Cd74 ELISA Kit for Mouse(Mus musculus) | EIAab.comCd74 ELISA Kit for Mouse(Mus musculus)

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Cd74 (Gene name),

H-2 class II histocompatibility antigen gamma chain (Protein name ), HG2A_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Cd74(Ii);

Protein name:

H-2 class II histocompatibility antigen gamma chain;

Alternative:

MHC class II-associated invariant chain;Ia antigen-associated invariant chain(Ii);

CD:

CD74;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Nonamer composed of three alpha/beta/gamma heterotrimers.

Function:

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place.

Subcellular Location:

Membrane Single-pass type II membrane protein

Protein Attributes:

Sequence length:

279

Sequence:

50:

100:

150:

200:

250:

279:

RHNCSEPLDM | EDLSSGLGVT | RQELGQVTL

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

SMR:

P04441

STRING:

10090.ENSMUSP00000095171

Pfam:

PF09307

UniGene:

Mm.439737

KEGG:

mmu:16149

String:

P04441

Uniprot:

P04441

FOR

ELISA Kit for Mouse H-2 class II histocompatibility antigen gamma chain

Cat.:

E13627h

Price:

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Manual:

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Packing:

96T

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ELISA Kit for Mouse H-2 class II histocompatibility antigen gamma chain

Cat.:

E13627r

Price:

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MSDS:

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Packing:

96T

ELISA Kit for Mouse H-2 class II histocompatibility antigen gamma chain

Cat.:

E13627m

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

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CLIA Kit for Mouse H-2 class II histocompatibility antigen gamma chain

MSDS:

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CLIA Kit for Mouse H-2 class II histocompatibility antigen gamma chain

Cat.:

U13627h

Price:

Please sign in first.

Manual:

Looking for manual.

MSDS:

Please sign in first.

Packing:

96T

Range:

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CLIA Kit for Mouse H-2 class II histocompatibility antigen gamma chain

Cat.:

U13627m

Price:

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MSDS:

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Packing:

96T

Polyclonal Antibody for Mouse H-2 class II histocompatibility antigen gamma chain

Monoclonal Antibody for Mouse H-2 class II histocompatibility antigen gamma chain

Protein for Mouse H-2 class II histocompatibility antigen gamma chain

R&D Technical Data

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 5.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 9.3%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	94-98
Plasma	97	93-99

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	89-99%	90-101%	93-101%	92-106%
EDTA plasma(n=5)	89-97%	90-102%	89-102%	90-106%
heparin plasma(n=5)	93-104%	94-108%	101-109%	93-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Primary structure of the gene for the murine Ia antigen-associated invariant chains (Ii). An alternatively spliced exon encodes a cysteine-rich domain highly homologous to a repetitive sequence of thyroglobulin."
Koch N. , Lauer W. , Habicht J. , Dobberstein B.
EMBO J.6:1677-1683(1987) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM LONG);ALTERNATIVE SPLICING
strain: AKR.
2.

"Complete sequence of the murine invariant chain (Ii) gene."
Zhu L. , Jones P.P.
Nucleic Acids Res.17:447-448(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
strain: AKR.
tissue: Liver.
3.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG)
tissue: Mammary gland.
4.

"Nucleotide sequences of the murine Ia-associated invariant chain (Ii) and I-E (H-2S, Beta) chain expressible cDNA clones."
Stone J. , Perry R. , Todd J.A. , McDevitt H.O.
Submitted (1988-03) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-58
tissue: Spleen.
5.

"The IFN-gamma response of the murine invariant chain gene is mediated by a complex enhancer that includes several MHC class II consensus elements."
Eades A.-M. , Litfin M. , Rahmsdorf H.J.
J. Immunol.144:4399-4409(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-25
6.

"Structure of the murine Ia-associated invariant (Ii) chain as deduced from a cDNA clone."
Singer P.A. , Lauer W. , Dembic Z. , Mayer W.E. , Lipp J. , Koch N. , Hammerling G. , Klein J. , Dobberstein B.
EMBO J.3:873-877(1984) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 47-279 (ISOFORM SHORT)
7.

"Identification of the glycosaminoglycan-attachment site of mouse invariant-chain proteoglycan core protein by site-directed mutagenesis."
Miller J. , Hatch J.A. , Simonis S. , Cullen S.E.
Proc. Natl. Acad. Sci. U.S.A.85:1359-1363(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT SER-265;MUTAGENESIS OF SER-265
8.

"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M. , English L. , Lemieux S. , Courcelles M. , Desjardins M. , Thibault P.
Immunity30:143-154(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]

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