HSF1 ELISA Kit for Human(Homo sapiens) | EIAab.comHSF1 ELISA Kit for Human(Homo sapiens)

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HSF1 (Gene name),

Heat shock factor protein 1 (Protein name ), HSF1_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (1)

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Gene name:

HSF1(HSTF1);

Protein name:

Heat shock factor protein 1(HSF 1);

Alternative:

Heat shock transcription factor 1(HSTF 1);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Monomer. Under normal conditions, interacts with HSP90AA1 in the HSP90 multichaperone complex; the interaction prevents trimerization and activation of HSF1. On activation by heat-stress or by other factors such as metal ions, HSF1 is released from the complex, homotrimerizes, is hyperphosphorylated and translocated to the nucleus where, subsequently, it can activate transcription. Binds the complex through the regulatory domain. Interacts with SYMPK and CSTF2 in heat-stressed cells. Interacts with FKBP4 in the HSP90 multichaperone complex; the interaction is independent of the phosphorylation state of HSF1. Interacts with MAPKAPK2.

Function:

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Subcellular Location:

Cytoplasm Nucleus Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.

Protein Attributes:

Sequence length:

529

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

529:

SEGDGFAEDP | TISLLTGSEP | PKAKDPTVS

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

Q00613

STRING:

9606.ENSP00000332698

UniGene:

Hs.530227

KEGG:

hsa:3297

Pfam:

PF00447

SMR:

Q00613

MIM:

140580

Uniprot:

Q00613

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ELISA Kit for Human HSF 1

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E0435h

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96T

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ELISA Kit for Human HSF 1

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E0435m

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96T

ELISA Kit for Human HSF 1

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E0435b

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96T

ELISA Kit for Human HSF 1

Cat.:

E0435c

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96T

CLIA Kit for Human HSF 1

Cat.:

U0435b

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96T

CLIA Kit for Human HSF 1

Cat.:

U0435h

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96T

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CLIA Kit for Human HSF 1

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U0435c

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96T

CLIA Kit for Human HSF 1

Cat.:

U0435m

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96T

Polyclonal Antibody for Human HSF 1

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P0435Rb-m

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40ug/0.2ml

Polyclonal Antibody for Human HSF 1

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P0435Rb-h

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40ug/0.2ml

Monoclonal Antibody for Human HSF 1

Protein for Human HSF 1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.3%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of HSF1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	90-99
Plasma	98	96-102

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-100%	91-104%	95-99%	92-102%
EDTA plasma(n=5)	90-103%	94-100%	94-102%	91-105%
heparin plasma(n=5)	96-99%	92-102%	90-106%	93-100%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"Fractional excretion as a new marker of tubular damage in children with chronic kidney disease"

K Musiał, D Zwolińska.

From:Clinica Chimica Acta

References

1.

"Molecular cloning and expression of a human heat shock factor, HSF1."
Rabindran S.K. , Giorgi G. , Clos J. , Wu C.
Proc. Natl. Acad. Sci. U.S.A.88:6906-6910(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
2.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG)
3.

"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
Submitted (2003-05) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG)
4.

"DNA sequence and analysis of human chromosome 8."
Nusbaum C. , Mikkelsen T.S. , Zody M.C. , Asakawa S. , Taudien S. , Garber M. , Kodira C.D. , Schueler M.G. , Shimizu A. , Whittaker C.A. , Chang J.L. , Cuomo C.A. , Dewar K. , FitzGerald M.G. , Yang X. , Allen N.R. , Anderson S. , Asakawa T. , more...
Nature439:331-335(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
5.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG)
tissue: Muscle.
6.

"Isolation of a cDNA for HSF2: evidence for two heat shock factor genes in humans."
Schuetz T.J. , Gallo G.J. , Sheldon L. , Tempst P. , Kingston R.E.
Proc. Natl. Acad. Sci. U.S.A.88:6911-6915(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 73-79; 81-93; 97-106; 163-170 AND 337-352
7.

"Phosphorylation of serine 230 promotes inducible transcriptional activity of heat shock factor 1."
Holmberg C.I. , Hietakangas V. , Mikhailov A. , Rantanen J.O. , Kallio M. , Meinander A. , Hellman J. , Morrice N. , MacKintosh C. , Morimoto R.I. , Eriksson J.E. , Sistonen L.
EMBO J.20:3800-3810(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 228-241 AND 297-310;PHOSPHORYLATION AT SER-230; SER-303 AND SER-307;FUNCTION;SUBCELLULAR LOCATION;MUTAGENESIS OF SER-230
8.

"A heat shock-responsive domain of human HSF1 that regulates transcription activation domain function."
Green M. , Schuetz T.J. , Sullivan E.K. , Kingston R.E.
Mol. Cell. Biol.15:3354-3362(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DOMAINS
9.

"Repression of human heat shock factor 1 activity at control temperature by phosphorylation."
Knauf U. , Newton E.M. , Kyriakis J. , Kingston R.E.
Genes Dev.10:2782-2793(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-303 AND SER-307;FUNCTION;MUTAGENESIS OF ARG-296; VAL-297; LYS-298; GLU-299; GLU-300; SER-303; SER-307; ARG-309 AND GLU-311
10.

"Repression of the heat shock factor 1 transcriptional activation domain is modulated by constitutive phosphorylation."
Kline M.P. , Morimoto R.I.
Mol. Cell. Biol.17:2107-2115(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-303 AND SER-307;FUNCTION;MUTAGENESIS OF SER-303 AND SER-307
11.

