Protein name:
Otoferlin ;
Alternative:
Fer-1-like protein 2 ;
Organism:
Rat (Rattus norvegicus).
General Annotation
Sub Unit:
Interacts with SNAP25; the interaction is direct. Interacts with STX1; the interaction is direct. Interacts with RAB8B.
Function:
Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion and in the control of neurotransmitter release at these output synapses. Interacts in a calcium-dependent manner to the presynaptic SNARE proteins at ribbon synapses of cochlear inner hair cells (IHCs) to trigger exocytosis of neurotransmitter. Also essential to synaptic exocytosis in immature outer hair cells (OHCs). May also play a role within the recycling of endosomes.
Subcellular Location:
Cytoplasmic vesicle
secretory vesicle
synaptic vesicle membrane
Single-pass type II membrane protein
Basolateral cell membrane
Single-pass type II membrane protein
Endoplasmic reticulum membrane
Single-pass type II membrane protein
Cell membrane
Single-pass type II membrane protein
Detected at basolateral cell membrane with synaptic vesicles surrounding the ribbon and at the presynaptic plasma membrane in the inner hair cells (IHCs). Colocalizes with GPR25 and RAB8B in inner hair cells.
Protein Attributes:
50:
MALIVHLKTV | SELRGRADRI | AKVTFRGQSF | YSRVLENCED | VADFDETFRW |
100:
PVASSIDRNE | VLEIQIFNYS | KVFSNKLIGT | FRMVLQKVVE | ENRVEVSDTL |
150:
IDDNNAIIKT | SLSMEVRYQA | ADGTVGPWDD | GDFLGDESLQ | EEEKDSQETD |
200:
GLLPGSRPST | RTPGEKSFRS | KGKEKTKGGR | DGEHKAGRSV | FSAMKLGKTR |
250:
SHKEEPQRQD | EPAVLEMEDL | DHLAIRLGDG | LDPDSVSLAS | VTALTSNVSN |
300:
KRSKPDIKME | PSAGRPMDYQ | VSITVIEARQ | LVGLNMDPVV | CVEVGDDKKY |
350:
TSMKESTNCP | YYNEYFVFDF | HVSPDVMFDK | IIKISVIHSK | NLLRSGTLVG |
400:
SFKMDVGTVY | SQPEHQFHHK | WAILSDPDDI | SAGLKGYVKC | DVAVVGKGDN |
450:
IKTPHKANET | DEDDIEGNLL | LPEGVPPERQ | WARFYVKIYR | AEGLPRMNTS |
500:
LMANVKKAFI | GENKDLVDPY | VQVFFAGQKG | KTSVQKSSYE | PLWNEQVVFT |
550:
DLFPPLCKRM | KVQIRDSDKV | NDVAIGTHFI | DLRKISNDGD | KGFLPTLGPA |
600:
WVNMYGSTRN | YTLLDEHQDL | NEGLGEGVSF | RARLMLGLAV | EILDTSNPEL |
650:
TSSTEVQVEQ | ATPVSESCTG | RMEEFFLFGA | FLEASMIDRK | NGDKPVTFEV |
700:
TIGNYGNEVD | GTSRPQRPRP | RKEPGDEEEV | DLIQNSSDDE | GDEAGDLASV |
750:
SSTPPMRPQI | TDRNYFHLPY | LERKPCIYIK | SWWPDQRRRL | YNANIMDHIA |
800:
DKLEEGLNDV | QEMIKTEKSY | PERRLRGVLE | ELSCGCHRFL | SLSDKDQGHS |
850:
SRTRLDRERL | KSCMRELESM | GQQAKSLRAQ | VKRHTVRDKL | RLCQNFLQKL |
900:
RFLADEPQHS | IPDVFIWMMS | NNKRIAYARV | PSKDLLFSIV | EEELGKDCAK |
950:
VKTLFLKLPG | KRGFGSAGWT | VQAKLELYLW | LGLSKQRKDF | LCGLPCGFEE |
1000:
VKAAQGLGLH | SFPPISLVYT | KKQTFQLRAH | MYQARSLFAA | DSTGLSDPFA |
1050:
RVFFINQSQC | TEVLNETLCP | TWDQMLVFDN | LELYGEAHEL | RDDPPIIVIE |
1100:
IYDQDSMGKA | DFMGRTFAKP | LVKMADEAYC | PPRFPPQLEY | YQIYRGNATA |
1150:
GDLLAAFELL | QIGPSGKADL | PPINGPVDMD | RGPIMPVPVG | IRPVLSKYRV |
1200:
