MAD1L1 ELISA Kit for Human(Homo sapiens) | EIAab.comMAD1L1 ELISA Kit for Human(Homo sapiens)

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MAD1L1 (Gene name),

Mitotic spindle assembly checkpoint protein MAD1 (Protein name ), MD1L1_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

MAD1L1(MAD1;TXBP181);

Protein name:

Mitotic spindle assembly checkpoint protein MAD1;

Alternative:

Mitotic checkpoint MAD1 protein homolog(HsMAD1;hMAD1);Mitotic arrest deficient 1-like protein 1(MAD1-like protein 1);Tax-binding protein 181;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homodimer. Heterodimerizes with MAD2L1 in order to form a tetrameric MAD1L1-MAD2L1 core complex. Perturbation of the original MAD1L1-MAD2L1 structure by the spindle checkpoint may decrease MAD2L1 affinity for MAD1L1. CDC20 can compete with MAD1L1 for MAD2L1 binding, until the attachment and/or tension dampen the checkpoint signal, preventing further release of MAD2L1 on to CDC20. Also able to interact with the BUB1/BUB3 complex and the viral Tax protein. Interacts with TPR and NEK2.

Function:

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.

Subcellular Location:

Nucleus Chromosome centromere kinetochore Cytoplasm cytoskeleton centrosome Cytoplasm cytoskeleton spindle From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Co-localizes with NEK2 at the kinetochore.

Protein Attributes:

Sequence length:

718

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

718:

PAFLSSLTLE | LFSRQTVA

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Hs.654838

KEGG:

hsa:8379

Pfam:

PF05557

STRING:

9606.ENSP00000382562

SMR:

Q9Y6D9

String:

Q9Y6D9

MIM:

602686

Uniprot:

Q9Y6D9

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ELISA Kit for Human Mitotic spindle assembly checkpoint protein MAD1

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E5695h

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96T

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ELISA Kit for Human Mitotic spindle assembly checkpoint protein MAD1

Cat.:

E5695m

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Packing:

96T

CLIA Kit for Human Mitotic spindle assembly checkpoint protein MAD1

Cat.:

U5695h

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MSDS:

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Packing:

96T

CLIA Kit for Human Mitotic spindle assembly checkpoint protein MAD1

Cat.:

U5695m

Price:

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MSDS:

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Packing:

96T

Polyclonal Antibody for Human Mitotic spindle assembly checkpoint protein MAD1

Cat.:

P5695Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human Mitotic spindle assembly checkpoint protein MAD1

Cat.:

P5695Rb-h

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human Mitotic spindle assembly checkpoint protein MAD1

Protein for Human Mitotic spindle assembly checkpoint protein MAD1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Human T cell leukemia virus type 1 oncoprotein Tax targets the human mitotic checkpoint protein MAD1."
Jin D.-Y. , Spencer F. , Jeang K.-T.
Cell93:81-91(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);HOMODIMERIZATION;HETEROTETRAMERIZATION;SUBCELLULAR LOCATION;PHOSPHORYLATION;INTERACTION WITH MAD2L1 AND VIRAL TAX
2.

"Phosphorylation of human MAD1 by the BUB1 kinase in vitro."
Seeley T.W. , Wang L. , Zhen J.Y.
Biochem. Biophys. Res. Commun.257:589-595(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);PHOSPHORYLATION BY BUB1;INTERACTION WITH BUB1/BUB3 COMPLEX
tissue: Testis.
3.

"Mitotic checkpoint locus MAD1L1 maps to human chromosome 7p22 and mouse chromosome 5."
Jin D.-Y. , Kozak C.A. , Pangilinan F. , Spencer F. , Green E.D. , Jeang K.-T.
Genomics55:363-364(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);INDUCTION;FUNCTION
4.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
tissue: Amygdala.
5.

"The DNA sequence of human chromosome 7."
Hillier L.W. , Fulton R.S. , Fulton L.A. , Graves T.A. , Pepin K.H. , Wagner-McPherson C. , Layman D. , Maas J. , Jaeger S. , Walker R. , Wylie K. , Sekhon M. , Becker M.C. , O'Laughlin M.D. , Schaller M.E. , Fewell G.A. , Delehaunty K.D. , Miner T.L. , more...
Nature424:157-164(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
6.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
tissue: Pancreas.
7.

"Multiple tumor suppressor pathways negatively regulate telomerase."
Lin S.Y. , Elledge S.J.
Cell113:881-889(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
8.

