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Index > Protein center > WDR77(Gene name) > Human
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  • WDR77 (Gene name),
  • Methylosome protein 50 (Protein name ),  MEP50_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Gene name:
    WDR77(HKMT1069;Nbla10071;Nbla10071;MEP50;WD45);
    Protein name:
    Methylosome protein 50(MEP-50);
    Alternative:
    WD repeat-containing protein 77;Androgen receptor cofactor p44;p44/Mep50;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Component of the methylosome, a 20S complex containing at least PRMT5, CLNS1A and WDR77. Directly interacts with PRMT5, as well as with several Sm proteins, including SNRPB and SNRPD2 and, more weakly, SNRPD3 and SNRPE. Interacts with SUZ12 and histone H2A/HIST2H2AC, but not with histones H2B, H3 nor H4. Interacts with CTDP1 and LSM11. Interacts with APEX1, AR and NKX3-1.
    Function:
    Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.
    Subcellular Location:
    Nucleus Cytoplasm Nuclear in Leydig cells and cytoplasmic in germ cells during fetal testicular development. In adult testis, predominantly nuclear. Subcellular location varies from nuclear to cytoplasmic in various tumors.
    Protein Attributes:
    Sequence length:
    342
    Sequence:
    50:
    MRKETPPPLV | PPAAREWNLP | PNAPACMERQ | LEAARYRSDG | ALLLGASSLS | 
    100:
    GRCWAGSLWL | FKDPCAAPNE | GFCSAGVQTE | AGVADLTWVG | ERGILVASDS | 
    150:
    GAVELWELDE | NETLIVSKFC | KYEHDDIVST | VSVLSSGTQA | VSGSKDICIK | 
    200:
    VWDLAQQVVL | SSYRAHAAQV | TCVAASPHKD | SVFLSCSEDN | RILLWDTRCP | 
    250:
    KPASQIGCSA | PGYLPTSLAW | HPQQSEVFVF | GDENGTVSLV | DTKSTSCVLS | 
    300:
    SAVHSQCVTG | LVFSPHSVPF | LASLSEDCSL | AVLDSSLSEL | FRSQAHRDFV | 
    342:
    RDATWSPLNH | SLLTTVGWDH | QVVHHVVPTE | PLPAPGPASV | TE
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    SMR:
    UniGene:
    MIM:
    KEGG:
    Pfam:
    String:
    Uniprot:
     
    FOR
    ELISA Kit for Human MEP-50
    ELISA Kit for Human MEP-50
    ELISA Kit for Human MEP-50
    ELISA Kit for Human MEP-50
    CLIA Kit for Human MEP-50
    CLIA Kit for Human MEP-50
    CLIA Kit for Human MEP-50
    CLIA Kit for Human MEP-50
    Polyclonal Antibody for Human MEP-50
    Polyclonal Antibody for Human MEP-50
    Polyclonal Antibody for Human MEP-50
    Polyclonal Antibody for Human MEP-50
    Monoclonal Antibody for Human MEP-50
    Monoclonal Antibody for Human MEP-50
    Monoclonal Antibody for Human MEP-50
    Monoclonal Antibody for Human MEP-50
    Protein for Human MEP-50
    Protein for Human MEP-50
    Protein for Human MEP-50
    Protein for Human MEP-50

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    References
    1. 1.
      "A novel WD repeat protein component of the methylosome binds Sm proteins."
      Friesen W.J. , Wyce A. , Paushkin S. , Abel L. , Rappsilber J. , Mann M. , Dreyfuss G.
      J. Biol. Chem.277:8243-8247(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);PROTEIN SEQUENCE OF 4-15; 38-52; 122-145; 151-164 AND 192-198;FUNCTION;INTERACTION WITH PRMT5; SNRPB; SNRPD2; SNRPD3 AND SNRPE;IDENTIFICATION BY MASS SPECTROMETRY
    2. 2.
      "Purification and identification of a novel complex which is involved in androgen receptor-dependent transcription."
      Hosohata K. , Li P. , Hosohata Y. , Qin J. , Roeder R.G. , Wang Z.
      Mol. Cell. Biol.23:7019-7029(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);PROTEIN SEQUENCE OF 3-15;TISSUE SPECIFICITY;INTERACTION WITH AR AND NKX3-1
    3. 3.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2)
      tissue: Kidney proximal tubule.
    4. 4.
      "Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas."
