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s
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s
12% SDS-PAGE Analysis
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s
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.8%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤5.6%
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.2%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.5%
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.4%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤6.4%
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.2%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.5%
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Recovery
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Recovery was determined by spiking various levels of IL6 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
96
91-102
Plasma
98
94-99
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Recovery was determined by spiking various levels of IL-6 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
91
87-94
Plasma
92
89-93
Recovery was determined by spiking various levels of IL-6 into serum and plasma .
Sample Type
Average(%)
Recovery Range (%)
Serum
93
86-99
Plasma
95
86-101
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Recovery was determined by spiking various levels of IL-6 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
90
85-96
Plasma
91
87-93
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
92-98%
90-99%
89-97%
96-102%
EDTA plasma(n=5)
87-95%
92-98%
100-105%
97-106%
heparin plasma(n=5)
91-100%
94-103%
105-108%
91-97%
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
92-96%
90-93%
85-89%
82-87%
EDTA plasma(n=5)
89-95%
92-96%
85-87%
90-95%
heparin plasma(n=5)
86-93%
85-92%
83-87%
91-96%
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
92-101%
93-102%
97-108%
94-110%
EDTA plasma(n=5)
101-105%
97-109%
88-97%
90-105%
heparin plasma(n=5)
96-107%
98-110%
100-111%
90-110%
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
95-93%
92-90%
86-91%
89-94%
EDTA plasma(n=5)
82-86%
86-92%
87-93%
90-96%
heparin plasma(n=5)
91-94%
82-86%
81-93%
84-95%
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