HUWE1 ELISA Kit for Human(Homo sapiens) | EIAab.comHUWE1 ELISA Kit for Human(Homo sapiens)

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HUWE1 (Gene name),

E3 ubiquitin-protein ligase HUWE1 (Protein name ), HUWE1_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

HUWE1(HSPC272;KIAA0312;KIAA1578;UREB1);

Protein name:

E3 ubiquitin-protein ligase HUWE1;

Alternative:

HECT, UBA and WWE domain-containing protein 1;ARF-binding protein 1(ARF-BP1);Large structure of UREB1(LASU1);Homologous to E6AP carboxyl terminus homologous protein 9(HectH9);Upstream regulatory element-binding protein 1(URE-B1;URE-binding protein 1);Mcl-1 ubiquitin ligase E3(Mule);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Interacts with isoform p14ARF of CDKN2A which strongly inhibits HUWE1 ubiquitin ligase activity. Interacts with MYCN, POLB and CDC6.

Function:

E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation.

Subcellular Location:

Cytoplasm Nucleus Mainly expressed in the cytoplasm of most tissues, except in the nucleus of spermatogonia, primary spermatocytes and neuronal cells (By similarity). Predominantly cytosolic or perinuclear in some colorectal carcinoma cells.

Protein Attributes:

Sequence length:

4374

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ESFEKLRHML | LLAIQECSEG | FGLA

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

MIM:

300697

String:

Q7Z6Z7

KEGG:

hsa:10075

Pfam:

PF14377

SMR:

Q7Z6Z7

UniGene:

Hs.136905

Pfam:

PF06012

Uniprot:

Q7Z6Z7

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ELISA Kit for Human E3 ubiquitin-protein ligase HUWE1

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ELISA Kit for Human E3 ubiquitin-protein ligase HUWE1

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E2369m

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96T

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ELISA Kit for Human E3 ubiquitin-protein ligase HUWE1

Cat.:

E2369h

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96T

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CLIA Kit for Human E3 ubiquitin-protein ligase HUWE1

Cat.:

U2369h

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Looking for manual.

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Packing:

96T

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CLIA Kit for Human E3 ubiquitin-protein ligase HUWE1

Cat.:

U2369m

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MSDS:

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Packing:

96T

CLIA Kit for Human E3 ubiquitin-protein ligase HUWE1

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Polyclonal Antibody for Human E3 ubiquitin-protein ligase HUWE1

Cat.:

P2369Rb-m

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40ug/0.2ml

Polyclonal Antibody for Human E3 ubiquitin-protein ligase HUWE1

Cat.:

P2369Rb-h

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Monoclonal Antibody for Human E3 ubiquitin-protein ligase HUWE1

Protein for Human E3 ubiquitin-protein ligase HUWE1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor."
Chen D. , Kon N. , Li M. , Zhang W. , Qin J. , Gu W.
Cell121:1071-1083(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);FUNCTION;INTERACTION WITH CDKN2A;MUTAGENESIS OF CYS-4341
2.

"Mule/ARF-BP1, a BH3-only E3 ubiquitin ligase, catalyzes the polyubiquitination of Mcl-1 and regulates apoptosis."
Zhong Q. , Gao W. , Du F. , Wang X.
Cell121:1085-1095(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);FUNCTION;MUTAGENESIS OF CYS-4341
3.

"Characterization of E3Histone, a novel testis ubiquitin protein ligase which ubiquitinates histones."
Liu Z. , Oughtred R. , Wing S.S.
Mol. Cell. Biol.25:2819-2831(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);FUNCTION
4.

"The DNA sequence of the human X chromosome."
Ross M.T. , Grafham D.V. , Coffey A.J. , Scherer S. , McLay K. , Muzny D. , Platzer M. , Howell G.R. , Burrows C. , Bird C.P. , Frankish A. , Lovell F.L. , Howe K.L. , Ashurst J.L. , Fulton R.S. , Sudbrak R. , Wen G. , Jones M.C. , more...
Nature434:325-337(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
5.

"Homo sapiens LASU1 (large structure of UREB1) mRNA, complete cds."
Miyazaki K. , Okamoto Y. , Sakamoto M. , Kato C. , Ozaki T. , Watanabe K. , Nakagawara A.
Submitted (2001-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 959-4374 (ISOFORM 3)
tissue: Brain.
6.

"Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
Nagase T. , Ishikawa K. , Nakajima D. , Ohira M. , Seki N. , Miyajima N. , Tanaka A. , Kotani H. , Nomura N. , Ohara O.
DNA Res.4:141-150(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1166-4374 (ISOFORM 2)
tissue: Brain.
7.

Ohara O. , Nagase T. , Kikuno R. , Nomura N.
Submitted (2011-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SEQUENCE REVISION TO 2310
8.

"Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T. , Kikuno R. , Nakayama M. , Hirosawa M. , Ohara O.
DNA Res.7:273-281(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1246-2418
tissue: Brain.
9.

"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Zhang Q.-H. , Ye M. , Wu X.-Y. , Ren S.-X. , Zhao M. , Zhao C.-J. , Fu G. , Shen Y. , Fan H.-Y. , Lu G. , Zhong M. , Xu X.-R. , Han Z.-G. , Zhang J.-W. , Tao J. , Huang Q.-H. , Zhou J. , Hu G.-X. , more...
Genome Res.10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2451-4374
tissue: Umbilical cord blood.
10.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3953-4374
tissue: Ovary.
tissue: Placenta.
11.

Kim J.W. , Lee Y. , Hong Y.M. , Hong M. , Choe I.S.
Submitted (1998-04) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4029-4374
12.

"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L. , Schick M. , Neubert P. , Schatten R. , Henze S. , Korn B.
Submitted (2004-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4067-4374
13.

"The full-ORF clone resource of the German cDNA consortium."
Bechtel S. , Rosenfelder H. , Duda A. , Schmidt C.P. , Ernst U. , Wellenreuther R. , Mehrle A. , Schuster C. , Bahr A. , Bloecker H. , Heubner D. , Hoerlein A. , Michel G. , Wedler H. , Koehrer K. , Ottenwaelder B. , Poustka A. , Wiemann S. , more...
BMC Genomics8:399-399(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4320-4374
tissue: Melanoma.
14.

"Over-expression of human UREB1 in colorectal cancer: HECT domain of human UREB1 inhibits the activity of tumor suppressor p53 protein."
Yoon S.Y. , Lee Y. , Kim J.H. , Chung A.-S. , Joo J.H. , Kim C.-N. , Kim N.-S. , Choe I.S. , Kim J.W.
Biochem. Biophys. Res. Commun.326:7-17(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;TISSUE SPECIFICITY;MUTAGENESIS OF TYR-4268 AND CYS-4341
15.

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V. , Blagoev B. , Gnad F. , Macek B. , Kumar C. , Mortensen P. , Mann M.
Cell127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2362;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
16.

"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A. , Villen J. , Gerber S.A. , Rush J. , Gygi S.P.
Nat. Biotechnol.24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1395; SER-1907; SER-2362; SER-2887 AND SER-2918;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
17.

"Cdc6 stability is regulated by the Huwe1 ubiquitin ligase after DNA damage."
Hall J.R. , Kow E. , Nevis K.R. , Lu C.K. , Luce K.S. , Zhong Q. , Cook J.G.
Mol. Biol. Cell18:3340-3350(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH CDC6
18.

"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S. , Ballif B.A. , Smogorzewska A. , McDonald E.R. III , Hurov K.E. , Luo J. , Bakalarski C.E. , Zhao Z. , Solimini N. , Lerenthal Y. , Shiloh Y. , Gygi S.P. , Elledge S.J.
Science316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Embryonic kidney.
19.

"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T. , Yi W. , Lu B. , Park S.K. , Xu T. , Lee J.-D. , Yates J.R. III
J. Proteome Res.7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1084;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
20.

