Nr2c1 ELISA Kit for Mouse(Mus musculus) | EIAab.comNr2c1 ELISA Kit for Mouse(Mus musculus) | EIAab.com

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Nr2c1 (Gene name),

Nuclear receptor subfamily 2 group C member 1 (Protein name ), NR2C1_MOUSE from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Gene name:

Nr2c1(Tr2;Tr2-11);

Protein name:

Nuclear receptor subfamily 2 group C member 1;

Alternative:

Testicular receptor 2(mTR2);Orphan nuclear receptor TR2;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Homodimer. Heterodimer; with NR2C2 which is required for chromatin remodeling and for binding to promoter regions such as globin DR1 repeats. Interacts with ESR1; the interaction prevents homodimerization of ESR1 and suppresses its transcriptional activity and cell growth (By similarity). Interacts with NRIP1 (via its LXXLL motifs); the interaction provides corepressor activity. Interacts with HDAC3 (via the DNA-binding domain); the interaction recruits phosphorylated NR2C1 to PML bodies for sumoylation. Interacts with HDAC4 (via the DNA-binding domain). Interacts with PIAS1; the interaction is required for sumoylation of NR2C1. Interacts with UBE2I; the interaction is required for sumoylation of NR2C1. Interacts with KAT2B; the interaction acts as a corepressor of gene expression.

Function:

Orphan nuclear receptor. Binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. Primarily repressor of a broad range of genes including ESR1 and RARB. Together with NR2C2, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences (By similarity). Also activator of OCT4 gene expression. Plays a fundamental role in early embryogenesis and regulates embryonic stem cell proliferation and differentiation. Mediator of retinoic acid-regulated preadipocyte proliferation.

Subcellular Location:

Nucleus Nucleus PML body Recruited by HDAC3, after all-trans retinoic acid stimulated MAPK1-mediated Thr-210 phosphorylation, to PML bodies for subsequent sumoylation.

Protein Attributes:

Sequence length:

590

Sequence:

50:

MATIEEIAHQ | IIDQQMGEIV | TEQQTGQKIQ | IVTALDHSTQ | GKQFILANHE |

100:

GSTPGKVFLT | TPDAAGVNQL | FFTSPDLSAP | HLQLLTEKSP | DQGPNKVFDL |

150:

CVVCGDKASG | RHYGAITCEG | CKGFFKRSIR | KNLVYSCRGS | KDCVINKHHR |

200:

NRCQYCRLQR | CIAFGMKQDS | VQCERKPIEV | SREKSSNCAA | STEKIYIRKD |

250:

LRSPLAATPT | FVTDSETARS | AGLLDSGMFV | NIHPSGIKTE | PAMLMAPDKA |

300:

ESCQGDLSTL | ASVVTSLANL | GKAKDLSHCG | GDMPVVQSLR | NGDTSFGAFH |

350:

HDIQTNGDVS | RAFDTLAKAL | TPGESSACQS | PEEGMEGSPH | LIAGEPSFVE |

400:

KEGPLLSESH | VAFRLTMPSP | MPEYLNVHYI | GESASRLLFL | SMHWALSIPS |

450:

FQALGQENSI | SLVKAYWNEL | FTLGLAQCWQ | VMNVATILAT | FVNCLHSSLQ |

500:

QDKMSPERRK | SLMEHIFKLQ | EFCNSMVKLC | IDGHEYAYLK | AIVLFSPDHP |

550:

GLENMELIER | FQEKAYVEFQ | DYITRTYPDD | TYRLSRLLLR | LPALRLMNAT |

590:

ITEELFFKGL | IGNVRIDSVI | PHILKMEPAD | YNSQIIGHSL |

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

SMR:

Pfam:

UniGene:

String:

KEGG:

Uniprot:

FOR

ELISA Kit for Mouse Nuclear receptor subfamily 2 group C member 1

ELISA Kit for Mouse Nuclear receptor subfamily 2 group C member 1

ELISA Kit for Mouse Nuclear receptor subfamily 2 group C member 1

ELISA Kit for Mouse Nuclear receptor subfamily 2 group C member 1

CLIA Kit for Mouse Nuclear receptor subfamily 2 group C member 1

CLIA Kit for Mouse Nuclear receptor subfamily 2 group C member 1

CLIA Kit for Mouse Nuclear receptor subfamily 2 group C member 1

CLIA Kit for Mouse Nuclear receptor subfamily 2 group C member 1

Polyclonal Antibody for Mouse Nuclear receptor subfamily 2 group C member 1

Polyclonal Antibody for Mouse Nuclear receptor subfamily 2 group C member 1

Polyclonal Antibody for Mouse Nuclear receptor subfamily 2 group C member 1

Polyclonal Antibody for Mouse Nuclear receptor subfamily 2 group C member 1

Monoclonal Antibody for Mouse Nuclear receptor subfamily 2 group C member 1

Monoclonal Antibody for Mouse Nuclear receptor subfamily 2 group C member 1

Monoclonal Antibody for Mouse Nuclear receptor subfamily 2 group C member 1

Monoclonal Antibody for Mouse Nuclear receptor subfamily 2 group C member 1

Protein for Mouse Nuclear receptor subfamily 2 group C member 1

Protein for Mouse Nuclear receptor subfamily 2 group C member 1

Protein for Mouse Nuclear receptor subfamily 2 group C member 1

Protein for Mouse Nuclear receptor subfamily 2 group C member 1

R&D Technical Data

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Precision

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Recovery

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Linearity

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References

1.

"Genomic structure, promoter identification, and chromosomal mapping of a mouse nuclear orphan receptor expressed in embryos and adult testes."
Lee C.-H. , Copeland N.G. , Gilbert D.J. , Jenkins N.A. , Wei L.-N.
Genomics30:46-52(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];TISSUE SPECIFICITY;DEVELOPMENTAL STAGE;VARIANTS MET-29; MET-145; 296-SER--LYS-304; ILE-361; ALA-377 AND ALA-456
strain: CD-1.
tissue: Embryo.
2.

"Molecular cloning and characterization of a mouse nuclear orphan receptor expressed in embryos and testes."
Lee C.-H. , Chang L. , Wei L.N.
Mol. Reprod. Dev.44:305-314(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);FUNCTION;TISSUE SPECIFICITY;DEVELOPMENTAL STAGE;VARIANTS MET-29; MET-145; 296-SER--LYS-304; ILE-361; ALA-377 AND ALA-456
strain: CD-1.
tissue: Embryo.
3.

"Distinct expression patterns and biological activities of two isoforms of the mouse orphan receptor TR2."
Lee C.-H. , Chang L. , Wei L.-N.
J. Endocrinol.152:245-255(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2);FUNCTION;TISSUE SPECIFICITY;DEVELOPMENTAL STAGE
4.

"Polymorphism in the murine Tr2-11 gene encoding an orphan receptor, and its exclusion as a candidate gene for the cataract mutation Cat3."
Immervoll T. , Adamski J. , Graw J.
Biol. Chem.379:83-85(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);TISSUE SPECIFICITY
strain: C3H X 102.
5.

"A bidirectional regulation between the TR2/TR4 orphan receptors (TR2/TR4) and the ciliary neurotrophic factor (CNTF) signaling pathway."
Young W.-J. , Lee Y.-F. , Smith S.M. , Chang C.
J. Biol. Chem.273:20877-20885(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);TISSUE SPECIFICITY;INDUCTION
strain: C57BL/6.
tissue: Testis.
6.

"Cloning, antibody production and immunohistochemical localization of an androgen repressed of mouse and rat TR2 orphan receptors: a member of steroid receptor superfamily."
Ideta R. , Yeh S. , Lee Y.-F. , Adachi K. , Takeda H. , Su C. , Chang C.
Submitted (2001-11) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
tissue: Testis.
7.

"The transcriptional landscape of the mammalian genome."
Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
strain: C57BL/6J.
tissue: Embryonic kidney.
tissue: Liver tumor.
8.

"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M. , Goodstadt L. , Hillier L.W. , Zody M.C. , Goldstein S. , She X. , Bult C.J. , Agarwala R. , Cherry J.L. , DiCuccio M. , Hlavina W. , Kapustin Y. , Meric P. , Maglott D. , Birtle Z. , Marques A.C. , Graves T. , Zhou S. , more...
PLoS Biol.7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
strain: C57BL/6J.
9.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
strain: C57BL/6.
tissue: Eye.
10.

"Multiple functions of the TR2-11 orphan receptor in modulating activation of two key cis-acting elements involved in the retinoic acid signal transduction system."
Lin T.M. , Young W.J. , Chang C.
J. Biol. Chem.270:30121-30128(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DNA-BINDING;FUNCTION;TISSUE SPECIFICITY
11.

"The orphan nuclear receptor TR2 suppresses a DR4 hormone response element of the mouse CRABP-I gene promoter."
Chinpaisal C. , Chang L. , Hu X. , Lee C.-H. , Wen W.-N. , Wei L.-N.
Biochemistry36:14088-14095(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DNA-BINDING;HOMODIMERIZATION
12.

"Cloning and characterization of mouse RIP140, a corepressor for nuclear orphan receptor TR2."
Lee C.-H. , Chinpaisal C. , Wei L.-N.
Mol. Cell. Biol.18:6745-6755(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;INTERACTION WITH NRIP1
13.

