Gdnf ELISA Kit for Rat(Rattus norvegicus) | EIAab.comGdnf ELISA Kit for Rat(Rattus norvegicus) | EIAab.com

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Gdnf (Gene name),

Glial cell line-derived neurotrophic factor (Protein name ), GDNF_RAT from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

Gdnf;

Protein name:

Glial cell line-derived neurotrophic factor;

Alternative:

Astrocyte-derived trophic factor(ATF);

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

Homodimer; disulfide-linked.

Function:

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. May also modulate local neuronal effects in distal regions of the motor neuron.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

211

Sequence:

50:

MKLWDVVAVC | LVLLHTASAF | PLPAGKRLLE | APAEDHSLGH | RRVPFALTSD |

100:

SNMPEDYPDQ | FDDVMDFIQA | TIKRLKRSPD | KQAAALPRRE | RNRQAAAASP |

150:

ENSRGKGRRG | QRGKNRGCVL | TAIHLNVTDL | GLGYETKEEL | IFRYCSGSCE |

200:

AAETMYDKIL | KNLSRSRRLT | SDKVGQACCR | PVAFDDDLSF | LDDSLVYHIL |

211:

RKHSAKRCGC | I

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

KEGG:

UniGene:

String:

SMR:

Uniprot:

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ELISA Kit for Rat Glial cell line-derived neurotrophic factor

Cat.:

E0043r

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MSDS:

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Packing:

96T

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ELISA Kit for Rat Glial cell line-derived neurotrophic factor

Cat.:

E0043h

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Packing:

96T

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ELISA Kit for Rat Glial cell line-derived neurotrophic factor

Cat.:

E0043m

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96T

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CLIA Kit for Rat Glial cell line-derived neurotrophic factor

Cat.:

U0043m

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Packing:

96T

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CLIA Kit for Rat Glial cell line-derived neurotrophic factor

Cat.:

U0043h

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Looking for manual.

MSDS:

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Packing:

96T

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CLIA Kit for Rat Glial cell line-derived neurotrophic factor

Cat.:

U0043r

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Manual:

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MSDS:

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Packing:

96T

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Polyclonal Antibody for Rat Glial cell line-derived neurotrophic factor

Cat.:

P0043Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat Glial cell line-derived neurotrophic factor

Cat.:

P0043Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat Glial cell line-derived neurotrophic factor

Cat.:

P0043Rb-r

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Rat Glial cell line-derived neurotrophic factor

Monoclonal Antibody for Rat Glial cell line-derived neurotrophic factor

Monoclonal Antibody for Rat Glial cell line-derived neurotrophic factor

Protein for Rat Glial cell line-derived neurotrophic factor

Protein for Rat Glial cell line-derived neurotrophic factor

Protein for Rat Glial cell line-derived neurotrophic factor

R&D Technical Data

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s

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Precision

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Recovery

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Recovery was determined by spiking various levels of GDNF into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	105	102-108
Plasma	106	99-107

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Linearity

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GDNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	102-106%	101-107%	96-103%	93-98%
EDTA plasma(n=5)	97-105%	104-108%	99-103%	89-96%
heparin plasma(n=5)	104-106%	107-110%	95-99%	100-105%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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References

1.

"GDNF: a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons."
Lin L.-F.H. , Doherty D.H. , Lile J.D. , Bektesh S. , Collins F.
Science260:1130-1132(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);HOMODIMERIZATION;DISULFIDE BONDS;GLYCOSYLATION;PROTEIN SEQUENCE OF 78-102
tissue: Glial tumor.
2.

"cDNA sequence and differential mRNA regulation of two forms of glial cell line-derived neurotrophic factor in Schwann cells and rat skeletal muscle."
Springer J.E. , Seeburger J.L. , He J. , Gabrea A. , Blankenhorn E.P. , Bergman L.W.
Exp. Neurol.131:47-52(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2)
3.

"Anterograde axonal transport of glial cell line-derived neurotrophic factor and its receptors in rat hypoglossal nerve."
Russell F.D. , Koishi K. , Jiang Y. , McLennan I.S.
Neuroscience97:575-580(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-125 (ISOFORMS 1; 2 AND 3);FUNCTION;TISSUE SPECIFICITY
strain: Wistar.
tissue: Brain.
4.

"GDNF is expressed in two forms in many tissues outside the CNS."
Suter-Crazzolara C. , Unsicker K.
NeuroReport5:2486-2488(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-76 (ISOFORM 2);ALTERNATIVE SPLICING;TISSUE SPECIFICITY
strain: Wistar.
tissue: Kidney.
5.

"The Rat GDNF gene."
Schmidt C. , Suter-Crazzolara C.
Submitted (1998-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50
6.

"Induction of GDNF mRNA expression by melatonin in rat C6 glioma cells."
Armstrong K.J. , Niles L.P.
NeuroReport13:473-475(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-207 (ISOFORM 1);INDUCTION
tissue: Glial tumor.
7.

"Multiple astrocyte transcripts encode nigral trophic factors in rat and human."
Schaar D.G. , Sieber B.-A. , Sherwood A.C. , Dean D. , Mendoza G. , Ramakrishnan L. , Dreyfus C.F. , Black I.B.
Exp. Neurol.130:387-393(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ALTERNATIVE SPLICING;TISSUE SPECIFICITY
8.

"X-ray structure of glial cell-derived neurotrophic factor at 1.9-A resolution and implications for receptor binding."
Eigenbrot C. , Gerber C.
Nat. Struct. Biol.4:435-438(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS)

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