MARK2 ELISA Kit for Human(Homo sapiens) | EIAab.comMARK2 ELISA Kit for Human(Homo sapiens)

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MARK2 (Gene name),

Serine/threonine-protein kinase MARK2 (Protein name ), MARK2_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

MARK2(EMK1);

Protein name:

Serine/threonine-protein kinase MARK2;

Alternative:

MAP/microtubule affinity-regulating kinase 2;ELKL motif kinase 1(EMK-1);PAR1 homolog b(Par-1b;Par1b);PAR1 homolog;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homodimer. Interacts with PAK7/PAK5; leading to inhibit the protein kinase activity (By similarity). Interacts (when phosphorylated at Thr-596) with YWHAZ. In case of infection, interacts with H.pylori CagA, leading to inhibit kinase activity and junctional and polarity defects.

Function:

Serine/threonine-protein kinase involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4, MAPT/TAU, and RAB11FIP2. Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule-dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells.

Subcellular Location:

Cell membrane Peripheral membrane protein Cytoplasm Phosphorylation at Thr-596 by PRKCZ/aPKC and subsequent interaction with 14-3-3 protein YWHAZ promotes relocation from the cell membrane to the cytoplasm.

Protein Attributes:

Sequence length:

788

Sequence:

50:

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300:

350:

400:

450:

500:

550:

600:

650:

700:

750:

788:

EVCKLPRLSL | NGVRFKRISG | TSMAFKNIAS | KIANELKL

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

Q7KZI7

UniGene:

Hs.567261

MIM:

600526

Pfam:

PF02149

KEGG:

hsa:2011

STRING:

9606.ENSP00000389184

SMR:

Q7KZI7

Uniprot:

Q7KZI7

FOR

ELISA Kit for Human Serine/threonine-protein kinase MARK2

CLIA Kit for Human Serine/threonine-protein kinase MARK2

Polyclonal Antibody for Human Serine/threonine-protein kinase MARK2

Monoclonal Antibody for Human Serine/threonine-protein kinase MARK2

Protein for Human Serine/threonine-protein kinase MARK2

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Human serine/threonine protein kinase EMK1: genome structure and cDNA cloning of isoforms produced by alternative splicing."
Espinosa L. , Navarro E.
Cytogenet. Cell Genet.81:278-282(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3);TISSUE SPECIFICITY
tissue: Colon.
2.

Sugiyama A. , Inoue H. , Oka M.
Submitted (2004-08) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5)
tissue: Kidney.
3.

"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
Submitted (2003-05) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 6)
4.

"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D. , Noguchi H. , Totoki Y. , Toyoda A. , Kuroki Y. , Dewar K. , Lloyd C. , Itoh T. , Takeda T. , Kim D.-W. , She X. , Barlow K.F. , Bloom T. , Bruford E. , Chang J.L. , Cuomo C.A. , Eichler E. , FitzGerald M.G. , more...
Nature440:497-500(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
5.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4);NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-755 (ISOFORM 1)
tissue: Kidney.
tissue: Placenta.
6.

"PAR-1 is a Dishevelled-associated kinase and a positive regulator of Wnt signalling."
Sun T.-Q. , Lu B. , Feng J.-J. , Reinhard C. , Jan Y.N. , Fantl W.J. , Williams L.T.
Nat. Cell Biol.3:628-636(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 28-788 (ISOFORM 4);FUNCTION
7.

"Identification of 21 novel human protein kinases, including 3 members of a family related to the cell cycle regulator nimA of Aspergillus nidulans."
Schultz S.J. , Nigg E.A.
Cell Growth Differ.4:821-830(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 190-237
8.

"Protein kinase MARK/PAR-1 is required for neurite outgrowth and establishment of neuronal polarity."
Biernat J. , Wu Y.Z. , Timm T. , Zheng-Fischhofer Q. , Mandelkow E. , Meijer L. , Mandelkow E.M.
Mol. Biol. Cell13:4013-4028(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;ENZYME REGULATION
9.

"Splicing alterations in human renal allografts: detection of a new splice variant of protein kinase Par1/Emk1 whose expression is associated with an increase of inflammation in protocol biopsies of transplanted patients."
Hueso M. , Beltran V. , Moreso F. , Ciriero E. , Fulladosa X. , Grinyo J.M. , Seron D. , Navarro E.
Biochim. Biophys. Acta1689:58-65(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;ALTERNATIVE PROMOTER USAGE
10.

"LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1."
Lizcano J.M. , Goeransson O. , Toth R. , Deak M. , Morrice N.A. , Boudeau J. , Hawley S.A. , Udd L. , Maekelae T.P. , Hardie D.G. , Alessi D.R.
EMBO J.23:833-843(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;ENZYME REGULATION;PHOSPHORYLATION AT THR-208;MUTAGENESIS OF THR-208
11.

"Par-1 promotes a hepatic mode of apical protein trafficking in MDCK cells."
Cohen D. , Rodriguez-Boulan E. , Musch A.
Proc. Natl. Acad. Sci. U.S.A.101:13792-13797(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
12.

"Atypical PKC phosphorylates PAR-1 kinases to regulate localization and activity."
Hurov J.B. , Watkins J.L. , Piwnica-Worms H.
Curr. Biol.14:736-741(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-596;MUTAGENESIS OF THR-596;SUBCELLULAR LOCATION
13.

