Grb2 ELISA Kit for Mouse(Mus musculus) | EIAab.comGrb2 ELISA Kit for Mouse(Mus musculus)

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Grb2 (Gene name),

Growth factor receptor-bound protein 2 (Protein name ), GRB2_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Grb2;

Protein name:

Growth factor receptor-bound protein 2;

Alternative:

SH2/SH3 adapter GRB2;Adapter protein GRB2;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Associates with activated Tyr-phosphorylated EGF receptors and PDGF receptors via its SH2 domain. Also associates to other cellular Tyr-phosphorylated proteins such as SIT1, IRS1, IRS4, SHC and LNK; probably via the concerted action of both its SH2 and SH3 domains. It also seems to interact with RAS in the signaling pathway leading to DNA synthesis. Binds to and translocates the guanine nucleotide exchange factors SOS. Interacts with phosphorylated TOM1L1 and MET. Interacts with the phosphorylated C-terminus of SH2B2. Interacts with phosphorylated SIT1, LAX1, LAT, LAT2 and LIME1 upon TCR and/or BCR activation. Interacts with PTPNS1, REPS2 and the syntrophin SNTA1. Interacts with REPS1 and PIK3C2B via its SH3 domains. Interacts with CBL and CBLB. Interacts with JUB. Interacts with SHB, INPP5D/SHIP1, SKAP1 and SKAP2 (By similarity). Interacts with CLNK. Forms a complex with MUC1 and SOS1, through interaction of the SH3 domains with SOS1 and the SH2 domain with phosphorylated MUC1. Interacts with PRNP (By similarity). Interacts with NISCH and RALGPS1 (By similarity). Interacts also with HCST. Interacts with GAPT and PTPRE. Interacts (via SH2 domain) with KIF26A (By similarity). Interacts with THEMIS. Interacts (via SH3 2) with GAB2. Interacts with ADAM15. Interacts with PTPRJ and BCR (By similarity). Interacts with THEMIS2. Interacts (via SH2 domain) with AXL and KIT (phosphorylated). Interacts with EPHB1 and SHC1; activates the MAPK/ERK cascade to regulate cell migration.

Function:

Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.

Subcellular Location:

Golgi apparatus

Protein Attributes:

Sequence length:

217

Sequence:

50:

100:

150:

200:

217:

QTGMFPRNYV | TPVNRNV

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

Q60631

Pfam:

PF00017

UniGene:

Mm.439649

SMR:

Q60631

KEGG:

mmu:14784

Uniprot:

Q60631

FOR

ELISA Kit for Mouse Growth factor receptor-bound protein 2

CLIA Kit for Mouse Growth factor receptor-bound protein 2

Polyclonal Antibody for Mouse Growth factor receptor-bound protein 2

Monoclonal Antibody for Mouse Growth factor receptor-bound protein 2

Protein for Mouse Growth factor receptor-bound protein 2

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Molecular cloning of the mouse grb2 gene: differential interaction of the Grb2 adaptor protein with epidermal growth factor and nerve growth factor receptors."
Suen K. , Bustelo X.R. , Pawson T. , Barbacid M.
Mol. Cell. Biol.13:5500-5512(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: BALB/c.
2.

Tanaka S.
Submitted (1996-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)
strain: BALB/c.
3.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
strain: C57BL/6.
strain: FVB/N.
tissue: Brain.
tissue: Mammary gland.
4.

Lubec G. , Klug S.
Submitted (2007-03) to the UniProtKB

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 125-136;IDENTIFICATION BY MASS SPECTROMETRY
tissue: Hippocampus.
5.

"Integrin-mediated signal transduction linked to Ras pathway by GRB2 binding to focal adhesion kinase."
Schlaepfer D.D. , Hanks S.K. , Hunter T. , van der Geer P.
Nature372:786-791(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PTK2/FAK1
6.

"Phosphorylation of tyrosine 720 in the platelet-derived growth factor alpha receptor is required for binding of Grb2 and SHP-2 but not for activation of Ras or cell proliferation."
Bazenet C.E. , Gelderloos J.A. , Kazlauskas A.
Mol. Cell. Biol.16:6926-6936(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PDGFRA AND PTPN11
7.

"Sequential activation of phoshatidylinositol 3-kinase and phospholipase C-gamma2 by the M-CSF receptor is necessary for differentiation signaling."
Bourette R.P. , Myles G.M. , Choi J.L. , Rohrschneider L.R.
EMBO J.16:5880-5893(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CSF1R;PHOSPHORYLATION
8.

