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Index > Protein center > GPX1(Gene name) > Human
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  • GPX1 (Gene name),
  • Glutathione peroxidase 1 (Protein name ),  GPX1_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Gene name:
    GPX1;
    Protein name:
    Glutathione peroxidase 1(GPx-1;GSHPx-1);
    Alternative:
    Cellular glutathione peroxidase;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Homotetramer.
    Function:
    Protects the hemoglobin in erythrocytes from oxidative breakdown.
    Subcellular Location:
    Cytoplasm
    Protein Attributes:
    Sequence length:
    203
    Sequence:
    50:
    MCAARLAAAA | AAAQSVYAFS | ARPLAGGEPV | SLGSLRGKVL | LIENVASLUG | 
    100:
    TTVRDYTQMN | ELQRRLGPRG | LVVLGFPCNQ | FGHQENAKNE | EILNSLKYVR | 
    150:
    PGGGFEPNFM | LFEKCEVNGA | GAHPLFAFLR | EALPAPSDDA | TALMTDPKLI | 
    200:
    TWSPVCRNDV | AWNFEKFLVG | PDGVPLRRYS | RRFQTIDIEP | DIEALLSQGP | 
    203:
    SCA
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    SMR:
    MIM:
    UniGene:
    Pfam:
    KEGG:
    String:
    Uniprot:
     
    FOR
    ELISA Kit for Human GPx-1
    Cat.:
    E0295r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human GPx-1
    Cat.:
    E0295b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human GPx-1
    Cat.:
    E0295h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human GPx-1
    Cat.:
    E0295m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human GPx-1
    Cat.:
    E0295p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human GPx-1
    MSDS:
    Please sign in first.
    CLIA Kit for Human GPx-1
    Cat.:
    U0295h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human GPx-1
    MSDS:
    Please sign in first.
    CLIA Kit for Human GPx-1
    Cat.:
    U0295b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human GPx-1
    Cat.:
    U0295r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human GPx-1
    Cat.:
    U0295m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human GPx-1
    Cat.:
    U0295p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human GPx-1
    Cat.:
    P0295Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human GPx-1
    Polyclonal Antibody for Human GPx-1
    Polyclonal Antibody for Human GPx-1
    Cat.:
    P0295Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human GPx-1
    Polyclonal Antibody for Human GPx-1
    Cat.:
    P0295Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human GPx-1
    Monoclonal Antibody for Human GPx-1
    Monoclonal Antibody for Human GPx-1
    Monoclonal Antibody for Human GPx-1
    Monoclonal Antibody for Human GPx-1
    Monoclonal Antibody for Human GPx-1
    Protein for Human GPx-1
    Protein for Human GPx-1
    Protein for Human GPx-1
    Protein for Human GPx-1
    Protein for Human GPx-1
    Protein for Human GPx-1

    R&D Technical Data

    s

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    s

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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤8.3%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤10.8%

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    Recovery

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Recovery was determined by spiking various levels of GPX1 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    93-105

    Plasma

    101

    95-107

     

     

     

     

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    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GPX1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    93-105%

    95-102%

    92-108%

    90-110%

    EDTA plasma(n=5)

    91-110%

    93-107%

    94-108%

    97-105%

    heparin plasma(n=5)

    96-106%

     

    96-104%

    95-106%

    93-109%

     

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    References
    1. 1.
      "cDNA sequence coding for human glutathione peroxidase."
      Sukenaga Y. , Ishida K. , Takeda T. , Takagi K.
      Nucleic Acids Res.15:7178-7178(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);VARIANT 7-ALA-ALA-8 DEL
    2. 2.
      "Nucleotide sequence of a human gene for glutathione peroxidase."
      Ishida K. , Morino T. , Takagi K. , Sukenaga Y.
      Nucleic Acids Res.15:10051-10051(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1);VARIANT 7-ALA-ALA-8 DEL
    3. 3.
      "Sequence of a cDNA coding for human glutathione peroxidase confirms TGA encodes active site selenocysteine."
      Mullenbach G.T. , Tabrizi A. , Irvine B.D. , Bell G.I. , Hallewell R.A.
      Nucleic Acids Res.15:5484-5484(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);VARIANT 7-ALA-ALA-8 DEL
      tissue: Kidney.
    4. 4.
      "Isolation and chromosomal localization of the human glutathione peroxidase gene."
      Chada S. , le Beau M.M. , Casey L. , Newburger P.E.
      Genomics6:268-271(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);VARIANT 7-ALA-ALA-8 DEL
    5. 5.
      "Structure and function of the 5'-flanking sequence of the human cytosolic selenium-dependent glutathione peroxidase gene (hgpx1)."
      Moscow J.A. , Morrow C.S. , He R. , Mullenbach G.T. , Cowan K.H.
      J. Biol. Chem.267:5949-5958(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1);VARIANT ALA-8 DEL
    6. 6.
      NIEHS SNPs program
      Submitted (2003-06) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1);VARIANTS PRO-5; THR-194 AND LEU-200
    7. 7.
      "The DNA sequence, annotation and analysis of human chromosome 3."
      Muzny D.M. , Scherer S.E. , Kaul R. , Wang J. , Yu J. , Sudbrak R. , Buhay C.J. , Chen R. , Cree A. , Ding Y. , Dugan-Rocha S. , Gill R. , Gunaratne P. , Harris R.A. , Hawes A.C. , Hernandez J. , Hodgson A.V. , Hume J. , more...
      Nature440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    8. 8.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      tissue: Placenta.
    9. 9.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    10. 10.
      "Crystal structure of the selenocysteine to glycine mutant of human glutathione peroxidase 1."
      Structural genomics consortium (SGC)
      Submitted (2009-02) to the PDB data bank
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 14-198
    11. 11.
      "Low yield of polymorphisms from EST blast searching: analysis of genes related to oxidative stress and verification of the P197L polymorphism in GPX1."
      Forsberg L. , de Faire U. , Morgenstern R.
      Hum. Mutat.13:294-300(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT LEU-200
    12. 12.
      "Association between the GCG polymorphism of the selenium dependent GPX1 gene and the risk of young onset prostate cancer."
      Kote-Jarai Z. , Durocher F. , Edwards S.M. , Hamoudi R. , Jackson R.A. , Ardern-Jones A. , Murkin A. , Dearnaley D.P. , Kirby R. , Houlston R. , Easton D.F. , Eeles R.
      Prostate Cancer Prostatic Dis.5:189-192(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS ALA-8 DEL AND 7-ALA-ALA-8 DEL
    13. 13.
      "Functional variants in the glutathione peroxidase-1 (GPx-1) gene are associated with increased intima-media thickness of carotid arteries and risk of macrovascular diseases in Japanese type 2 diabetic patients."
      Hamanishi T. , Furuta H. , Kato H. , Doi A. , Tamai M. , Shimomura H. , Sakagashira S. , Nishi M. , Sasaki H. , Sanke T. , Nanjo K.
      Diabetes53:2455-2460(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS ALA-8 DEL AND LEU-200
    14. 14.
      "Increased risk of bladder cancer associated with a glutathione peroxidase 1 codon 198 variant."
      Ichimura Y. , Habuchi T. , Tsuchiya N. , Wang L. , Oyama C. , Sato K. , Nishiyama H. , Ogawa O. , Kato T.
      J. Urol.172:728-732(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT LEU-200
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