Heat shock factor protein 1 (Protein name
), HSF1_BOVIN from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Gene name:
HSF1;
Protein name:
Heat shock factor protein 1(HSF 1);
Alternative:
Heat shock transcription factor 1(HSTF 1);
Organism:
Bovine (Bos taurus).
General Annotation
Sub Unit:
Monomer (By similarity). Under normal conditions, interacts with HSP90AA1 in the HSP90 multichaperone complex; the interaction prevents trimerization and activation of HSF1. On activation by heat-stress or by other factors such as metal ions, HSF1 is released from the complex, homotrimerizes, is hyperphosphorylated and translocated to the nucleus where, subsequently, it can activate transcription. Binds the complex through the regulatory domain. Interacts with SYMPK and CSTF2 in heat-stressed cells. Interacts with FKBP4 in the HSP90 multichaperone complex; the interaction is independent of the phosphorylation state of HSF1. Interacts with MAPKAPK2.
Function:
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Subcellular Location:
Cytoplasm
Nucleus
Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤6.5%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤11.3%
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Recovery
Recovery was determined by spiking various levels of HSF1 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
97
90-99
Plasma
98
96-102
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
93-100%
91-104%
95-99%
92-102%
EDTA plasma(n=5)
90-103%
94-100%
94-102%
91-105%
heparin plasma(n=5)
96-99%
92-102%
90-106%
93-100%
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