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Index > Protein center > H2AFY(Gene name) > Human
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  • H2AFY (Gene name),
  • Core histone macro-H2A.1 (Protein name ),  H2AY_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    H2AFY(MACROH2A1);
    Protein name:
    Core histone macro-H2A.1(Histone macroH2A1;mH2A1);
    Alternative:
    Medulloblastoma antigen MU-MB-50.205;Histone H2A.y(H2A/y);
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with HDAC1 and HDAC2.
    Function:
    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.
    Subcellular Location:
    Nucleus Chromosome Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.
    Protein Attributes:
    Sequence length:
    372
    Sequence:
    50:
    MSSRGGKKKS | TKTSRSAKAG | VIFPVGRMLR | YIKKGHPKYR | IGVGAPVYMA | 
    100:
    AVLEYLTAEI | LELAGNAARD | NKKGRVTPRH | ILLAVANDEE | LNQLLKGVTI | 
    150:
    ASGGVLPNIH | PELLAKKRGS | KGKLEAIITP | PPAKKAKSPS | QKKPVSKKAG | 
    200:
    GKKGARKSKK | KQGEVSKAAS | ADSTTEGTPA | DGFTVLSTKS | LFLGQKLNLI | 
    250:
    HSEISNLAGF | EVEAIINPTN | ADIDLKDDLG | NTLEKKGGKE | FVEAVLELRK | 
    300:
    KNGPLEVAGA | AVSAGHGLPA | KFVIHCNSPV | WGADKCEELL | EKTVKNCLAL | 
    350:
    ADDKKLKSIA | FPSIGSGRNG | FPKQTAAQLI | LKAISSYFVS | TMSSSIKTVY | 
    372:
    FVLFDSESIG | IYVQEMAKLD | AN
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    KEGG:
    Pfam:
    UniGene:
    String:
    MIM:
    SMR:
    Uniprot:
     
    FOR
    ELISA Kit for Human Histone macroH2A1
    ELISA Kit for Human Histone macroH2A1
    ELISA Kit for Human Histone macroH2A1
    ELISA Kit for Human Histone macroH2A1
    CLIA Kit for Human Histone macroH2A1
    CLIA Kit for Human Histone macroH2A1
    CLIA Kit for Human Histone macroH2A1
    CLIA Kit for Human Histone macroH2A1
    Polyclonal Antibody for Human Histone macroH2A1
    Polyclonal Antibody for Human Histone macroH2A1
    Polyclonal Antibody for Human Histone macroH2A1
    Polyclonal Antibody for Human Histone macroH2A1
    Monoclonal Antibody for Human Histone macroH2A1
    Monoclonal Antibody for Human Histone macroH2A1
    Monoclonal Antibody for Human Histone macroH2A1
    Monoclonal Antibody for Human Histone macroH2A1
    Protein for Human Histone macroH2A1
    Protein for Human Histone macroH2A1
    Protein for Human Histone macroH2A1
    Protein for Human Histone macroH2A1

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    References
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      "Isolation of cDNA clones encoding human histone macroH2A1 subtypes."
      Lee Y. , Hong M. , Kim J.W. , Hong Y.M. , Choe Y.-K. , Chang S.Y. , Lee K.S. , Choe I.S.
      Biochim. Biophys. Acta1399:73-77(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2);TISSUE SPECIFICITY
      tissue: Liver.
    2. 2.
      "Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning."
      Mao M. , Fu G. , Wu J.-S. , Zhang Q.-H. , Zhou J. , Kan L.-X. , Huang Q.-H. , He K.-L. , Gu B.-W. , Han Z.-G. , Shen Y. , Gu J. , Yu Y.-P. , Xu S.-H. , Wang Y.-X. , Chen S.-J. , Chen Z.
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      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3)
      tissue: Umbilical cord blood.
    3. 3.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
      tissue: Ovary.
    4. 5.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
      tissue: Bone marrow.
      tissue: Placenta.
    5. 6.
      "Novel tumor antigens identified by autologous antibody screening of childhood medulloblastoma cDNA libraries."
      Behrends U. , Schneider I. , Roessler S. , Frauenknecht H. , Golbeck A. , Lechner B. , Eigenstetter G. , Zobywalski C. , Mueller-Weihrich S. , Graubner U. , Schmid I. , Sackerer D. , Spaeth M. , Goetz C. , Prantl F. , Asmuss H.-P. , Bise K. , Mautner J.
      Int. J. Cancer106:244-251(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-372 (ISOFORM 3)
      tissue: Medulloblastoma.
    6. 7.
      "Histone macroH2A1 is concentrated in the inactive X chromosome of female mammals."
      Costanzi C. , Pehrson J.R.
      Nature393:599-601(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION
    7. 8.
      "The histone variant macroH2A interferes with transcription factor binding and SWI/SNF nucleosome remodeling."