"An eps homology (EH) domain protein that binds to the ral-GTPase target, RalBP1."
Yamaguchi A. , Urano T. , Goi T. , Feig L.A.
J. Biol. Chem.272:31230-31234(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH REPS1
tissue: Muscle.
9.

"Identification of vascular endothelial growth factor receptor-1 tyrosine phosphorylation sites and binding of SH2 domain-containing molecules."
Ito N. , Wernstedt C. , Engstrom U. , Claesson-Welsh L.
J. Biol. Chem.273:23410-23418(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH FLT1
10.

"Disabled-2 (Dab2) is an SH3 domain-binding partner of Grb2."
Xu X.X. , Yi T. , Tang B. , Lambeth J.D.
Oncogene16:1561-1569(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH DAB2
11.

"Identification of Tyr-703 and Tyr-936 as the primary association sites for Grb2 and Grb7 in the c-Kit/stem cell factor receptor."
Thommes K. , Lennartsson J. , Carlberg M. , Ronnstrand L.
Biochem. J.341:211-216(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH KIT
12.

"Gab-family adapter proteins act downstream of cytokine and growth factor receptors and T- and B-cell antigen receptors."
Nishida K. , Yoshida Y. , Itoh M. , Fukada T. , Ohtani T. , Shirogane T. , Atsumi T. , Takahashi-Tezuka M. , Ishihara K. , Hibi M. , Hirano T.
Blood93:1809-1816(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH GAB2
13.

"Identification of Tek/Tie2 binding partners. Binding to a multifunctional docking site mediates cell survival and migration."
Jones N. , Master Z. , Jones J. , Bouchard D. , Gunji Y. , Sasaki H. , Daly R. , Alitalo K. , Dumont D.J.
J. Biol. Chem.274:30896-30905(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TEK/TIE2
14.

"Ajuba, a novel LIM protein, interacts with Grb2, augments mitogen-activated protein kinase activity in fibroblasts, and promotes meiotic maturation of Xenopus oocytes in a Grb2- and Ras-dependent manner."
Goyal R.K. , Lin P. , Kanungo J. , Payne A.S. , Muslin A.J. , Longmore G.D.
Mol. Cell. Biol.19:4379-4389(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH AJUBA
15.

"Mouse alpha1-syntrophin binding to Grb2: further evidence of a role for syntrophin in cell signaling."
Oak S.A. , Russo K. , Petrucci T.C. , Jarrett H.W.
Biochemistry40:11270-11278(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SNTA1
16.

"MIST functions through distinct domains in immunoreceptor signaling in the presence and absence of LAT."
Goitsuka R. , Tatsuno A. , Ishiai M. , Kurosaki T. , Kitamura D.
J. Biol. Chem.276:36043-36050(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CLNK
17.

"PrPC directly interacts with proteins involved in signaling pathways."
Spielhaupter C. , Schaetzl H.M.
J. Biol. Chem.276:44604-44612(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;INTERACTION WITH PRNP
18.

"Insulin receptor substrate 3 (IRS-3) and IRS-4 impair IRS-1- and IRS-2-mediated signaling."
Tsuruzoe K. , Emkey R. , Kriauciunas K.M. , Ueki K. , Kahn C.R.
Mol. Cell. Biol.21:26-38(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH IRS4
19.

"'Srcasm: a novel Src activating and signaling molecule."
Seykora J.T. , Mei L. , Dotto G.P. , Stein P.L.
J. Biol. Chem.277:2812-2822(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TOM1L1
20.

"EphB1 recruits c-Src and p52Shc to activate MAPK/ERK and promote chemotaxis."
Vindis C. , Cerretti D.P. , Daniel T.O. , Huynh-Do U.
J. Cell Biol.162:661-671(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH EPHB1 AND SHC1
21.

"LIME, a novel transmembrane adaptor protein, associates with p56lck and mediates T cell activation."
Hur E.M. , Son M. , Lee O.-H. , Choi Y.B. , Park C. , Lee H. , Yun Y.
J. Exp. Med.198:1463-1473(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH LIME1
22.

"Positive and negative regulation of FcepsilonRI-mediated signaling by the adaptor protein LAB/NTAL."
Zhu M. , Liu Y. , Koonpaew S. , Granillo O. , Zhang W.
J. Exp. Med.200:991-1000(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH LAT2
23.

"Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL."
Volna P. , Lebduska P. , Draberova L. , Simova S. , Heneberg P. , Boubelik M. , Bugajev V. , Malissen B. , Wilson B.S. , Horejsi V. , Malissen M. , Draber P.
J. Exp. Med.200:1001-1013(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH LAT2
24.

"LIME acts as a transmembrane adapter mediating BCR-dependent B-cell activation."
Ahn E. , Lee H. , Yun Y.
Blood107:1521-1527(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH LIME1
25.

"Phosphorylation of Tyr1214 within VEGFR-2 triggers the recruitment of Nck and activation of Fyn leading to SAPK2/p38 activation and endothelial cell migration in response to VEGF."
Lamalice L. , Houle F. , Huot J.
J. Biol. Chem.281:34009-34020(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH KDR
26.

"NKG2D-mediated signaling requires a DAP10-bound Grb2-Vav1 intermediate and phosphatidylinositol-3-kinase in human natural killer cells."
Upshaw J.L. , Arneson L.N. , Schoon R.A. , Dick C.J. , Billadeau D.D. , Leibson P.J.
Nat. Immunol.7:524-532(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HCST
27.

"Themis, a T cell-specific protein important for late thymocyte development."
Lesourne R. , Uehara S. , Lee J. , Song K.-D. , Li L. , Pinkhasov J. , Zhang Y. , Weng N.-P. , Wildt K.F. , Wang L. , Bosselut R. , Love P.E.
Nat. Immunol.10:840-847(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH THEMIS
28.

"Themis is a member of a new metazoan gene family and is required for the completion of thymocyte positive selection."
Johnson A.L. , Aravind L. , Shulzhenko N. , Morgun A. , Choi S.-Y. , Crockford T.L. , Lambe T. , Domaschenz H. , Kucharska E.M. , Zheng L. , Vinuesa C.G. , Lenardo M.J. , Goodnow C.C. , Cornall R.J. , Schwartz R.H.
Nat. Immunol.10:831-839(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH THEMIS
29.

"Gasp, a Grb2-associating protein, is critical for positive selection of thymocytes."
Patrick M.S. , Oda H. , Hayakawa K. , Sato Y. , Eshima K. , Kirikae T. , Iemura S. , Shirai M. , Abe T. , Natsume T. , Sasazuki T. , Suzuki H.
Proc. Natl. Acad. Sci. U.S.A.106:16345-16350(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH THEMIS
30.

"Themis2/ICB1 is a signaling scaffold that selectively regulates macrophage Toll-like receptor signaling and cytokine production."
Peirce M.J. , Brook M. , Morrice N. , Snelgrove R. , Begum S. , Lanfrancotti A. , Notley C. , Hussell T. , Cope A.P. , Wait R.
PLoS ONE5:E11465-E11465(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH THEMIS2
31.

"Rab13-dependent trafficking of RhoA is required for directional migration and angiogenesis."
Wu C. , Agrawal S. , Vasanji A. , Drazba J. , Sarkaria S. , Xie J. , Welch C.M. , Liu M. , Anand-Apte B. , Horowitz A.
J. Biol. Chem.286:23511-23520(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH RAB13
32.

"SCIMP, a transmembrane adapter protein involved in major histocompatibility complex class II signaling."
Draber P. , Vonkova I. , Stepanek O. , Hrdinka M. , Kucova M. , Skopcova T. , Otahal P. , Angelisova P. , Horejsi V. , Yeung M. , Weiss A. , Brdicka T.
Mol. Cell. Biol.31:4550-4562(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SCIMP
33.

"Tespa1 is involved in late thymocyte development through the regulation of TCR-mediated signaling."
Wang D. , Zheng M. , Lei L. , Ji J. , Yao Y. , Qiu Y. , Ma L. , Lou J. , Ouyang C. , Zhang X. , He Y. , Chi J. , Wang L. , Kuang Y. , Wang J. , Cao X. , Lu L.
Nat. Immunol.13:560-568(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH LAT; PLCG1 AND THEMIS
tissue: Thymocyte.
34.

"Solution structure of the Grb2 N-terminal SH3 domain complexed with a ten-residue peptide derived from SOS: direct refinement against NOEs, J-couplings and 1H and 13C chemical shifts."
Wittekind M. , Mapelli C. , Lee V. , Goldfarb V. , Friedrichs M.S. , Meyers C.A. , Mueller L.
J. Mol. Biol.267:933-952(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 1-65

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