"aPKC acts upstream of PAR-1b in both the establishment and maintenance of mammalian epithelial polarity."
Suzuki A. , Hirata M. , Kamimura K. , Maniwa R. , Yamanaka T. , Mizuno K. , Kishikawa M. , Hirose H. , Amano Y. , Izumi N. , Miwa Y. , Ohno S.
Curr. Biol.14:1425-1435(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;PHOSPHORYLATION AT THR-596;MUTAGENESIS OF THR-596;INTERACTION WITH YWHAZ
14.

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V. , Blagoev B. , Gnad F. , Macek B. , Kumar C. , Mortensen P. , Mann M.
Cell127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-486;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
15.

"MARK2/EMK1/Par-1Balpha phosphorylation of Rab11-family interacting protein 2 is necessary for the timely establishment of polarity in Madin-Darby canine kidney cells."
Ducharme N.A. , Hales C.M. , Lapierre L.A. , Ham A.J. , Oztan A. , Apodaca G. , Goldenring J.R.
Mol. Biol. Cell17:3625-3637(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF RAB11FIP2
16.

"New role for hPar-1 kinases EMK and C-TAK1 in regulating localization and activity of class IIa histone deacetylases."
Dequiedt F. , Martin M. , Von Blume J. , Vertommen D. , Lecomte E. , Mari N. , Heinen M.F. , Bachmann M. , Twizere J.C. , Huang M.C. , Rider M.H. , Piwnica-Worms H. , Seufferlein T. , Kettmann R.
Mol. Cell. Biol.26:7086-7102(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF HDAC7
17.

"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A. , Villen J. , Gerber S.A. , Rush J. , Gygi S.P.
Nat. Biotechnol.24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-456;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
18.

"Helicobacter pylori CagA targets PAR1/MARK kinase to disrupt epithelial cell polarity."
Saadat I. , Higashi H. , Obuse C. , Umeda M. , Murata-Kamiya N. , Saito Y. , Lu H. , Ohnishi N. , Azuma T. , Suzuki A. , Ohno S. , Hatakeyama M.
Nature447:330-333(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH H.PYLORI CAGA
19.

"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T. , Yi W. , Lu B. , Park S.K. , Xu T. , Lee J.-D. , Yates J.R. III
J. Proteome Res.7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-456 AND SER-486;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
20.

"Phosphoproteome of resting human platelets."
Zahedi R.P. , Lewandrowski U. , Wiesner J. , Wortelkamp S. , Moebius J. , Schuetz C. , Walter U. , Gambaryan S. , Sickmann A.
J. Proteome Res.7:526-534(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-456;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Platelet.
21.

"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H. , Olsen J.V. , Bairlein M. , Gnad F. , Oppermann F.S. , Korner R. , Greff Z. , Keri G. , Stemmann O. , Mann M.
Mol. Cell31:438-448(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-409; SER-456; SER-486 AND SER-493;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
22.

"Glucose controls CREB activity in islet cells via regulated phosphorylation of TORC2."
Jansson D. , Ng A.C. , Fu A. , Depatie C. , Al Azzabi M. , Screaton R.A.
Proc. Natl. Acad. Sci. U.S.A.105:10161-10166(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF CRTC2
23.

"The ubiquitin-associated domain of AMPK-related kinases regulates conformation and LKB1-mediated phosphorylation and activation."
Jaleel M. , Villa F. , Deak M. , Toth R. , Prescott A.R. , Van Aalten D.M. , Alessi D.R.
Biochem. J.394:545-555(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DOMAIN UBA
24.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-569;PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-376 (ISOFORMS 13 AND 14);PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-409 (ISOFORMS 15 AND 16);IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
25.

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
26.

"Large-scale proteomics analysis of the human kinome."
Oppermann F.S. , Gnad F. , Olsen J.V. , Hornberger R. , Greff Z. , Keri G. , Mann M. , Daub H.
Mol. Cell. Proteomics8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-208; SER-409; SER-456; SER-486 AND SER-722;PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-376 (ISOFORMS 13 AND 14);IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
27.

"Par1b/MARK2 phosphorylates kinesin-like motor protein GAKIN/KIF13B to regulate axon formation."
Yoshimura Y. , Terabayashi T. , Miki H.
Mol. Cell. Biol.30:2206-2219(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF KIF13B
28.

"The tau of MARK: a polarized view of the cytoskeleton."
Matenia D. , Mandelkow E.M.
Trends Biochem. Sci.34:332-342(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
29.

"Structure and function of polarity-inducing kinase family MARK/Par-1 within the branch of AMPK/Snf1-related kinases."
Marx A. , Nugoor C. , Panneerselvam S. , Mandelkow E.
FASEB J.24:1637-1648(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
30.

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
31.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
32.

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T. , Prokhorova T.A. , Akimov V. , Henningsen J. , Johansen P.T. , Kratchmarova I. , Kassem M. , Mann M. , Olsen J.V. , Blagoev B.
Sci. Signal.4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-40; SER-486 AND SER-619;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
33.

"The novel PAR-1-binding protein MTCL1 has crucial roles in organizing microtubules in polarizing epithelial cells."
Sato Y. , Akitsu M. , Amano Y. , Yamashita K. , Ide M. , Shimada K. , Yamashita A. , Hirano H. , Arakawa N. , Maki T. , Hayashi I. , Ohno S. , Suzuki A.
J. Cell Sci.126:4671-4683(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH MTCL1;SUBCELLULAR LOCATION
34.

"Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates."
Nesic D. , Miller M.C. , Quinkert Z.T. , Stein M. , Chait B.T. , Stebbins C.E.
Nat. Struct. Mol. Biol.17:130-132(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 49-363 IN COMPLEX WITH H.PYLORI CAGA PEPTIDE INHIBITOR

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