      Angelov D. , Molla A. , Perche P.-Y. , Hans F. , Cote J. , Khochbin S. , Bouvet P. , Dimitrov S.
      Mol. Cell11:1033-1041(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    8. 9.
      "Histone variant macroH2A1.2 is mono-ubiquitinated at its histone domain."
      Ogawa Y. , Ono T. , Wakata Y. , Okawa K. , Tagami H. , Shibahara K.
      Biochem. Biophys. Res. Commun.336:204-209(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION AT LYS-116 AND LYS-117;IDENTIFICATION BY MASS SPECTROMETRY
    9. 10.
      "Formation of MacroH2A-containing senescence-associated heterochromatin foci and senescence driven by ASF1a and HIRA."
      Zhang R. , Poustovoitov M.V. , Ye X. , Santos H.A. , Chen W. , Daganzo S.M. , Erzberger J.P. , Serebriiskii I.G. , Canutescu A.A. , Dunbrack R.L. , Pehrson J.R. , Berger J.M. , Kaufman P.D. , Adams P.D.
      Dev. Cell8:19-30(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION
    10. 11.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: BINDING TO ADP-RIBOSE (ISOFORM 1)
    11. 12.
      "Stable X chromosome inactivation involves the PRC1 Polycomb complex and requires histone MACROH2A1 and the CULLIN3/SPOP ubiquitin E3 ligase."
      Hernandez-Munoz I. , Lund A.H. , van der Stoop P. , Boutsma E. , Muijrers I. , Verhoeven E. , Nusinow D.A. , Panning B. , Marahrens Y. , van Lohuizen M.
      Proc. Natl. Acad. Sci. U.S.A.102:7635-7640(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION IN A COMPLEX WITH CULLIN3 AND SPOP;UBIQUITINATION;SUBCELLULAR LOCATION;FUNCTION
    12. 13.
      "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V. , Blagoev B. , Gnad F. , Macek B. , Kumar C. , Mortensen P. , Mann M.
      Cell127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-129 AND THR-178;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    13. 14.
      "Mechanism of polymerase II transcription repression by the histone variant macroH2A."
      Doyen C.-M. , An W. , Angelov D. , Bondarenko V. , Mietton F. , Studitsky V.M. , Hamiche A. , Roeder R.G. , Bouvet P. , Dimitrov S.
      Mol. Cell. Biol.26:1156-1164(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    14. 15.
      "Mapping post-translational modifications of the histone variant MacroH2A1 using tandem mass spectrometry."
      Chu F. , Nusinow D.A. , Chalkley R.J. , Plath K. , Panning B. , Burlingame A.L.
      Mol. Cell. Proteomics5:194-203(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: METHYLATION AT LYS-18 AND LYS-123;PHOSPHORYLATION AT THR-129;IDENTIFICATION BY MASS SPECTROMETRY
    15. 16.
      "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."
      Yu L.R. , Zhu Z. , Chan K.C. , Issaq H.J. , Dimitrov D.S. , Veenstra T.D.
      J. Proteome Res.6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-129;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    16. 17.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-170 AND THR-178;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    17. 18.
      "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
      Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    18. 19.
      "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
      Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-129; SER-170; SER-173 AND THR-178;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Leukemic T-cell.
    19. 20.
      "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
      Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-129 AND THR-178;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    20. 21.
      "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T. , Prokhorova T.A. , Akimov V. , Henningsen J. , Johansen P.T. , Kratchmarova I. , Kassem M. , Mann M. , Olsen J.V. , Blagoev B.
      Sci. Signal.4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-129 AND SER-170;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    21. 22.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 1-120 IN COMPLEX WITH THE NUCLEOSOME CORE PARTICLE;FUNCTION
    22. 23.
      "Splicing regulates NAD metabolite binding to histone macroH2A."
      Kustatscher G. , Hothorn M. , Pugieux C. , Scheffzek K. , Ladurner A.G.
      Nat. Struct. Mol. Biol.12:624-625(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.54 ANGSTROMS) OF 162-372 (ISOFORM 1);X-RAY CRYSTALLOGRAPHY (2.92 ANGSTROMS) OF 161-372 (ISOFORM 2);BINDING TO ADP-RIBOSE AND O-ACETYL-ADP-RIBOSE (ISOFORM 1)
    23. 24.
      "Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases."
      Zhuang M. , Calabrese M.F. , Liu J. , Waddell M.B. , Nourse A. , Hammel M. , Miller D.J. , Walden H. , Duda D.M. , Seyedin S.N. , Hoggard T. , Harper J.W. , White K.P. , Schulman B.A.
      Mol. Cell36:39-50(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.43 ANGSTROMS) OF 172-186 IN COMPLEXES WITH SPOP;SUBUNIT;UBIQUITINATION
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