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Index > Protein center > Otof(Gene name) > Rat
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  • Otof (Gene name),
  • Otoferlin (Protein name ),  OTOF_RAT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    Otof(Fer1l2);
    Protein name:
    Otoferlin;
    Alternative:
    Fer-1-like protein 2;
    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    Interacts with SNAP25; the interaction is direct. Interacts with STX1; the interaction is direct. Interacts with RAB8B.
    Function:
    Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion and in the control of neurotransmitter release at these output synapses. Interacts in a calcium-dependent manner to the presynaptic SNARE proteins at ribbon synapses of cochlear inner hair cells (IHCs) to trigger exocytosis of neurotransmitter. Also essential to synaptic exocytosis in immature outer hair cells (OHCs). May also play a role within the recycling of endosomes.
    Subcellular Location:
    Cytoplasmic vesicle secretory vesicle synaptic vesicle membrane Single-pass type II membrane protein Basolateral cell membrane Single-pass type II membrane protein Endoplasmic reticulum membrane Single-pass type II membrane protein Cell membrane Single-pass type II membrane protein Detected at basolateral cell membrane with synaptic vesicles surrounding the ribbon and at the presynaptic plasma membrane in the inner hair cells (IHCs). Colocalizes with GPR25 and RAB8B in inner hair cells.
    Protein Attributes:
    Sequence length:
    1993
    Sequence:
    50:
    MALIVHLKTV | SELRGRADRI | AKVTFRGQSF | YSRVLENCED | VADFDETFRW | 
    100:
    PVASSIDRNE | VLEIQIFNYS | KVFSNKLIGT | FRMVLQKVVE | ENRVEVSDTL | 
    150:
    IDDNNAIIKT | SLSMEVRYQA | ADGTVGPWDD | GDFLGDESLQ | EEEKDSQETD | 
    200:
    GLLPGSRPST | RTPGEKSFRS | KGKEKTKGGR | DGEHKAGRSV | FSAMKLGKTR | 
    250:
    SHKEEPQRQD | EPAVLEMEDL | DHLAIRLGDG | LDPDSVSLAS | VTALTSNVSN | 
    300:
    KRSKPDIKME | PSAGRPMDYQ | VSITVIEARQ | LVGLNMDPVV | CVEVGDDKKY | 
    350:
    TSMKESTNCP | YYNEYFVFDF | HVSPDVMFDK | IIKISVIHSK | NLLRSGTLVG | 
    400:
    SFKMDVGTVY | SQPEHQFHHK | WAILSDPDDI | SAGLKGYVKC | DVAVVGKGDN | 
    450:
    IKTPHKANET | DEDDIEGNLL | LPEGVPPERQ | WARFYVKIYR | AEGLPRMNTS | 
    500:
    LMANVKKAFI | GENKDLVDPY | VQVFFAGQKG | KTSVQKSSYE | PLWNEQVVFT | 
    550:
    DLFPPLCKRM | KVQIRDSDKV | NDVAIGTHFI | DLRKISNDGD | KGFLPTLGPA | 
    600:
    WVNMYGSTRN | YTLLDEHQDL | NEGLGEGVSF | RARLMLGLAV | EILDTSNPEL | 
    650:
    TSSTEVQVEQ | ATPVSESCTG | RMEEFFLFGA | FLEASMIDRK | NGDKPVTFEV | 
    700:
    TIGNYGNEVD | GTSRPQRPRP | RKEPGDEEEV | DLIQNSSDDE | GDEAGDLASV | 
    750:
    SSTPPMRPQI | TDRNYFHLPY | LERKPCIYIK | SWWPDQRRRL | YNANIMDHIA | 
    800:
    DKLEEGLNDV | QEMIKTEKSY | PERRLRGVLE | ELSCGCHRFL | SLSDKDQGHS | 
    850:
    SRTRLDRERL | KSCMRELESM | GQQAKSLRAQ | VKRHTVRDKL | RLCQNFLQKL | 
    900:
    RFLADEPQHS | IPDVFIWMMS | NNKRIAYARV | PSKDLLFSIV | EEELGKDCAK | 
    950:
    VKTLFLKLPG | KRGFGSAGWT | VQAKLELYLW | LGLSKQRKDF | LCGLPCGFEE | 
    1000:
    VKAAQGLGLH | SFPPISLVYT | KKQTFQLRAH | MYQARSLFAA | DSTGLSDPFA | 
    1050:
    RVFFINQSQC | TEVLNETLCP | TWDQMLVFDN | LELYGEAHEL | RDDPPIIVIE | 
    1100:
    IYDQDSMGKA | DFMGRTFAKP | LVKMADEAYC | PPRFPPQLEY | YQIYRGNATA | 
    1150:
    GDLLAAFELL | QIGPSGKADL | PPINGPVDMD | RGPIMPVPVG | IRPVLSKYRV | 
    1200:
    EVLFWGLRDL | KRVNLAQVDR | PRVDIECAGK | GVQSSLIHNY | KKNPNFNTLV | 
    1250:
    KWFEVDLPEN | ELLHPPLNIR | VVDCRAFGRY | TLVGSHAVSS | LRRFIYRPPD | 
    1300:
    RSAANWNTTG | EVVVSMEPEV | PVKKLETLVK | LDATSDAVVK | VDVAEDEKER | 
    1350:
    KKKKKKGPSE | EAEEEEPDES | MLDWWSKYFA | SIDTMKEQLR | QHETSGIDLE | 
    1400:
    EKEEMDSTEG | LKGPVKNKEK | SRAAKEEKKK | KNQNPGPGQG | SEAPEKKKAK | 
    1450:
    IDELKVYPKE | LESEFDNFED | WLHTFNLLRG | KTGDDEDGST | EEERIVGRFK | 
    1500:
    GSLCVYKVPL | PEDVSREAGY | DPTYGMFQGI | PSNDPINVLV | RIYVVRATDL | 
    1550:
    HPADINGKAD | PYIAIKLGKT | DIRDKENYIS | KQLNPVFGKS | FDIEASFPME | 