      Yamada S. , Ohira M. , Horie H. , Ando K. , Takayasu H. , Suzuki Y. , Sugano S. , Hirata T. , Goto T. , Matsunaga T. , Hiyama E. , Hayashi Y. , Ando H. , Suita S. , Kaneko M. , Sasaki F. , Hashizume K. , Ohnuma N. , more...
      Oncogene23:5901-5911(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      tissue: Hepatoblastoma.
    5. 5.
      "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G. , Barlow K.F. , McLay K.E. , Kaul R. , Swarbreck D. , Dunham A. , Scott C.E. , Howe K.L. , Woodfine K. , Spencer C.C.A. , Jones M.C. , Gillson C. , Searle S. , Zhou Y. , Kokocinski F. , McDonald L. , Evans R. , Phillips K. , more...
      Nature441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    6. 6.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    7. 7.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      tissue: Colon.
      tissue: Lung.
      tissue: Skin.
    8. 8.
      Bienvenut W.V. , Zebisch A. , Kolch W.
      Submitted (2008-12) to the UniProtKB
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 3-15; 36-52; 122-145; 151-179 AND 192-198;PHOSPHORYLATION AT THR-5;IDENTIFICATION BY MASS SPECTROMETRY
      tissue: Colon carcinoma.
    9. 9.
      "Neuroblastoma oligo-capping cDNA project: toward the understanding of the genesis and biology of neuroblastoma."
      Ohira M. , Morohashi A. , Nakamura Y. , Isogai E. , Furuya K. , Hamano S. , Machida T. , Aoyama M. , Fukumura M. , Miyazaki K. , Suzuki Y. , Sugano S. , Hirato J. , Nakagawara A.
      Cancer Lett.197:63-68(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 184-342 (ISOFORM 1)
      tissue: Neuroblastoma.
    10. 10.
      "The FCP1 phosphatase interacts with RNA polymerase II and with MEP50 a component of the methylosome complex involved in the assembly of snRNP."
      Licciardo P. , Amente S. , Ruggiero L. , Monti M. , Pucci P. , Lania L. , Majello B.
      Nucleic Acids Res.31:999-1005(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;INTERACTION WITH CTDP1
    11. 11.
      "Toward an assembly line for U7 snRNPs: interactions of U7-specific Lsm proteins with PRMT5 and SMN complexes."
      Azzouz T.N. , Pillai R.S. , Dapp C. , Chari A. , Meister G. , Kambach C. , Fischer U. , Schuemperli D.
      J. Biol. Chem.280:34435-34440(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH LSM11
    12. 12.
      "Association of Polycomb group SUZ12 with WD-repeat protein MEP50 that binds to histone H2A selectively in vitro."
      Furuno K. , Masatsugu T. , Sonoda M. , Sasazuki T. , Yamamoto K.
      Biochem. Biophys. Res. Commun.345:1051-1058(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;INTERACTION WITH HIST2H2AC; PRMT5 AND SUZ12
    13. 13.
      "The expression and function of androgen receptor coactivator p44 and protein arginine methyltransferase 5 in the developing testis and testicular tumors."
      Liang J.J. , Wang Z. , Chiriboga L. , Greco M.A. , Shapiro E. , Huang H. , Yang X.J. , Huang J. , Peng Y. , Melamed J. , Garabedian M.J. , Lee P.
      J. Urol.177:1918-1922(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;TISSUE SPECIFICITY;DEVELOPMENTAL STAGE
    14. 14.
      "An assembly chaperone collaborates with the SMN complex to generate spliceosomal SnRNPs."
      Chari A. , Golas M.M. , Klingenhager M. , Neuenkirchen N. , Sander B. , Englbrecht C. , Sickmann A. , Stark H. , Fischer U.
      Cell135:497-509(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION IN THE METHYLOSOME COMPLEX
    15. 15.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    16. 16.
      "APE1/Ref-1 interacts with NPM1 within nucleoli and plays a role in the rRNA quality control process."
      Vascotto C. , Fantini D. , Romanello M. , Cesaratto L. , Deganuto M. , Leonardi A. , Radicella J.P. , Kelley M.R. , D'Ambrosio C. , Scaloni A. , Quadrifoglio F. , Tell G.
      Mol. Cell. Biol.29:1834-1854(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH APEX1;IDENTIFICATION BY MASS SPECTROMETRY;SUBCELLULAR LOCATION
    17. 17.
      "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
      Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-5;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Leukemic T-cell.
    18. 18.
      "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
      Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-5;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    19. 19.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    20. 20.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.06 ANGSTROMS) OF 2-342 IN COMPLEX WITH PRMT5;FUNCTION;WD REPEATS;SUBUNIT
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