Gpx1 ELISA Kit for Rat(Rattus norvegicus) | EIAab.comGpx1 ELISA Kit for Rat(Rattus norvegicus) | EIAab.com

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Gpx1 (Gene name),

Glutathione peroxidase 1 (Protein name ), GPX1_RAT from NCBI database.

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General Annotation

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Antigen Annotation

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3D

|

Predicted Eptitope

|

Vaild Sequence

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Related Databases

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Citations (2)

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Gene name:

Gpx1;

Protein name:

Glutathione peroxidase 1(GPx-1;GSHPx-1);

Alternative:

Cellular glutathione peroxidase;

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

Homotetramer.

Function:

Protects the hemoglobin in erythrocytes from oxidative breakdown.

Subcellular Location:

Cytoplasm

Protein Attributes:

Sequence length:

201

Sequence:

50:

MSAARLSAVA | QSTVYAFSAR | PLAGGEPVSL | GSLRGKVLLI | ENVASLUGTT |

100:

TRDYTEMNDL | QKRLGPRGLV | VLGFPCNQFG | HQENGKNEEI | LNSLKYVRPG |

150:

GGFEPNFTLF | EKCEVNGEKA | HPLFTFLRNA | LPAPSDDPTA | LMTDPKYIIW |

200:

SPVCRNDISW | NFEKFLVGPD | GVPVRRYSRR | FRTIDIEPDI | EALLSKQPSN |

201:

P

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

UniGene:

Pfam:

Uniprot:

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ELISA Kit for Rat GPx-1

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E0295r

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96T

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ELISA Kit for Rat GPx-1

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E0295b

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96T

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ELISA Kit for Rat GPx-1

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E0295h

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ELISA Kit for Rat GPx-1

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E0295m

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ELISA Kit for Rat GPx-1

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E0295p

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ELISA Kit for Rat GPx-1

MSDS:

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CLIA Kit for Rat GPx-1

Cat.:

U0295h

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96T

CLIA Kit for Rat GPx-1

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CLIA Kit for Rat GPx-1

Cat.:

U0295b

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96T

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CLIA Kit for Rat GPx-1

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U0295r

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Looking for manual.

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96T

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CLIA Kit for Rat GPx-1

Cat.:

U0295m

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96T

CLIA Kit for Rat GPx-1

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U0295p

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96T

Polyclonal Antibody for Rat GPx-1

Cat.:

P0295Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat GPx-1

Polyclonal Antibody for Rat GPx-1

Polyclonal Antibody for Rat GPx-1

Cat.:

P0295Rb-m

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40ug/0.2ml

Polyclonal Antibody for Rat GPx-1

Polyclonal Antibody for Rat GPx-1

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P0295Rb-r

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40ug/0.2ml

Monoclonal Antibody for Rat GPx-1

Monoclonal Antibody for Rat GPx-1

Monoclonal Antibody for Rat GPx-1

Monoclonal Antibody for Rat GPx-1

Monoclonal Antibody for Rat GPx-1

Monoclonal Antibody for Rat GPx-1

Protein for Rat GPx-1

Protein for Rat GPx-1

Protein for Rat GPx-1

Protein for Rat GPx-1

Protein for Rat GPx-1

Protein for Rat GPx-1

R&D Technical Data

s

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s

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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤8.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.8%

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Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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Recovery was determined by spiking various levels of GPX1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	93-105
Plasma	101	95-107

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Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GPX1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-105%	95-102%	92-108%	90-110%
EDTA plasma(n=5)	91-110%	93-107%	94-108%	97-105%
heparin plasma(n=5)	96-106%	96-104%	95-106%	93-109%

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Citations

[1].

"Therapeutic effectiveness of rectally administered fish oil and mesalazine in trinitrobenzenesulfonic acid-induced colitis"

E Yorulmaz, H Yorulmaz, O Zengi, et al.

From:Biomedicine & Pharmacotherapy

[2].

"Intraperitoneal Nigella sativa for Prevention of Postoperative Intra-Abdominal Adhesions in Rats"

A Karatas, T Ozlu, G Ozyalvacli, et al.

From:Journal of Investigative Surgery

References

1.

"Determination of nucleotide sequence of cDNA coding rat glutathione peroxidase and diminished expression of the mRNA in selenium deficient rat liver."
Yoshimura S. , Takekoshi S. , Watanabe K. , Fujii-Kuriyama Y.
Biochem. Biophys. Res. Commun.154:1024-1028(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
2.

"Nucleotide sequence of a rat glutathione peroxidase cDNA."
Ho Y.S. , Howard A.J. , Crapo J.D.
Nucleic Acids Res.16:5207-5207(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Sprague-Dawley.
tissue: Liver.
3.

"Expression of glutathione peroxidase I gene in selenium-deficient rats."
Reddy A.P. , Hsu B.L. , Reddy P.S. , Li N.Q. , Thyagaraju K. , Reddy C.C. , Tam M.F. , Tu C.P.D.
Nucleic Acids Res.16:5557-5568(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
4.

Reddy A.P. , Hsu B.L. , Reddy P.S. , Li N.Q. , Thyagaraju K. , Reddy C.C. , Tam M.F. , Tu C.P.D.
Submitted (2010-12) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SEQUENCE REVISION TO 47
5.

"Cloning and characterization of the rat glutathione peroxidase gene."
Ho Y.-S. , Howard A.J.
FEBS Lett.301:5-9(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA];TISSUE SPECIFICITY
strain: Sprague-Dawley.
6.

"Dietary selenium stabilizes glutathione peroxidase mRNA in rat liver."
Christensen M.J. , Burgener K.W.
J. Nutr.122:1620-1626(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Sprague-Dawley.
tissue: Liver.
7.

"Functional analysis of the 5'-flanking region of the rat glutathione peroxidase gene."
Suemizu H.
Submitted (1997-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-82
strain: Sprague-Dawley.
8.

"Amino acid sequence around the active-site selenocysteine of rat liver glutathione peroxidase."
Condell R.A. , Tappel A.L.
Biochim. Biophys. Acta709:304-309(1982) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 7-52;SELENOCYSTEINE AT SEC-47
tissue: Liver.
9.

Lubec G. , Afjehi-Sadat L.
Submitted (2006-11) to the UniProtKB

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 96-112 AND 165-176;IDENTIFICATION BY MASS SPECTROMETRY
strain: Sprague-Dawley.
tissue: Spinal cord.

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