juvenile to adult transition. The inappropriate accumulation of a neonatal and neuronal isoform of CAMKD2 in the adult heart results in aberrant calcium handling and defective excitation-contraction coupling in cardiomyocytes.
    Subcellular Location:
    Cytoplasm Nucleus Nucleus speckle Shuttles between the nucleus and the cytoplasm.
    Protein Attributes:
    Sequence length:
    248
    Sequence:
    50:
    MSGGGVIRGP | AGNNDCRIYV | GNLPPDIRTK | DIEDVFYKYG | AIRDIDLKNR | 
    100:
    RGGPPFAFVE | FEDPRDAEDA | VYGRDGYDYD | GYRLRVEFPR | SGRGTGRGGG | 
    150:
    GGGGGGAPRG | RYGPPSRRSE | NRVVVSGLPP | SGSWQDLKDH | MREAGDVCYA | 
    200:
    DVYRDGTGVV | EFVRKEDMTY | AVRKLDNTKF | RSHEGETAYI | RVKVDGPRSP | 
    248:
    SYGRSRSRSR | SRSRSRSRSN | SRSRSYSPRR | SRGSPRYSPR | HSRSRSRT
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    String:
    SMR:
    Pfam:
    UniGene:
    Uniprot:
     
    FOR
    ELISA Kit for Mouse Serine/arginine-rich splicing factor 1
    Cat.:
    E8282h
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Mouse Serine/arginine-rich splicing factor 1
    Cat.:
    E8282c
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    MSDS:
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    96T
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    ELISA Kit for Mouse Serine/arginine-rich splicing factor 1
    Cat.:
    E8282m
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Mouse Serine/arginine-rich splicing factor 1
    Cat.:
    E8282p
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Mouse Serine/arginine-rich splicing factor 1
    Cat.:
    E8282b
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    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse Serine/arginine-rich splicing factor 1
    Cat.:
    U8282m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse Serine/arginine-rich splicing factor 1
    Cat.:
    U8282p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse Serine/arginine-rich splicing factor 1
    MSDS:
    Please sign in first.
    CLIA Kit for Mouse Serine/arginine-rich splicing factor 1
    Cat.:
    U8282h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Mouse Serine/arginine-rich splicing factor 1
    Cat.:
    U8282b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Mouse Serine/arginine-rich splicing factor 1
    Cat.:
    P8282Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse Serine/arginine-rich splicing factor 1
    Polyclonal Antibody for Mouse Serine/arginine-rich splicing factor 1
    Polyclonal Antibody for Mouse Serine/arginine-rich splicing factor 1
    Cat.:
    P8282Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse Serine/arginine-rich splicing factor 1
    Monoclonal Antibody for Mouse Serine/arginine-rich splicing factor 1
    Monoclonal Antibody for Mouse Serine/arginine-rich splicing factor 1
    Monoclonal Antibody for Mouse Serine/arginine-rich splicing factor 1
    Monoclonal Antibody for Mouse Serine/arginine-rich splicing factor 1
    Monoclonal Antibody for Mouse Serine/arginine-rich splicing factor 1
    Protein for Mouse Serine/arginine-rich splicing factor 1
    Protein for Mouse Serine/arginine-rich splicing factor 1
    Protein for Mouse Serine/arginine-rich splicing factor 1
    Protein for Mouse Serine/arginine-rich splicing factor 1
    Protein for Mouse Serine/arginine-rich splicing factor 1

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    References
    1. 1.
      "The transcriptional landscape of the mammalian genome."
      Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
      Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2)
      strain: C57BL/6J.
      tissue: Bone marrow macrophage.
      tissue: Eye.
      tissue: Medulla oblongata.
    2. 2.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
      strain: C57BL/6J.
    3. 3.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      strain: C57BL/6.
      tissue: Embryonic brain.
    4. 4.
      Lubec G. , Yang J.W. , Zigmond M.
      Submitted (2007-07) to the UniProtKB
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 18-28
      tissue: Brain.
    5. 5.
      "Characterization and comparison of four serine- and arginine-rich (SR) protein kinases."
      Nayler O. , Stamm S. , Ullrich A.
      Biochem. J.326:693-700(1997) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION BY CLK1; CLK2; CLK3 AND CLK4
    6. 6.
      "ASF/SF2-regulated CaMKIIdelta alternative splicing temporally reprograms excitation-contraction coupling in cardiac muscle."
      Xu X. , Yang D. , Ding J.-H. , Wang W. , Chu P.-H. , Dalton N.D. , Wang H.-Y. , Bermingham J.R. Jr. , Ye Z. , Liu F. , Rosenfeld M.G. , Manley J.L. , Ross J. Jr. , Chen J. , Xiao R.-P. , Cheng H. , Fu X.-D.
      Cell120:59-72(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    7. 7.
      "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M. , Bailey C.M. , Cunningham D.L. , Heath J.K. , Cooper H.J.
      Mol. Cell. Proteomics8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199 AND SER-201;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Embryonic fibroblast.
    8. 8.
      "Solution structure of RRM domain in splicing factor 2."
      RIKEN structural genomics initiative (RSGI)
      Submitted (2005-11) to the PDB data bank
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 113-207
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