Il6 ELISA Kit for Mouse(Mus musculus) | EIAab.comIl6 ELISA Kit for Mouse(Mus musculus)

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Il6 (Gene name),

Interleukin-6 (Protein name ), IL6_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (5)

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Gene name:

Il6(Il-6);

Protein name:

Interleukin-6(IL-6);

Alternative:

Interleukin HP-1;B-cell hybridoma growth factor;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

N/A

Function:

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

211

Sequence:

50:

100:

150:

200:

211:

LKVTLRSTRQ | T

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

mmu:16193

String:

P08505

UniGene:

Mm.1019

Pfam:

PF00489

SMR:

P08505

Uniprot:

P08505

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ELISA Kit for Mouse IL-6

Cat.:

E0079p

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96T

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ELISA Kit for Mouse IL-6

Cat.:

E0079d

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96T

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ELISA Kit for Mouse IL-6

Cat.:

E0079r

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96T

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ELISA Kit for Mouse IL-6

Cat.:

E0079b

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96T

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ELISA Kit for Mouse IL-6

Cat.:

E0079c

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96T

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ELISA Kit for Mouse IL-6

Cat.:

E0079h

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96T

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ELISA Kit for Mouse IL-6

Cat.:

E0079m

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96T

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ELISA Kit for Mouse IL-6

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CLIA Kit for Mouse IL-6

MSDS:

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CLIA Kit for Mouse IL-6

Cat.:

U0079r

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96T

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CLIA Kit for Mouse IL-6

Cat.:

U0079m

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96T

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CLIA Kit for Mouse IL-6

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U0079p

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96T

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CLIA Kit for Mouse IL-6

Cat.:

U0079h

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Looking for manual.

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Packing:

96T

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CLIA Kit for Mouse IL-6

Cat.:

U0079b

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Looking for manual.

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96T

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CLIA Kit for Mouse IL-6

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U0079d

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Looking for manual.

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96T

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CLIA Kit for Mouse IL-6

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U0079c

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96T

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Polyclonal Antibody for Mouse IL-6

Cat.:

P0079Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse IL-6

Cat.:

P0079Rb-r

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40ug/0.2ml

Polyclonal Antibody for Mouse IL-6

Cat.:

P0079Rb-m

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Packing:

40ug/0.2ml

Monoclonal Antibody for Mouse IL-6

Protein for Mouse IL-6

Cat.:

R0079h

Price:

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Packing:

1mg

Purity:

>90%

Protein for Mouse IL-6

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤5.6%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.4%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of IL6 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	91-102
Plasma	98	94-99

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of IL-6 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	90	85-96
Plasma	91	87-93

Recovery was determined by spiking various levels of IL-6 into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	93	86-99
Plasma	95	86-101

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of IL-6 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	91	87-94
Plasma	92	89-93

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-98%	90-99%	89-97%	96-102%
EDTA plasma(n=5)	87-95%	92-98%	100-105%	97-106%
heparin plasma(n=5)	91-100%	94-103%	105-108%	91-97%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-93%	92-90%	86-91%	89-94%
EDTA plasma(n=5)	82-86%	86-92%	87-93%	90-96%
heparin plasma(n=5)	91-94%	82-86%	81-93%	84-95%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-101%	93-102%	97-108%	94-110%
EDTA plasma(n=5)	101-105%	97-109%	88-97%	90-105%
heparin plasma(n=5)	96-107%	98-110%	100-111%	90-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-96%	90-93%	85-89%	82-87%
EDTA plasma(n=5)	89-95%	92-96%	85-87%	90-95%
heparin plasma(n=5)	86-93%	85-92%	83-87%	91-96%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"Successful Treatment of Acute Lung Injury with Pitavastatin in Septic Mice: Potential Role of Glucocorticoid Receptor Expression in Alveolar Macrophag"

K Takano, S Yamamoto, K Tomita, et al.

From:THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS

[2].

"Staphylococcus aureus supernatant induces the release of mouse β-defensin-14 from osteoblasts via the p38 MAPK and NF-κB pathways"

Chen Zhu, Hui Qin, Tao Cheng, Hong-Lue Tan, Yong-Yuan Guo, Si-Feng Shi, De-Sheng Chen, Xian-Long Zhang

From:Int J Mol Med

[3].