"The orphan nuclear receptor TR2 interacts directly with both class I and class II histone deacetylases."
Franco P.J. , Farooqui M. , Seto E. , Wei L.-N.
Mol. Endocrinol.15:1318-1328(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH HDAC3 AND HDAC4
14.

"An embryonic/fetal beta-type globin gene repressor contains a nuclear receptor TR2/TR4 heterodimer."
Tanabe O. , Katsuoka F. , Campbell A.D. , Song W. , Yamamoto M. , Tanimoto K. , Engel J.D.
EMBO J.21:3434-3442(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY IN DRED COMPLEX;FUNCTION;SUBCELLULAR LOCATION;HETERODIMERIZATION;DEVELOPMENTAL STAGE
15.

"Spermatogenesis and testis development are normal in mice lacking testicular orphan nuclear receptor 2."
Shyr C.R. , Collins L.L. , Mu X.M. , Platt K.A. , Chang C.
Mol. Cell. Biol.22:4661-4666(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISRUPTION PHENOTYPE;TISSUE SPECIFICITY
16.

"Protein kinase C-mediated phosphorylation of orphan nuclear receptor TR2: effects on receptor stability and activity."
Khan S.A. , Park S.W. , Huq M. , Wei L.N.
Proteomics5:3885-3894(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-461 AND SER-568;FUNCTION;IDENTIFICATION BY MASS SPECTROMETRY
17.

"Ligand-independent orphan receptor TR2 activation by phosphorylation at the DNA-binding domain."
Khan S.A. , Park S.W. , Huq M.D. , Wei L.N.
Proteomics6:123-130(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-185;DNA-BINDING;FUNCTION;INTERACTION WITH KAT2B;MUTAGENESIS OF SER-170 AND SER-185
18.

"Embryonic and fetal beta-globin gene repression by the orphan nuclear receptors, TR2 and TR4."
Tanabe O. , McPhee D. , Kobayashi S. , Shen Y. , Brandt W. , Jiang X. , Campbell A.D. , Chen Y.T. , Chang C. , Yamamoto M. , Tanimoto K. , Engel J.D.
EMBO J.26:2295-2306(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;HETERODIMERIZATION
19.

"The TR2 and TR4 orphan nuclear receptors repress Gata1 transcription."
Tanabe O. , Shen Y. , Liu Q. , Campbell A.D. , Kuroha T. , Yamamoto M. , Engel J.D.
Genes Dev.21:2832-2844(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: HETERODIMERIZATION;FUNCTION
20.

"SUMOylation of Tr2 orphan receptor involves Pml and fine-tunes Oct4 expression in stem cells."
Park S.W. , Hu X. , Gupta P. , Lin Y.P. , Ha S.G. , Wei L.N.
Nat. Struct. Mol. Biol.14:68-75(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUMOYLATION AT LYS-238;SUBCELLULAR LOCATION;FUNCTION;INTERACTION WITH KAT2B; NRIP1; PIAS1 AND UBE2I;MUTAGENESIS OF LYS-167; LYS-238; GLU-240 AND LYS-575
21.

"Orphan nuclear receptor TR2, a mediator of preadipocyte proliferation, is differentially regulated by RA through exchange of coactivator PCAF with corepressor RIP140 on a platform molecule GRIP1."
Gupta P. , Park S.W. , Farooqui M. , Wei L.N.
Nucleic Acids Res.35:2269-2282(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
22.

"Retinoic acid-stimulated sequential phosphorylation, PML recruitment, and SUMOylation of nuclear receptor TR2 to suppress Oct4 expression."
Gupta P. , Ho P.C. , Huq M.M. , Ha S.G. , Park S.W. , Khan A.A. , Tsai N.P. , Wei L.N.
Proc. Natl. Acad. Sci. U.S.A.105:11424-11429(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-203; THR-208 AND THR-210;SUMOYLATION;INTERACTION WITH KAT2B;MUTAGENESIS OF SER-170; SER-185; SER-203; THR-208; THR-210; LYS-238; SER-461 AND SER-568;IDENTIFICATION BY MASS SPECTROMETRY
23.

"Roles of testicular orphan nuclear receptors 2 and 4 in early embryonic development and embryonic stem cells."
Shyr C.R. , Kang H.Y. , Tsai M.Y. , Liu N.C. , Ku P.Y. , Huang K.E. , Chang C.
Endocrinology150:2454-2462(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISRUPTION PHENOTYPE;INDUCTION;FUNCTION
24.

"HDAC3 as a molecular chaperone for shuttling phosphorylated TR2 to PML: a novel deacetylase activity-independent function of HDAC3."
Gupta P. , Ho P.C. , Ha S.G. , Lin Y.W. , Wei L.N.
PLoS ONE4:E4363-E4363(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUMOYLATION;INTERACTION WITH HDAC3;SUBCELLULAR LOCATION

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