Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
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s
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤6.7%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤10.2%
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Recovery
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Recovery was determined by spiking various levels of IL1B into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
102
96-104
Plasma
101
99-106
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL1B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
96-108%
94-102%
96-107%
96-108%
Saliva(n=3)
100-105%
95-108%
95-108%
97-106%
heparin plasma(n=5)
95-104%
96-107%
99-105%
98-104%
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"Molecular cloning and expression of caprine IL-1alpha and IL-1beta." Takakura H.
,
Hashimoto O.
,
Mori Y.
,
Tatsumi M.
Submitted (1995-06) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [MRNA]
Vaild Sequence:
