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Index > Protein center > IL1B(Gene name) > Human
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  • IL1B (Gene name),
  • Interleukin-1 beta (Protein name ),  IL1B_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Gene name:
    IL1B(IL1F2);
    Protein name:
    Interleukin-1 beta(IL-1 beta);
    Alternative:
    Catabolin;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Monomer.
    Function:
    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
    Subcellular Location:
    Secreted The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
    Protein Attributes:
    Sequence length:
    269
    Sequence:
    50:
    MAEVPELASE | MMAYYSGNED | DLFFEADGPK | QMKCSFQDLD | LCPLDGGIQL | 
    100:
    RISDHHYSKG | FRQAASVVVA | MDKLRKMLVP | CPQTFQENDL | STFFPFIFEE | 
    150:
    EPIFFDTWDN | EAYVHDAPVR | SLNCTLRDSQ | QKSLVMSGPY | ELKALHLQGQ | 
    200:
    DMEQQVVFSM | SFVQGEESND | KIPVALGLKE | KNLYLSCVLK | DDKPTLQLES | 
    250:
    VDPKNYPKKK | MEKRFVFNKI | EINNKLEFES | AQFPNWYIST | SQAENMPVFL | 
    269:
    GGTKGGQDIT | DFTMQFVSS
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    String:
    KEGG:
    MIM:
    UniGene:
    SMR:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Human IL-1 beta
    Cat.:
    E0563b
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human IL-1 beta
    Cat.:
    E0563h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human IL-1 beta
    Cat.:
    E0563d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human IL-1 beta
    MSDS:
    Please sign in first.
    ELISA Kit for Human IL-1 beta
    Cat.:
    E0563p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human IL-1 beta
    Cat.:
    E0563r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human IL-1 beta
    Cat.:
    E0563m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human IL-1 beta
    Cat.:
    E0563g
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human IL-1 beta
    Cat.:
    U0563m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human IL-1 beta
    Cat.:
    U0563b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human IL-1 beta
    Cat.:
    U0563r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human IL-1 beta
    MSDS:
    Please sign in first.
    CLIA Kit for Human IL-1 beta
    Cat.:
    U0563p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human IL-1 beta
    Cat.:
    U0563d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human IL-1 beta
    Cat.:
    U0563h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human IL-1 beta
    Polyclonal Antibody for Human IL-1 beta
    Cat.:
    P0563Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human IL-1 beta
    Cat.:
    P0563Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human IL-1 beta
    Polyclonal Antibody for Human IL-1 beta
    Polyclonal Antibody for Human IL-1 beta
    Cat.:
    P0563Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human IL-1 beta
    Monoclonal Antibody for Human IL-1 beta
    Monoclonal Antibody for Human IL-1 beta
    Monoclonal Antibody for Human IL-1 beta
    Monoclonal Antibody for Human IL-1 beta
    Monoclonal Antibody for Human IL-1 beta
    Monoclonal Antibody for Human IL-1 beta
    Monoclonal Antibody for Human IL-1 beta
    Protein for Human IL-1 beta
    Protein for Human IL-1 beta
    Protein for Human IL-1 beta
    Protein for Human IL-1 beta
    Protein for Human IL-1 beta
    Protein for Human IL-1 beta
    Protein for Human IL-1 beta
    Cat.:
    R0563h
    Price:
    Please sign in first.
    Packing:
    1mg
    Purity:
    >90%

    R&D Technical Data
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    s

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤6.7%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤10.2%

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    Recovery
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    Recovery was determined by spiking various levels of IL1B into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    102

    96-104

    Plasma

    101

    99-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL1B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    96-108%

    94-102%

    96-107%

    96-108%

    Saliva(n=3)

    100-105%

    95-108%

    95-108%

    97-106%

    heparin plasma(n=5)

    95-104%

     

    96-107%

    99-105%

    98-104%

     

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    Citations
    [1].
    Jing Tang, Xi Chen, Weifeng Tu, Yuanbo Guo, Zhenlong Zhao, Qiong Xue, Chunshui Lin, Jinfang Xiao, Xuegang Sun, Tao Tao, Miaoning Gu, Youtan Liu
    References
    1. 1.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    2. 2.
      "Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs."
      March C.J. , Mosley B. , Larsen A. , Cerretti D.P. , Braedt G. , Price V. , Gillis S. , Henney C.S. , Kronheim S.R. , Grabstein K. , Conlon P.J. , Hopp T.P. , Cosman D.
      Nature315:641-647(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    3. 3.
      "Genomic sequence for human prointerleukin 1 beta: possible evolution from a reverse transcribed prointerleukin 1 alpha gene."
      Clark B.D. , Collins K.L. , Gandy M.S. , Webb A.C. , Auron P.E.
      Nucleic Acids Res.14:7897-7914(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
      tissue: Leukocyte.
    4. 4.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM
    5. 5.
      "cDNA cloning of IL-1 alpha and IL-1 beta from mRNA of U937 cell line."
      Nishida T. , Nishino N. , Takano M. , Kawai K. , Bando K. , Masui Y. , Nakai S. , Hirai Y.
      Biochem. Biophys. Res. Commun.143:345-352(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Histiocytic lymphoma.
    6. 6.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    7. 7.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Monocyte.
    8. 8.
      "A sequence-based map of the nine genes of the human interleukin-1 cluster."
      Nicklin M.J.H. , Barton J.L. , Nguyen M. , Fitzgerald M.G. , Duff W.G. , Kornman K.
      Genomics79:718-725(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    9. 9.
      "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
      Submitted (2003-05) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
    10. 10.
      "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L. , Schick M. , Neubert P. , Schatten R. , Henze S. , Korn B.
      Submitted (2004-05) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
    11. 11.
      SeattleSNPs variation discovery resource
      Submitted (2002-07) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    12. 12.
      "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W. , Graves T.A. , Fulton R.S. , Fulton L.A. , Pepin K.H. , Minx P. , Wagner-McPherson C. , Layman D. , Wylie K. , Sekhon M. , Becker M.C. , Fewell G.A. , Delehaunty K.D. , Miner T.L. , Nash W.E. , Kremitzki C. , Oddy L. , Du H. , more...
      Nature434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    13. 13.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    14. 14.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
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