"Phosphoproteome of resting human platelets."
Zahedi R.P. , Lewandrowski U. , Wiesner J. , Wortelkamp S. , Moebius J. , Schuetz C. , Walter U. , Gambaryan S. , Sickmann A.
J. Proteome Res.7:526-534(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Platelet.
21.

"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H. , Olsen J.V. , Bairlein M. , Gnad F. , Oppermann F.S. , Korner R. , Greff Z. , Keri G. , Stemmann O. , Mann M.
Mol. Cell31:438-448(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2362;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
22.

"The HECT-domain ubiquitin ligase Huwe1 controls neural differentiation and proliferation by destabilizing the N-Myc oncoprotein."
Zhao X. , Heng J.I.-T. , Guardavaccaro D. , Jiang R. , Pagano M. , Guillemot F. , Iavarone A. , Lasorella A.
Nat. Cell Biol.10:643-653(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH MYCN;MUTAGENESIS OF CYS-4341
23.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1368; SER-1907; SER-2362; SER-2365; SER-2887; SER-3662; SER-3919; THR-3924 AND THR-3927;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
24.

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
25.

"Ubiquitin ligase ARF-BP1/Mule modulates base excision repair."
Parsons J.L. , Tait P.S. , Finch D. , Dianova I.I. , Edelmann M.J. , Khoronenkova S.V. , Kessler B.M. , Sharma R.A. , McKenna W.G. , Dianov G.L.
EMBO J.28:3207-3215(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;INTERACTION WITH POLB
26.

"Human BRE1 is an E3 ubiquitin ligase for Ebp1 tumor suppressor."
Liu Z. , Oh S.M. , Okada M. , Liu X. , Cheng D. , Peng J. , Brat D.J. , Sun S.Y. , Zhou W. , Gu W. , Ye K.
Mol. Biol. Cell20:757-768(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH PA2G4
27.

"Large-scale proteomics analysis of the human kinome."
Oppermann F.S. , Gnad F. , Olsen J.V. , Hornberger R. , Greff Z. , Keri G. , Mann M. , Daub H.
Mol. Cell. Proteomics8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
28.

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1370; SER-1395; SER-1907; SER-2362; SER-2391; SER-2595; SER-2619; THR-2751; SER-2887; SER-3116; SER-3752; SER-3757; SER-3808; SER-3816; SER-3919 AND THR-3924;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Leukemic T-cell.
29.

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1084; SER-1368; SER-1395; THR-1722; SER-1907; SER-2362; SER-2365; SER-2595; SER-3127; SER-3662; SER-3757; SER-3760 AND SER-3919;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
30.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
31.

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T. , Prokhorova T.A. , Akimov V. , Henningsen J. , Johansen P.T. , Kratchmarova I. , Kassem M. , Mann M. , Olsen J.V. , Blagoev B.
Sci. Signal.4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1084; SER-1907; SER-2362 AND SER-2365;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
32.

"Solution structure of RSGI RUH-074, a human UBA domain."
RIKEN structural genomics initiative (RSGI)
Submitted (2009-02) to the PDB data bank

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 1317-1356
33.

"Hect domain of human huwe1/mule."
Structural genomics consortium (SGC)
Submitted (2009-05) to the PDB data bank

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 4005-4374
34.

"Submicroscopic duplications of the hydroxysteroid dehydrogenase HSD17B10 and the E3 ubiquitin ligase HUWE1 are associated with mental retardation."
Froyen G. , Corbett M. , Vandewalle J. , Jarvela I. , Lawrence O. , Meldrum C. , Bauters M. , Govaerts K. , Vandeleur L. , Van Esch H. , Chelly J. , Sanlaville D. , van Bokhoven H. , Ropers H.-H. , Laumonnier F. , Ranieri E. , Schwartz C.E. , Abidi F. , more...
Am. J. Hum. Genet.82:432-443(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS MRXS-TURNER HIS-2981; TRP-4013 AND CYS-4187;INVOLVEMENT IN MRX17

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