Pkm2 ELISA Kit for Rat(Rattus norvegicus) | EIAab.comPkm2 ELISA Kit for Rat(Rattus norvegicus)

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Pkm2 (Gene name),

Pyruvate kinase isozymes M1/M2 (Protein name ), KPYM_RAT from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Pkm2(Pykm;Pykm);

Protein name:

Pyruvate kinase isozymes M1/M2;

Alternative:

Pyruvate kinase muscle isozyme;

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

Monomer and homotetramer. Exists as a monomer in the absence of FBP, and reversibly associates to form a homotetramer in the presence of FBP. The monomeric form binds T3. Tetramer formation induces pyruvate kinase activity. Interacts with HERC1 POU5F1 and PML.

Function:

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation.

Subcellular Location:

Cytoplasm Nucleus

Protein Attributes:

Sequence length:

531

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

531:

GFFKKGDVVI | VLTGWRPGSG | FTNTMRVVPV | P

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF00224

SMR:

P11980

KEGG:

rno:25630

UniGene:

Rn.1556

String:

P11980

Uniprot:

P11980

FOR

ELISA Kit for Rat Pyruvate kinase isozymes M1/M2

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E0588h

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96T

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ELISA Kit for Rat Pyruvate kinase isozymes M1/M2

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E0588r

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96T

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ELISA Kit for Rat Pyruvate kinase isozymes M1/M2

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E0588m

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ELISA Kit for Rat Pyruvate kinase isozymes M1/M2

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ELISA Kit for Rat Pyruvate kinase isozymes M1/M2

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E0588c

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96T

CLIA Kit for Rat Pyruvate kinase isozymes M1/M2

Cat.:

U0588r

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96T

CLIA Kit for Rat Pyruvate kinase isozymes M1/M2

Cat.:

U0588h

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96T

CLIA Kit for Rat Pyruvate kinase isozymes M1/M2

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CLIA Kit for Rat Pyruvate kinase isozymes M1/M2

Cat.:

U0588m

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96T

CLIA Kit for Rat Pyruvate kinase isozymes M1/M2

Cat.:

U0588c

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96T

Polyclonal Antibody for Rat Pyruvate kinase isozymes M1/M2

Cat.:

P0588Rb-r

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40ug/0.2ml

Polyclonal Antibody for Rat Pyruvate kinase isozymes M1/M2

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P0588Rb-h

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Polyclonal Antibody for Rat Pyruvate kinase isozymes M1/M2

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P0588Rb-m

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Monoclonal Antibody for Rat Pyruvate kinase isozymes M1/M2

Protein for Rat Pyruvate kinase isozymes M1/M2

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"The M1- and M2-type isozymes of rat pyruvate kinase are produced from the same gene by alternative RNA splicing."
Noguchi T. , Inoue H. , Tanaka T.
J. Biol. Chem.261:13807-13812(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS M1 AND M2)
2.

"Rat pyruvate kinase M gene: its complete structure and characterization of the 5'-flanking region."
Takenaka M. , Noguchi T. , Inoue H. , Yamada K. , Matsuda T. , Tanaka T.
J. Biol. Chem.264:2363-2367(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS M1 AND M2);SEQUENCE REVISION
3.

"The nucleotide sequence of a full length cDNA encoding rat pituitary pyruvate kinase."
Parkinson C. , Kato H. , Cheng S.
Nucleic Acids Res.17:7106-7106(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM M1)
tissue: Pituitary.
4.

Lubec G. , Afjehi-Sadat L. , Chen W.-Q. , Kang S.U.
Submitted (2007-07) to the UniProtKB

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 33-56; 74-89; 93-115; 174-186; 208-246; 279-294; 343-376; 393-400; 423-436; 476-498 AND 505-526;PROTEIN SEQUENCE OF 384-399 (ISOFORM M2);IDENTIFICATION BY MASS SPECTROMETRY
strain: Sprague-Dawley.
tissue: Brain.
tissue: Hippocampus.
tissue: Spinal cord.

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