FGF1 ELISA Kit for Bovine(Bos taurus) | EIAab.comFGF1 ELISA Kit for Bovine(Bos taurus)

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Index > Protein center > FGF1(Gene name) > Bovine

FGF1 (Gene name),

Heparin-binding growth factor 1 (Protein name ), FGF1_BOVIN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

FGF1(AFGF;FGF-1;FGFA;HBGF-1);

Protein name:

Heparin-binding growth factor 1(HBGF-1);

Alternative:

Acidic fibroblast growth factor(aFGF);Acidic eye-derived growth factor II(EDGF II);Prostatropin(HBGF-1);Endothelial cell growth factor beta and alpha chains(ECGF);Prostatropin;

Organism:

Bovine (Bos taurus).

General Annotation

Sub Unit:

Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with S100A13. Interacts with FGFBP1 (By similarity). Interacts with SYT1.

Function:

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.

Subcellular Location:

Secreted Cytoplasm Cytoplasm cell cortex Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.

Protein Attributes:

Sequence length:

155

Sequence:

50:

100:

150:

155:

PVSSD

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

bta:281160

SMR:

P03968

String:

P03968

STRING:

9913.ENSBTAP00000040651

Pfam:

PF00167

UniGene:

Bt.5038

Uniprot:

P03968

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ELISA Kit for Bovine HBGF-1

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E0032h

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96T

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ELISA Kit for Bovine HBGF-1

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E0032m

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96T

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ELISA Kit for Bovine HBGF-1

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E0032r

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ELISA Kit for Bovine HBGF-1

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E0032p

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ELISA Kit for Bovine HBGF-1

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E0032b

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ELISA Kit for Bovine HBGF-1

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E0032d

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ELISA Kit for Bovine HBGF-1

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E0032c

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96T

CLIA Kit for Bovine HBGF-1

Cat.:

U0032r

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CLIA Kit for Bovine HBGF-1

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U0032p

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CLIA Kit for Bovine HBGF-1

Cat.:

U0032m

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96T

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CLIA Kit for Bovine HBGF-1

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U0032h

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96T

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CLIA Kit for Bovine HBGF-1

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U0032d

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CLIA Kit for Bovine HBGF-1

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U0032b

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CLIA Kit for Bovine HBGF-1

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U0032c

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Polyclonal Antibody for Bovine HBGF-1

Cat.:

P0032Rb-r

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40ug/0.2ml

Polyclonal Antibody for Bovine HBGF-1

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P0032Rb-h

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40ug/0.2ml

Polyclonal Antibody for Bovine HBGF-1

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P0032Rb-m

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40ug/0.2ml

Polyclonal Antibody for Bovine HBGF-1

Monoclonal Antibody for Bovine HBGF-1

Protein for Bovine HBGF-1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.8%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of FGF1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	95	93-97
Plasma	97	96-102

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FGF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-100%	97-104%	95-98%	91-96%
EDTA plasma(n=5)	89-93%	102-105%	94-97%	93-98%
heparin plasma(n=5)	91-95%	96-99%	106-108%	87-93%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Nucleotide sequence of bovine acidic fibroblast growth factor cDNA."
Halley C. , Courtois Y. , Laurent M.
Nucleic Acids Res.16:10913-10913(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Retina.
2.

"Characterization of a bovine acidic FGF cDNA clone and its expression in brain and retina."
Alterio J. , Halley C. , Brou C. , Soussi T. , Courtois Y. , Laurent M.
FEBS Lett.242:41-46(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Retina.
3.

NIH - Mammalian Gene Collection (MGC) project
Submitted (2005-08) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain: Hereford.
tissue: Heart ventricle.
4.

"Structural evidence that endothelial cell growth factor beta is the precursor of both endothelial cell growth factor alpha and acidic fibroblast growth factor."
Burgess W.H. , Mehlman T. , Marshak D.R. , Fraser B.A. , Maciag T.
Proc. Natl. Acad. Sci. U.S.A.83:7216-7220(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-155;ACETYLATION AT ALA-2
5.

"Complete primary structure of prostatropin, a prostate epithelial cell growth factor."
Crabb J.W. , Armes L.G. , Carr S.A. , Johnson C.M. , Roberts G.D. , Bordoli R.S. , McKeehan W.L.
Biochemistry25:4988-4993(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-155
6.

"Brain-derived acidic fibroblast growth factor: complete amino acid sequence and homologies."
Gimenez-Gallego G. , Rodkey J. , Bennett C. , Rios-Candelore M. , Disalvo J. , Thomas K.
Science230:1385-1388(1985) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 16-155
7.

"Acidic fibroblast growth factor (FGF) from bovine brain: amino-terminal sequence and comparison with basic FGF."
Boehlen P. , Esch F. , Baird A. , Gospodarowicz D.
EMBO J.4:1951-1956(1985) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 16-44;AMINO-ACID COMPOSITION
8.

"Nucleotide sequence of a bovine clone encoding the angiogenic protein, basic fibroblast growth factor."
Abraham J.A. , Mergia A. , Whang J.L. , Tumolo A. , Friedman J. , Hjerrild K.A. , Gospodarowicz D. , Fiddes J.C.
Science233:545-548(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 16-56
9.

"Isolation of heparin-binding growth factors from bovine, porcine and canine hearts."
Quinkler W. , Maasberg M. , Bernotat-Danielowski S. , Luethe N. , Sharma H.S. , Schaper W.
Eur. J. Biochem.181:67-73(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 16-45
10.

"Cloning of two different 5' untranslated exons of bovine acidic fibroblast growth factor by the single strand ligation to single-stranded cDNA methodology."
Philippe J.-M. , Renaud F. , Desset S. , Laurent M. , Mallet J. , Courtois Y. , Edwards J.B.
Biochem. Biophys. Res. Commun.188:843-850(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-18
11.

"The extravesicular domain of synaptotagmin-1 is released with the latent fibroblast growth factor-1 homodimer in response to heat shock."
Tarantini F. , LaVallee T. , Jackson A. , Gamble S. , Mouta Carreira C. , Garfinkel S. , Burgess W.H. , Maciag T.
J. Biol. Chem.273:22209-22216(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SYT1;FUNCTION;HEPARIN BINDING;SUBCELLULAR LOCATION
12.

"Regulation of proliferation-survival decisions is controlled by FGF1 secretion in retinal pigmented epithelial cells."
Bryckaert M. , Guillonneau X. , Hecquet C. , Perani P. , Courtois Y. , Mascarelli F.
Oncogene19:4917-4929(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION
13.

"Three-dimensional structures of acidic and basic fibroblast growth factors."
Zhu X. , Komiya H. , Chirino A. , Faham S. , Fox G.M. , Arakawa T. , Hsu B.T. , Rees D.C.
Science251:90-93(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS)

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