EVLFWGLRDL | KRVNLAQVDR | PRVDIECAGK | GVQSSLIHNY | KKNPNFNTLV |
1250:
KWFEVDLPEN | ELLHPPLNIR | VVDCRAFGRY | TLVGSHAVSS | LRRFIYRPPD |
1300:
RSAANWNTTG | EVVVSMEPEV | PVKKLETLVK | LDATSDAVVK | VDVAEDEKER |
1350:
KKKKKKGPSE | EAEEEEPDES | MLDWWSKYFA | SIDTMKEQLR | QHETSGIDLE |
1400:
EKEEMDSTEG | LKGPVKNKEK | SRAAKEEKKK | KNQNPGPGQG | SEAPEKKKAK |
1450:
IDELKVYPKE | LESEFDNFED | WLHTFNLLRG | KTGDDEDGST | EEERIVGRFK |
1500:
GSLCVYKVPL | PEDVSREAGY | DPTYGMFQGI | PSNDPINVLV | RIYVVRATDL |
1550:
HPADINGKAD | PYIAIKLGKT | DIRDKENYIS | KQLNPVFGKS | FDIEASFPME |
1600:
SMLTVAVYDW | DLVGTDDLIG | ETKIDLENRF | YSKHRATCGI | AQTYSIHGYN |
1650:
IWRDPMKPSQ | ILTRLCKEGK | VDGPHFGPHG | RVKVANRVFT | GPSEIEDENG |
1700:
QRKPTDEHVA | LSALRHWEDI | PRVGCRLVPE | HVETRPLLNP | DKPGIEQGRL |
1750:
ELWVDMFPMD | MPAPGTPLDI | SPRKPKKYEL | RVIVWNTDEV | VLEDDDFFTG |
1800:
EKSSDIFVRG | WLKGQQEDKQ | DTDVHYHSLT | GEGNFNWRYL | FPFDYLAAEE |
1850:
KIVMSKKESM | FSWDETEYKI | PARLTLQIWD | ADHFSADDFL | GAIELDLNRF |
1900:
PRGAKTAKQC | TMEMATGEVD | VPLVSIFKQK | RVKGWWPLLA | RNENDEFELT |
1950:
GKVEAELHLL | TAEEAEKNPV | GLARNEPDPL | EKPNRPDTAF | VWFLNPLKSI |
1993:
KYLICTRYKW | LIIKIVLALL | GLLMLALFLY | SLPGYMVKKL | LGA
Vaild Sequence:
Related Databases
Uniprot:
ELISA Kit
CLIA Kit
Polyclonal Antibody
Monoclonal Antibody
Protein
FOR
Rat
Mouse
Human
ELISA Kit for Rat Otoferlin
ELISA Kit for Rat Otoferlin
ELISA Kit for Rat Otoferlin
CLIA Kit for Rat Otoferlin
CLIA Kit for Rat Otoferlin
CLIA Kit for Rat Otoferlin
Polyclonal Antibody for Rat Otoferlin
Polyclonal Antibody for Rat Otoferlin
Polyclonal Antibody for Rat Otoferlin
Monoclonal Antibody for Rat Otoferlin
Monoclonal Antibody for Rat Otoferlin
Monoclonal Antibody for Rat Otoferlin
Protein for Rat Otoferlin
Protein for Rat Otoferlin
Protein for Rat Otoferlin
R&D Technical Data
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Precision
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Recovery
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Linearity
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more...
Nature428:493-521(2004)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
strain :
Brown Norway .
2.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [MRNA] OF 1554-1819
strain :
Sprague-Dawley .
3.
"Differential expression of otoferlin in brain, vestibular system, immature and mature cochlea of the rat."
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Eur. J. Neurosci.24:3372-3380(2006)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : TISSUE SPECIFICITY
4.
"Rab8b GTPase, a protein transport regulator, is an interacting partner of otoferlin, defective in a human autosomal recessive deafness form."
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[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : TISSUE SPECIFICITY
5.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 1-124;CIRCULAR DICHROISM;DOMAIN