Gene name:
H2AFY (MACROH2A1) ;
Protein name:
Core histone macro-H2A.1 (Histone macroH2A1;mH2A1) ;
Alternative:
Medulloblastoma antigen MU-MB-50.205 ;Histone H2A.y (H2A/y) ;
Organism:
Human (Homo sapiens).
General Annotation
Sub Unit:
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with HDAC1 and HDAC2.
Function:
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.
Subcellular Location:
Nucleus
Chromosome
Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.
Protein Attributes:
50:
MSSRGGKKKS | TKTSRSAKAG | VIFPVGRMLR | YIKKGHPKYR | IGVGAPVYMA |
100:
AVLEYLTAEI | LELAGNAARD | NKKGRVTPRH | ILLAVANDEE | LNQLLKGVTI |
150:
ASGGVLPNIH | PELLAKKRGS | KGKLEAIITP | PPAKKAKSPS | QKKPVSKKAG |
200:
GKKGARKSKK | KQGEVSKAAS | ADSTTEGTPA | DGFTVLSTKS | LFLGQKLNLI |
250:
HSEISNLAGF | EVEAIINPTN | ADIDLKDDLG | NTLEKKGGKE | FVEAVLELRK |
300:
KNGPLEVAGA | AVSAGHGLPA | KFVIHCNSPV | WGADKCEELL | EKTVKNCLAL |
350:
ADDKKLKSIA | FPSIGSGRNG | FPKQTAAQLI | LKAISSYFVS | TMSSSIKTVY |
372:
FVLFDSESIG | IYVQEMAKLD | AN
Vaild Sequence:
Related Databases
Uniprot:
ELISA Kit
CLIA Kit
Polyclonal Antibody
Monoclonal Antibody
Protein
FOR
Human
Chicken
Rat
Mouse
ELISA Kit for Human Histone macroH2A1
ELISA Kit for Human Histone macroH2A1
ELISA Kit for Human Histone macroH2A1
ELISA Kit for Human Histone macroH2A1
CLIA Kit for Human Histone macroH2A1
CLIA Kit for Human Histone macroH2A1
CLIA Kit for Human Histone macroH2A1
CLIA Kit for Human Histone macroH2A1
Polyclonal Antibody for Human Histone macroH2A1
Polyclonal Antibody for Human Histone macroH2A1
Polyclonal Antibody for Human Histone macroH2A1
Polyclonal Antibody for Human Histone macroH2A1
Monoclonal Antibody for Human Histone macroH2A1
Monoclonal Antibody for Human Histone macroH2A1
Monoclonal Antibody for Human Histone macroH2A1
Monoclonal Antibody for Human Histone macroH2A1
Protein for Human Histone macroH2A1
Protein for Human Histone macroH2A1
Protein for Human Histone macroH2A1
Protein for Human Histone macroH2A1
R&D Technical Data
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Precision
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Recovery
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Linearity
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
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Liver .
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
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Placenta .
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
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12.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
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13.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-129 AND THR-178;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
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Cervix carcinoma .
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
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16.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-129;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Cervix carcinoma .
17.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-170 AND THR-178;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Cervix carcinoma .
18.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
19.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-129; SER-170; SER-173 AND THR-178;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Leukemic T-cell .
20.
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Sci. Signal.3:RA3-RA3(2010)
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[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-129 AND THR-178;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Cervix carcinoma .
21.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-129 AND SER-170;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
22.
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Cited for : X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 1-120 IN COMPLEX WITH THE NUCLEOSOME CORE PARTICLE;FUNCTION
23.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : X-RAY CRYSTALLOGRAPHY (2.54 ANGSTROMS) OF 162-372 (ISOFORM 1);X-RAY CRYSTALLOGRAPHY (2.92 ANGSTROMS) OF 161-372 (ISOFORM 2);BINDING TO ADP-RIBOSE AND O-ACETYL-ADP-RIBOSE (ISOFORM 1)
24.
"Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases."
Zhuang M.
,
Calabrese M.F.
,
Liu J.
,
Waddell M.B.
,
Nourse A.
,
Hammel M.
,
Miller D.J.
,
Walden H.
,
Duda D.M.
,
Seyedin S.N.
,
Hoggard T.
,
Harper J.W.
,
White K.P.
,
Schulman B.A.
Mol. Cell36:39-50(2009)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
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Cited for : X-RAY CRYSTALLOGRAPHY (1.43 ANGSTROMS) OF 172-186 IN COMPLEXES WITH SPOP;SUBUNIT;UBIQUITINATION