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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.7%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.1%
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Recovery
Recovery was determined by spiking various levels of IL-18 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
102
96-106
Plasma
101
99-104
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-18 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
100-105%
94-101%
95-102%
92-97%
EDTA plasma(n=5)
90-95%
94-106%
105-107%
87-98%
heparin plasma(n=5)
95-103%
91-99%
104-107%
98-105%
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Cited for: NUCLEOTIDE SEQUENCE [MRNA];PARTIAL PROTEIN SEQUENCE