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Index > Protein center > Itih3(Gene name) > Mouse
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  • Itih3 (Gene name),
  • Inter-alpha-trypsin inhibitor heavy chain H3 (Protein name ),  ITIH3_MOUSE from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    Itih3;
    Protein name:
    Inter-alpha-trypsin inhibitor heavy chain H3(ITI heavy chain H3;ITI-HC3;Inter-alpha-inhibitor heavy chain 3);
    Alternative:

    Organism:
    Mouse (Mus musculus). 
    General Annotation
    Sub Unit:
    I-alpha-I plasma protease inhibitors are assembled from one or two heavy chains (H1, H2 or H3) and one light chain, bikunin. Inter-alpha-inhibitor (I-alpha-I) is composed of H1, H2 and bikunin, inter-alpha-like inhibitor (I-alpha-LI) of H2 and bikunin, and pre-alpha-inhibitor (P-alpha-I) of H3 and bikunin.
    Function:
    May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    889
    Sequence:
    50:
    MRTMWWPCLV | LALLSGLETS | GFPRSPLQLL | GKRSLPEGVV | DGIEVYSTKI | 
    100:
    SCKVTSRFAH | NVVTTRAVNR | ADTAKEVSFD | VELPKTAFIT | NFTLTIDGVT | 
    150:
    YPGNVKEKEV | AQKQYEKAVS | QGKTAGLVKA | SGRKLEKFTV | SVNVAAGSKV | 
    200:
    TFELTYEELL | KRNKGKYEMY | LKVQPKQLVR | HFEIDAHIFE | PQGISMLDAE | 
    250:
    ASFITNDLLG | SALTKSFSGK | KGHVSFKPSL | DQQRSCPTCT | DSLLNGDFTI | 
    300:
    VYDVNRESPG | NVQIVNGYFV | HFFAPQGLPV | VPKNIVFVID | VSGSMSGRKI | 
    350:
    QQTREALLKI | LDDVKEDDYL | NFILFSTDVT | TWKDHLVQAT | PANLKEAKTF | 
    400:
    VKNIHDQSMT | NINDGLLKGI | EMLNKAREDH | TVPERSTSII | IMLTDGDANT | 
    450:
    GESRPEKIQE | NVRNAIGGKF | PLYNLGFGNN | LNYNFLETLA | LENHGLARRI | 
    500:
    YEDSDANLQL | QGFYEEVANP | LLTNVEVEYP | ENAILDLTRN | SYPHFYDGSE | 
    550:
    IVVAGRLVDR | NMDNFKADVK | GHGALNDLTF | TEEVDMEEMD | AALKEQGYIF | 
    600:
    GDYIERLWAY | LTIEQLLEKR | KNAKGDEKEN | ITAEALDLSL | KYHFVTPLTS | 
    650:
    MVVTKPEDNE | DQTSIADKPG | EEAIAETTTM | SFLTTQQSSQ | SPYYYVDGDP | 
    700:
    HFIIQIPGKN | DSICFNIDEK | PGTVLRLIQD | PVTGITVTGQ | IIGDKRSNAS | 
    750:
    SRTGKTYFGK | LGITNAWMDF | RVEVTTEKII | LGTGAELSTF | SWLDTITVTQ | 
    800:
    TGLSVTINRK | KNMVVSFGDG | ISFVIILHQV | WKKHPVHQDF | LGFYVVDSHR | 
    850:
    MSAQTHGLLG | QFFQPFDFKV | FGIRPGSDPT | KPDATMVVKN | HRLTVTRGSQ | 
    889:
    KDYRKDASVG | TKVICWFVHN | NGEGLIDGVH | TDYIVPSLF
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
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    Related Databases
    KEGG:
    UniGene:
    Pfam:
    String:
    SMR:
    Uniprot:
     
    FOR
    ELISA Kit for Mouse ITI heavy chain H3
    Cat.:
    E13559r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Mouse ITI heavy chain H3
    Cat.:
    E13559h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Mouse ITI heavy chain H3
    MSDS:
    Please sign in first.
    ELISA Kit for Mouse ITI heavy chain H3
    Cat.:
    E13559b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Mouse ITI heavy chain H3
    Cat.:
    E13559m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Mouse ITI heavy chain H3
    Cat.:
    U13559m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Mouse ITI heavy chain H3
    MSDS:
    Please sign in first.
    CLIA Kit for Mouse ITI heavy chain H3
    Cat.:
    U13559r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse ITI heavy chain H3
    Cat.:
    U13559b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse ITI heavy chain H3
    Cat.:
    U13559h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Mouse ITI heavy chain H3
    Cat.:
    P13559Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse ITI heavy chain H3
    Polyclonal Antibody for Mouse ITI heavy chain H3
    Cat.:
    P13559Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse ITI heavy chain H3
    Polyclonal Antibody for Mouse ITI heavy chain H3
    Cat.:
    P13559Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Mouse ITI heavy chain H3
    Monoclonal Antibody for Mouse ITI heavy chain H3
    Monoclonal Antibody for Mouse ITI heavy chain H3
    Monoclonal Antibody for Mouse ITI heavy chain H3
    Monoclonal Antibody for Mouse ITI heavy chain H3
    Protein for Mouse ITI heavy chain H3
    Protein for Mouse ITI heavy chain H3
    Protein for Mouse ITI heavy chain H3
    Protein for Mouse ITI heavy chain H3
    Protein for Mouse ITI heavy chain H3

    R&D Technical Data
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    s

    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤5.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.8%

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of ITIH3 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    104

    94-105

    Plasma

    102

    93-106

     

     

     

     

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ITIH3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    95-113%

    97-109%

    95-111%

    98-109%

    Saliva(n=3)

    94-108%

    96-108%

    97-107%

    95-107%

    heparin plasma(n=5)

    93-110%

     

    98-105%

    99-106%

    94-110%

     

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    Saliva(n=3)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    References
    1. 1.
      "The transcriptional landscape of the mammalian genome."
      Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
      Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
    2. 2.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
      strain: C57BL/6J.
    3. 3.
      "The three heavy-chain precursors for the inter-alpha-inhibitor family in mouse: new members of the multicopper oxidase protein group with differential transcription in liver and brain."
      Chan P. , Risler J.-L. , Raguenez G. , Salier J.-P.
      Biochem. J.306:505-512(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-889
      strain: C57BL/6N.
      tissue: Liver.
    4. 4.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-889
      strain: FVB/N.
      tissue: Liver.
    5. 5.
      "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
      Ghesquiere B. , Van Damme J. , Martens L. , Vandekerckhove J. , Gevaert K.
      J. Proteome Res.5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-580
      strain: C57BL/6.
      tissue: Plasma.
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