E-selectin (Protein name
), LYAM2_BOVIN from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Related Databases
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Gene name:
SELE;
Protein name:
E-selectin;
Alternative:
Endothelial leukocyte adhesion molecule 1(ELAM-1);CD62 antigen-like family member E;Leukocyte-endothelial cell adhesion molecule 2(LECAM2);
CD:
CD62E;
Organism:
Bovine (Bos taurus).
General Annotation
Sub Unit:
Interacts with PSGL1/SELPLG and PODXL2 through the sialyl Lewis X epitope. PSGL1 sulfation appears not to be required for this interaction.
Function:
Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.1%
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Recovery
Recovery was determined by spiking various levels of ELAM1 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
97
95-104
Plasma
98
93-99
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ELAM1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
92-95%
85-89%
95-100%
93-98%
EDTA plasma(n=5)
98-105%
99-104%
92-96%
91-98%
heparin plasma(n=5)
89-93%
91-96%
95-104%
98-107%
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