"NEK2A interacts with MAD1 and possibly functions as a novel integrator of the spindle checkpoint signaling."
Lou Y. , Yao J. , Zereshki A. , Dou Z. , Ahmed K. , Wang H. , Hu J. , Wang Y. , Yao X.
J. Biol. Chem.279:20049-20057(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;INTERACTION WITH NEK2
9.

"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A. , Villen J. , Gerber S.A. , Rush J. , Gygi S.P.
Nat. Biotechnol.24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-428;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
10.

"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S. , Ballif B.A. , Smogorzewska A. , McDonald E.R. III , Hurov K.E. , Luo J. , Bakalarski C.E. , Zhao Z. , Solimini N. , Lerenthal Y. , Shiloh Y. , Gygi S.P. , Elledge S.J.
Science316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-214;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Embryonic kidney.
11.

"Tpr directly binds to Mad1 and Mad2 and is important for the Mad1-Mad2-mediated mitotic spindle checkpoint."
Lee S.H. , Sterling H. , Burlingame A. , McCormick F.
Genes Dev.22:2926-2931(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TPR AND MAD2L1;IDENTIFICATION BY MASS SPECTROMETRY;SUBCELLULAR LOCATION
12.

"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H. , Olsen J.V. , Bairlein M. , Gnad F. , Oppermann F.S. , Korner R. , Greff Z. , Keri G. , Stemmann O. , Mann M.
Mol. Cell31:438-448(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
13.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16 AND SER-428;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
14.

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
15.

"Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator."
Lince-Faria M. , Maffini S. , Orr B. , Ding Y. , Florindo C. , Sunkel C.E. , Tavares A. , Johansen J. , Johansen K.M. , Maiato H.
J. Cell Biol.184:647-657(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TPR
16.

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C. , Kumar C. , Gnad F. , Nielsen M.L. , Rehman M. , Walther T.C. , Olsen J.V. , Mann M.
Science325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-61;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
17.

"Nucleoporin translocated promoter region (Tpr) associates with dynein complex, preventing chromosome lagging formation during mitosis."
Nakano H. , Funasaka T. , Hashizume C. , Wong R.W.
J. Biol. Chem.285:10841-10849(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH TPR;SUBCELLULAR LOCATION
18.

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-428;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
19.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
20.

"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V. , Sumpton D. , Martinez A. , Lilla S. , Espagne C. , Meinnel T. , Giglione C.
Mol. Cell. Proteomics11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
21.

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P. , Lasa M. , Polevoda B. , Gazquez C. , Elosegui-Artola A. , Kim D.S. , De Juan-Pardo E. , Demeyer K. , Hole K. , Larrea E. , Timmerman E. , Prieto J. , Arnesen T. , Sherman F. , Gevaert K. , Aldabe R.
Proc. Natl. Acad. Sci. U.S.A.109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
22.

"Crystal structure of the tetrameric Mad1-Mad2 core complex: implications of a 'safety belt' binding mechanism for the spindle checkpoint."
Sironi L. , Mapelli M. , Knapp S. , De Antoni A. , Jeang K.-T. , Musacchio A.
EMBO J.21:2496-2506(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 493-579 IN COMPLEX WITH MAD2L1;COMPETITIVE INHIBITOR OF MAD2L1-CDC20 INTERACTION
23.

"Characterization of MAD2B and other mitotic spindle checkpoint genes."
Cahill D.P. , da Costa L.T. , Carson-Walter E.B. , Kinzler K.W. , Vogelstein B. , Lengauer C.
Genomics58:181-187(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS HIS-558 AND HIS-572
24.

"Search for in vivo somatic mutations in the mitotic checkpoint gene, hMAD1, in human lung cancers."
Nomoto S. , Haruki N. , Takahashi T. , Masuda A. , Koshikawa T. , Takahashi T. , Fujii Y. , Osada H. , Takahashi T.
Oncogene18:7180-7183(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT LUNG CANCER ALA-299;VARIANTS SER-160; MET-500; LYS-511; HIS-556 AND HIS-558
25.

"Mutations in the mitotic check point gene, MAD1L1, in human cancers."
Tsukasaki K. , Miller C.W. , Greenspun E. , Eshaghian S. , Kawabata H. , Fujimoto T. , Tomonaga M. , Sawyers C. , Said J.W. , Koeffler H.P.
Oncogene20:3301-3305(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS CANCER LEU-29; CYS-59; GLN-360; LYS-516; CYS-556 AND LYS-569;VARIANTS MET-500 AND HIS-558

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