    1600:
    SMLTVAVYDW | DLVGTDDLIG | ETKIDLENRF | YSKHRATCGI | AQTYSIHGYN | 
    1650:
    IWRDPMKPSQ | ILTRLCKEGK | VDGPHFGPHG | RVKVANRVFT | GPSEIEDENG | 
    1700:
    QRKPTDEHVA | LSALRHWEDI | PRVGCRLVPE | HVETRPLLNP | DKPGIEQGRL | 
    1750:
    ELWVDMFPMD | MPAPGTPLDI | SPRKPKKYEL | RVIVWNTDEV | VLEDDDFFTG | 
    1800:
    EKSSDIFVRG | WLKGQQEDKQ | DTDVHYHSLT | GEGNFNWRYL | FPFDYLAAEE | 
    1850:
    KIVMSKKESM | FSWDETEYKI | PARLTLQIWD | ADHFSADDFL | GAIELDLNRF | 
    1900:
    PRGAKTAKQC | TMEMATGEVD | VPLVSIFKQK | RVKGWWPLLA | RNENDEFELT | 
    1950:
    GKVEAELHLL | TAEEAEKNPV | GLARNEPDPL | EKPNRPDTAF | VWFLNPLKSI | 
    1993:
    KYLICTRYKW | LIIKIVLALL | GLLMLALFLY | SLPGYMVKKL | LGA
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    KEGG:
    UniGene:
    String:
    Pfam:
    UniGene:
    Uniprot:
     
    FOR
    ELISA Kit for Rat Otoferlin
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    MSDS:
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    MSDS:
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    MSDS:
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    CLIA Kit for Rat Otoferlin
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    MSDS:
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    Packing:
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    CLIA Kit for Rat Otoferlin
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    U15538m
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Rat Otoferlin
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    MSDS:
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    Polyclonal Antibody for Rat Otoferlin
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    Polyclonal Antibody for Rat Otoferlin
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    Packing:
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    Monoclonal Antibody for Rat Otoferlin
    Monoclonal Antibody for Rat Otoferlin
    Monoclonal Antibody for Rat Otoferlin
    Protein for Rat Otoferlin
    Protein for Rat Otoferlin
    Protein for Rat Otoferlin

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    Linearity
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    References
    1. 1.
      "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
      Gibbs R.A. , Weinstock G.M. , Metzker M.L. , Muzny D.M. , Sodergren E.J. , Scherer S. , Scott G. , Steffen D. , Worley K.C. , Burch P.E. , Okwuonu G. , Hines S. , Lewis L. , Deramo C. , Delgado O. , Dugan-Rocha S. , Miner G. , Morgan M. , more...
      Nature428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
      strain: Brown Norway.
    2. 2.
      "Dynamic developmental expression of cochlear hair cell genes: prestin and otoferlin."
      Beisel K.W. , Nelson N.C. , Beisel C.L. , Delimont D.C. , He D.Z.Z. , Fritzsch B.
      Submitted (2000-10) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1554-1819
      strain: Sprague-Dawley.
    3. 3.
      "Differential expression of otoferlin in brain, vestibular system, immature and mature cochlea of the rat."
      Schug N. , Braig C. , Zimmermann U. , Engel J. , Winter H. , Ruth P. , Blin N. , Pfister M. , Kalbacher H. , Knipper M.
      Eur. J. Neurosci.24:3372-3380(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY
    4. 4.
      "Rab8b GTPase, a protein transport regulator, is an interacting partner of otoferlin, defective in a human autosomal recessive deafness form."
      Heidrych P. , Zimmermann U. , Bress A. , Pusch C.M. , Ruth P. , Pfister M. , Knipper M. , Blin N.
      Hum. Mol. Genet.17:3814-3821(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY
    5. 5.
      "The crystal structure of the CA domain of otoferlin reveals an unconventional top loop region."
      Helfmann S. , Neumann P. , Tittmann K. , Moser T. , Ficner R. , Reisinger E.
      J. Mol. Biol.406:479-490(2011) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 1-124;CIRCULAR DICHROISM;DOMAIN
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