"Transcriptional activation of heat shock factor HSF1 probed by phosphopeptide analysis of factor 32P-labeled in vivo."
Xia W. , Guo Y. , Vilaboa N. , Zuo J. , Voellmy R.
J. Biol. Chem.273:8749-8755(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-307;FUNCTION;MUTAGENESIS OF SER-275; SER-303 AND SER-307
12.

"Regulation of heat shock transcription factor 1 by stress-induced SUMO-1 modification."
Hong Y. , Rogers R. , Matunis M.J. , Mayhew C.N. , Goodson M.L. , Park-Sarge O.K. , Sarge K.D.
J. Biol. Chem.276:40263-40267(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUMOYLATION AT LYS-298;MUTAGENESIS OF LYS-298
13.

"Evidence for a mechanism of repression of heat shock factor 1 transcriptional activity by a multichaperone complex."
Guo Y. , Guettouche T. , Fenna M. , Boellmann F. , Pratt W.B. , Toft D.O. , Smith D.F. , Voellmy R.
J. Biol. Chem.276:45791-45799(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH FKBP4 AND HSP90AA1 IN THE HSP90 MULTICHAPERONE COMPLEX;SUBUNIT;PHOSPHORYLATION;FUNCTION
14.

"Transcriptional activity and DNA binding of heat shock factor-1 involve phosphorylation on threonine 142 by CK2."
Soncin F. , Zhang X. , Chu B. , Wang X. , Asea A. , Ann Stevenson M. , Sacks D.B. , Calderwood S.K.
Biochem. Biophys. Res. Commun.303:700-706(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-142;FUNCTION;MUTAGENESIS OF THR-142
15.

"Phosphorylation of serine 303 is a prerequisite for the stress-inducible SUMO modification of heat shock factor 1."
Hietakangas V. , Ahlskog J.K. , Jakobsson A.M. , Hellesuo M. , Sahlberg N.M. , Holmberg C.I. , Mikhailov A. , Palvimo J.J. , Pirkkala L. , Sistonen L.
Mol. Cell. Biol.23:2953-2968(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUMOYLATION AT LYS-298;PHOSPHORYLATION AT SER-303;FUNCTION;SUBCELLULAR LOCATION;MUTAGENESIS OF LYS-91; LYS-126; LYS-150; LYS-162; SER-230; LYS-298; SER-303; SER-307; SER-363 AND LYS-381
16.

"HSF1 modulation of Hsp70 mRNA polyadenylation via interaction with symplekin."
Xing H. , Mayhew C.N. , Cullen K.E. , Park-Sarge O.-K. , Sarge K.D.
J. Biol. Chem.279:10551-10555(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SYMPK AND CSTF2
17.

"Analysis of phosphorylation of human heat shock factor 1 in cells experiencing a stress."
Guettouche T. , Boellmann F. , Lane W.S. , Voellmy R.
BMC Biochem.6:4-4(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-121; SER-230; SER-292; SER-303; SER-307; SER-314; SER-326; SER-344; SER-363; SER-419 AND SER-444;MUTAGENESIS OF SER-326;IDENTIFICATION BY MASS SPECTROMETRY
18.

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V. , Blagoev B. , Gnad F. , Macek B. , Kumar C. , Mortensen P. , Mann M.
Cell127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-323;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
19.

"Phosphorylation of HSF1 by MAPK-activated protein kinase 2 on serine 121, inhibits transcriptional activity and promotes HSP90 binding."
Wang X. , Khaleque M.A. , Zhao M.J. , Zhong R. , Gaestel M. , Calderwood S.K.
J. Biol. Chem.281:782-791(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-121;FUNCTION;INTERACTION WITH HSP90AA1 AND MAPKAPK2;MUTAGENESIS OF THR-120; SER-121; SER-123; THR-124; THR-527 AND SER-529;IDENTIFICATION BY MASS SPECTROMETRY
20.

"PDSM, a motif for phosphorylation-dependent SUMO modification."
Hietakangas V. , Anckar J. , Blomster H.A. , Fujimoto M. , Palvimo J.J. , Nakai A. , Sistonen L.
Proc. Natl. Acad. Sci. U.S.A.103:45-50(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUMOYLATION AT LYS-298;PHOSPHORYLATION AT SER-303
21.

"Nine-amino-acid transactivation domain: establishment and prediction utilities."
Piskacek S. , Gregor M. , Nemethova M. , Grabner M. , Kovarik P. , Piskacek M.
Genomics89:756-768(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DOMAIN
22.

"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T. , Yi W. , Lu B. , Park S.K. , Xu T. , Lee J.-D. , Yates J.R. III
J. Proteome Res.7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-314;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
23.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-363;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
24.

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
25.

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-314; THR-323 AND SER-326;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Leukemic T-cell.
26.

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-314 AND SER-326;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
27.

"Transformation of eEF1Bdelta into heat-shock response transcription factor by alternative splicing."
Kaitsuka T. , Tomizawa K. , Matsushita M.
EMBO Rep.12:673-681(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH EEF1D
28.

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T. , Prokhorova T.A. , Akimov V. , Henningsen J. , Johansen P.T. , Kratchmarova I. , Kassem M. , Mann M. , Olsen J.V. , Blagoev B.
Sci. Signal.4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]

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