"Neonatal Bacillus Calmette-Guérin vaccination alleviates lipopolysaccharide-induced neurobehavioral impairments and neuroinflammation in adult mice"

Yang J, Qi F, Yao Z.

From:Molecular Medicine Reports

[4].

"In vitro morphology, viability and cytokine secretion of uterine telocyte-activated mouse peritoneal macrophages"

Chi C, Jiang X J, Su L, et al.

From:Journal of cellular and molecular medicine

[5].

"Tetramethylpyrazine (TMP) protects rats against acute pancreatitis through NF-κB pathway"

Ly Chen, Yj Chen, H Yun, et al.

From:Bioengineered

References

1.

"cDNA cloning of murine interleukin-HP1: homology with human interleukin 6."
van Snick J. , Cayphas S. , Szikora J.-P. , Renauld J.-C. , van Roost E. , Boon T. , Simpson R.J.
Eur. J. Immunol.18:193-197(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PARTIAL PROTEIN SEQUENCE
2.

"Genomic structure of the murine IL-6 gene. High degree conservation of potential regulatory sequences between mouse and human."
Tanabe O. , Akira S. , Kamiya T. , Wong G.G. , Hirano T. , Kishimoto T.
J. Immunol.141:3875-3881(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
3.

"Multiple biological activities are expressed by a mouse interleukin 6 cDNA clone isolated from bone marrow stromal cells."
Chiu C.P. , Moulds C. , Coffman R.L. , Rennick D. , Lee F.
Proc. Natl. Acad. Sci. U.S.A.85:7099-7103(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
4.

"Cloning and sequence analysis of the cDNA for murine interleukin-6."
Grenett H.E. , Fuentes N.L. , Fuller G.M.
Nucleic Acids Res.18:6455-6455(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: BALB/c.
5.

"The transcriptional landscape of the mammalian genome."
Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain: C57BL/6J.
tissue: Macrophage.
6.

"DNA rearrangement and constitutive expression of the interleukin 6 gene in a mouse plasmacytoma."
Blankenstein T. , Qin Z. , Li W. , Diamantstein T.
J. Exp. Med.171:965-970(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6
strain: BALB/c.
7.

"The murine Il-6 gene maps to the proximal region of chromosome 5."
Mock B.A. , Nordan R.P. , Justice M.J. , Kozak C. , Jenkins N.A. , Copeland N.G. , Clark S.C. , Wong G.G. , Rudikoff S.
J. Immunol.142:1372-1376(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 5-211
strain: C57BL/6J.
8.

"Purification and NH2-terminal amino acid sequence of a T-cell-derived lymphokine with growth factor activity for B-cell hybridomas."
van Snick J. , Cayphas S. , Vink A. , Uyttenhove C. , Coulie P.G. , Rubira M.R. , Simpson R.J.
Proc. Natl. Acad. Sci. U.S.A.83:9679-9683(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 25-45
9.

"Murine hybridoma/plasmacytoma growth factor. Complete amino-acid sequence and relation to human interleukin-6."
Simpson R.J. , Moritz R.L. , Rubira M.R. , van Snick J.
Eur. J. Biochem.176:187-197(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 25-211
10.

"Internal amino acid sequencing of proteins by in situ cyanogen bromide cleavage in polyacrylamide gels."
Jahnen W. , Ward L.D. , Reid G.E. , Moritz R.L. , Simpson R.J.
Biochem. Biophys. Res. Commun.166:139-145(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 66-75; 78-84 AND 128-148
11.

"Characterization of a recombinant murine interleukin-6: assignment of disulphide bonds."
Simpson R.J. , Moritz R.L. , van Roost E. , van Snick J.
Biochem. Biophys. Res. Commun.157:364-372(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISULFIDE BONDS
12.

"The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells."
Ivanov I.I. , McKenzie B.S. , Zhou L. , Tadokoro C.E. , Lepelley A. , Lafaille J.J. , Cua D.J. , Littman D.R.
Cell126:1121-1133(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN TH17 DIFFERENTIATION;DISRUPTION PHENOTYPE

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