GPX3 ELISA Kit for Human(Homo sapiens) | EIAab.comGPX3 ELISA Kit for Human(Homo sapiens)

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Index > Protein center > GPX3(Gene name) > Human

GPX3 (Gene name),

Glutathione peroxidase 3 (Protein name ), GPX3_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (1)

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Gene name:

GPX3(GPXP);

Protein name:

Glutathione peroxidase 3(GPx-3;GSHPx-3);

Alternative:

Plasma glutathione peroxidase(GPx-P;GSHPx-P);Extracellular glutathione peroxidase;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homotetramer.

Function:

Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

226

Sequence:

50:

100:

150:

200:

226:

RTTVSNVKMD | ILSYMRRQAA | LGVKRK

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

MIM:

138321

String:

P22352

KEGG:

hsa:2878

UniGene:

Hs.386793

SMR:

P22352

Pfam:

PF00255

STRING:

9606.ENSP00000373477

Uniprot:

P22352

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ELISA Kit for Human GPx-3

Cat.:

E9949h

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96T

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ELISA Kit for Human GPx-3

Cat.:

E9949m

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96T

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ELISA Kit for Human GPx-3

Cat.:

E9949b

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ELISA Kit for Human GPx-3

Cat.:

E9949r

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96T

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CLIA Kit for Human GPx-3

Cat.:

U9949h

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96T

CLIA Kit for Human GPx-3

Cat.:

U9949m

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96T

CLIA Kit for Human GPx-3

Cat.:

U9949r

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96T

CLIA Kit for Human GPx-3

Cat.:

U9949b

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96T

Polyclonal Antibody for Human GPx-3

Cat.:

P9949Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human GPx-3

Cat.:

P9949Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human GPx-3

Monoclonal Antibody for Human GPx-3

Protein for Human GPx-3

R&D Technical Data

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 5.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.8%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	91-104
Plasma	95	92-103

Recovery was determined by spiking various levels of Gpx3 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	92-103
Plasma	98	93-105

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-101%	92-103%	92-105%	94-106%
EDTA plasma(n=5)	89-97%	93-102%	92-98%	90-103%
heparin plasma(n=5)	93-101%	94-104%	101-108%	91-104%

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gpx3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-103%	92-104%	90-105%	92-102%
EDTA plasma(n=5)	90-106%	93-105%	92-104%	91-105%
heparin plasma(n=5)	95-103%	94-103%	91-107%	94-101%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"Alteration in the intrafollicular thiol–redox system in infertile women with endometriosis"

YS Choi, SH Cho, SK Seo, et al.

From:Reproduction

References

1.

"Primary structure of human plasma glutathione peroxidase deduced from cDNA sequences."
Takahashi K. , Akasaka M. , Yamamoto Y. , Kobayashi C. , Mizoguchi J. , Koyama J.
J. Biochem.108:145-148(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PARTIAL PROTEIN SEQUENCE
tissue: Fetal liver.
tissue: Placenta.
2.

"Expression of plasma glutathione peroxidase in human liver in addition to kidney, heart, lung, and breast in humans and rodents."
Chu F.-F. , Esworthy R.S. , Doroshow J.H. , Doan K. , Liu X.F.
Blood79:3233-3238(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Kidney.
3.

Chu F.-F.
Submitted (2010-11) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SEQUENCE REVISION TO 73
4.

"The human plasma glutathione peroxidase-encoding gene: organization, sequence and localization to chromosome 5q32."
Yoshimura S. , Suemizu H. , Taniguchi Y. , Arimori K. , Kawabe N. , Moriuchi T.
Gene145:293-297(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
tissue: Lymphocyte.
5.

"Differential induction of extracellular glutathione peroxidase and nitric oxide synthase 2 in airways of healthy individuals exposed to 100% O(2) or cigarette smoke."
Comhair S.A.A. , Thomassen M.J. , Erzurum S.C.
Am. J. Respir. Cell Mol. Biol.23:350-354(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
6.

NIEHS SNPs program
Submitted (2003-05) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT LEU-128
7.

"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M. , Scherer S.E. , Kaul R. , Wang J. , Yu J. , Sudbrak R. , Buhay C.J. , Chen R. , Cree A. , Ding Y. , Dugan-Rocha S. , Gill R. , Gunaratne P. , Harris R.A. , Hawes A.C. , Hernandez J. , Hodgson A.V. , Hume J. , more...
Nature440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
8.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Duodenum.
tissue: Lung.
tissue: Pancreas.
9.

"Characterization and partial amino acid sequence of human plasma glutathione peroxidase."
Esworthy R.S. , Chu F.-F. , Paxton R.J. , Akman S. , Doroshow J.H.
Arch. Biochem. Biophys.286:330-336(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 107-138 AND 148-175;CHARACTERIZATION
10.

"Crystal structure of human glutathione peroxidase 3 (selenocysteine to glycine mutant)."
Structural genomics consortium (SGC)
Submitted (2007-09) to the PDB data bank

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 25-223

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