Gene name:
WDR77 (HKMT1069;Nbla10071;Nbla10071;MEP50;WD45) ;
Protein name:
Methylosome protein 50 (MEP-50) ;
Alternative:
WD repeat-containing protein 77 ;Androgen receptor cofactor p44 ;p44/Mep50 ;
Organism:
Human (Homo sapiens).
General Annotation
Sub Unit:
Component of the methylosome, a 20S complex containing at least PRMT5, CLNS1A and WDR77. Directly interacts with PRMT5, as well as with several Sm proteins, including SNRPB and SNRPD2 and, more weakly, SNRPD3 and SNRPE. Interacts with SUZ12 and histone H2A/HIST2H2AC, but not with histones H2B, H3 nor H4. Interacts with CTDP1 and LSM11. Interacts with APEX1, AR and NKX3-1.
Function:
Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.
Subcellular Location:
Nucleus
Cytoplasm
Nuclear in Leydig cells and cytoplasmic in germ cells during fetal testicular development. In adult testis, predominantly nuclear. Subcellular location varies from nuclear to cytoplasmic in various tumors.
Protein Attributes:
50:
MRKETPPPLV | PPAAREWNLP | PNAPACMERQ | LEAARYRSDG | ALLLGASSLS |
100:
GRCWAGSLWL | FKDPCAAPNE | GFCSAGVQTE | AGVADLTWVG | ERGILVASDS |
150:
GAVELWELDE | NETLIVSKFC | KYEHDDIVST | VSVLSSGTQA | VSGSKDICIK |
200:
VWDLAQQVVL | SSYRAHAAQV | TCVAASPHKD | SVFLSCSEDN | RILLWDTRCP |
250:
KPASQIGCSA | PGYLPTSLAW | HPQQSEVFVF | GDENGTVSLV | DTKSTSCVLS |
300:
SAVHSQCVTG | LVFSPHSVPF | LASLSEDCSL | AVLDSSLSEL | FRSQAHRDFV |
342:
RDATWSPLNH | SLLTTVGWDH | QVVHHVVPTE | PLPAPGPASV | TE
Vaild Sequence:
Related Databases
Uniprot:
ELISA Kit
CLIA Kit
Polyclonal Antibody
Monoclonal Antibody
Protein
FOR
Mouse
Rat
Bovine
Human
ELISA Kit for Human MEP-50
ELISA Kit for Human MEP-50
ELISA Kit for Human MEP-50
ELISA Kit for Human MEP-50
CLIA Kit for Human MEP-50
CLIA Kit for Human MEP-50
CLIA Kit for Human MEP-50
CLIA Kit for Human MEP-50
Polyclonal Antibody for Human MEP-50
Polyclonal Antibody for Human MEP-50
Polyclonal Antibody for Human MEP-50
Polyclonal Antibody for Human MEP-50
Monoclonal Antibody for Human MEP-50
Monoclonal Antibody for Human MEP-50
Monoclonal Antibody for Human MEP-50
Monoclonal Antibody for Human MEP-50
R&D Technical Data
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Precision
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Recovery
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Linearity
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References
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Cited for : NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);PROTEIN SEQUENCE OF 4-15; 38-52; 122-145; 151-164 AND 192-198;FUNCTION;INTERACTION WITH PRMT5; SNRPB; SNRPD2; SNRPD3 AND SNRPE;IDENTIFICATION BY MASS SPECTROMETRY
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);PROTEIN SEQUENCE OF 3-15;TISSUE SPECIFICITY;INTERACTION WITH AR AND NKX3-1
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[
PubMed ]
[
Europe PMC ]
[
Abstract ]
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Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2)
tissue :
Kidney proximal tubule .
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[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
tissue :
Hepatoblastoma .
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
6.
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more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases
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Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
7.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
tissue :
Colon .
tissue :
Lung .
tissue :
Skin .
8.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PROTEIN SEQUENCE OF 3-15; 36-52; 122-145; 151-179 AND 192-198;PHOSPHORYLATION AT THR-5;IDENTIFICATION BY MASS SPECTROMETRY
tissue :
Colon carcinoma .
9.
"Neuroblastoma oligo-capping cDNA project: toward the understanding of the genesis and biology of neuroblastoma."
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Morohashi A.
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Cancer Lett.197:63-68(2003)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 184-342 (ISOFORM 1)
tissue :
Neuroblastoma .
10.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : SUBCELLULAR LOCATION;INTERACTION WITH CTDP1
11.
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Cited for : INTERACTION WITH LSM11
12.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : SUBCELLULAR LOCATION;INTERACTION WITH HIST2H2AC; PRMT5 AND SUZ12
13.
"The expression and function of androgen receptor coactivator p44 and protein arginine methyltransferase 5 in the developing testis and testicular tumors."
Liang J.J.
,
Wang Z.
,
Chiriboga L.
,
Greco M.A.
,
Shapiro E.
,
Huang H.
,
Yang X.J.
,
Huang J.
,
Peng Y.
,
Melamed J.
,
Garabedian M.J.
,
Lee P.
J. Urol.177:1918-1922(2007)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
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Cited for : SUBCELLULAR LOCATION;TISSUE SPECIFICITY;DEVELOPMENTAL STAGE
14.
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Cited for : IDENTIFICATION IN THE METHYLOSOME COMPLEX
15.
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Cited for : IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Cervix carcinoma .
16.
"APE1/Ref-1 interacts with NPM1 within nucleoli and plays a role in the rRNA quality control process."
Vascotto C.
,
Fantini D.
,
Romanello M.
,
Cesaratto L.
,
Deganuto M.
,
Leonardi A.
,
Radicella J.P.
,
Kelley M.R.
,
D'Ambrosio C.
,
Scaloni A.
,
Quadrifoglio F.
,
Tell G.
Mol. Cell. Biol.29:1834-1854(2009)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
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Cited for : INTERACTION WITH APEX1;IDENTIFICATION BY MASS SPECTROMETRY;SUBCELLULAR LOCATION
17.
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Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-5;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Leukemic T-cell .
18.
"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V.
,
Vermeulen M.
,
Santamaria A.
,
Kumar C.
,
Miller M.L.
,
Jensen L.J.
,
Gnad F.
,
Cox J.
,
Jensen T.S.
,
Nigg E.A.
,
Brunak S.
,
Mann M.
Sci. Signal.3:RA3-RA3(2010)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-5;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Cervix carcinoma .
19.
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Cited for : IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
20.
"Crystal structure of the human PRMT5:MEP50 complex."
Antonysamy S.
,
Bonday Z.
,
Campbell R.M.
,
Doyle B.
,
Druzina Z.
,
Gheyi T.
,
Han B.
,
Jungheim L.N.
,
Qian Y.
,
Rauch C.
,
Russell M.
,
Sauder J.M.
,
Wasserman S.R.
,
Weichert K.
,
Willard F.S.
,
Zhang A.
,
Emtage S.
Proc. Natl. Acad. Sci. U.S.A.109:17960-17965(2012)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
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Cited for : X-RAY CRYSTALLOGRAPHY (2.06 ANGSTROMS) OF 2-342 IN COMPLEX WITH PRMT5;FUNCTION;WD REPEATS;SUBUNIT