PRNP ELISA Kit for Bovine(Bos taurus) | EIAab.comPRNP ELISA Kit for Bovine(Bos taurus)

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Index > Protein center > PRNP(Gene name) > Bovine

PRNP (Gene name),

Major prion protein (Protein name ), PRIO_BOVIN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

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Gene name:

PRNP(PRP);

Protein name:

Major prion protein(PrP);

Alternative:

Major scrapie-associated fibril protein 1;

CD:

CD230;

Organism:

Bovine (Bos taurus).

General Annotation

Sub Unit:

Monomer and homodimer. Has a tendency to aggregate into amyloid fibrils containing a cross-beta spine, formed by a steric zipper of superposed beta-strands. Soluble oligomers may represent an intermediate stage on the path to fibril formation. Copper binding may promote oligomerization. Interacts with APP, GRB2, ERI3/PRNPIP and SYN1. Mislocalized cytosolically exposed PrP interacts with MGRN1; this interaction alters MGRN1 subcellular location and causes lysosomal enlargement.

Function:

The function of PrP is still under debate. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro).

Subcellular Location:

Cell membrane Lipid-anchor GPI-anchor Golgi apparatus

Protein Attributes:

Sequence length:

264

Sequence:

50:

100:

150:

200:

250:

264:

VILLISFLIF | LIVG

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Bt.4737

String:

P10279

Pfam:

PF00377

SMR:

P10279

KEGG:

bta:281427

Uniprot:

P10279

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ELISA Kit for Bovine PrP

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E9345h

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96T

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ELISA Kit for Bovine PrP

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E9345m

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96T

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ELISA Kit for Bovine PrP

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E9345c

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ELISA Kit for Bovine PrP

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ELISA Kit for Bovine PrP

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E9345r

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ELISA Kit for Bovine PrP

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E9345b

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ELISA Kit for Bovine PrP

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ELISA Kit for Bovine PrP

Cat.:

E9345p

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96T

CLIA Kit for Bovine PrP

Cat.:

U9345h

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96T

CLIA Kit for Bovine PrP

Cat.:

U9345r

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96T

CLIA Kit for Bovine PrP

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U9345m

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96T

CLIA Kit for Bovine PrP

Cat.:

U9345b

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96T

CLIA Kit for Bovine PrP

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CLIA Kit for Bovine PrP

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CLIA Kit for Bovine PrP

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CLIA Kit for Bovine PrP

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U9345p

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96T

Polyclonal Antibody for Bovine PrP

Cat.:

P9345Rb-h

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40ug/0.2ml

Polyclonal Antibody for Bovine PrP

Cat.:

P9345Rb-m

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40ug/0.2ml

Monoclonal Antibody for Bovine PrP

Protein for Bovine PrP

R&D Technical Data

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.6%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-106
Plasma	98	92-108

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-102%	92-103%	95-105%	92-107%
EDTA plasma(n=5)	88-96%	93-101%	94-102%	95-106%
heparin plasma(n=5)	93-104%	94-105%	101-106%	95-109%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Different forms of the bovine PrP gene have five or six copies of a short, G-C-rich element within the protein-coding exon."
Goldmann W. , Hunter N. , Martin T. , Dawson M. , Hope J.
J. Gen. Virol.72:201-204(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
strain: Holstein-Friesian.
2.

"Comparative sequence analysis and expression of bovine PrP gene in mouse L-929 cells."
Yoshimoto J. , Iinuma T. , Ishiguro N. , Horiuchi M. , Imamura M. , Shinagawa M.
Virus Genes6:343-356(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA];VARIANTS 92-TRP--GLY 99 DEL AND ASN-154
strain: Holstein-Friesian.
tissue: Brain.
3.

"Immunologic and molecular biologic studies of prion proteins in bovine spongiform encephalopathy."
Prusiner S.B. , Fuzi M. , Scott M. , Serban D. , Serban H. , Taraboulos A. , Gabriel J.M. , Wells G.A. , Wilesmith J.W. , Bradley R.
J. Infect. Dis.167:602-613(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
4.

"Genomic organization of bovine PrP gene and complete nucleotide sequence of bovine PrP cDNA."
Horiuchi M. , Ishiguro N. , Nagasawa H. , Toyoda Y. , Shinagawa M.
Submitted (1997-02) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Holstein-Friesian.
tissue: Brain.
5.

"Complete genomic sequence of the bovine prion gene (PRNP) and polymorphism in its promoter region."
Hills D. , Comincini S. , Schlaepfer J. , Dolf G. , Ferretti L. , Williams J.L.
Anim. Genet.32:231-232(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
strain: Jersey.
6.

"Bovine PrP gene for prion protein."
Naharro G. , Yugueros J. , Temprano A.
Submitted (2001-12) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT 98-GLY-GLY-TRP-GLY-GLN-PRO-HIS-GLY-GLY INS
7.

"Nucleotide sequence of PrP cDNA in Korean cattle."
Yoo H.S. , Kang S.G. , Choi I.S. , Kang S.K. , Hwang W.S.
Submitted (2002-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Korean.
8.

"Prion gene sequence variation within diverse groups of U.S. sheep, beef cattle, and deer."
Heaton M.P. , Leymaster K.A. , Freking B.A. , Hawk D.A. , Smith T.P. , Keele J.W. , Snelling W.M. , Fox J.M. , Chitko-McKown C.G. , Laegreid W.W.
Mamm. Genome14:765-777(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
9.

"Prevalence of the prion protein gene E211K variant in U.S. cattle."
Heaton M.P. , Keele J.W. , Harhay G.P. , Richt J.A. , Koohmaraie M. , Wheeler T.L. , Shackelford S.D. , Casas E. , King D.A. , Sonstegard T.S. , Van Tassell C.P. , Neibergs H.L. , Chase C.C. Jr. , Kalbfleisch T.S. , Smith T.P.L. , Clawson M.L. , Laegreid W.W.
BMC Vet. Res.4:25-25(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SEQUENCE REVISION TO 211;VARIANT LYS-211
10.

"Cloning and sequence analysis of bovine prion protein gene."
Wu R. , Xie Q.G. , Liu X.T. , Chen H.T. , Cheng J.
Submitted (2003-08) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS ARG-60; ARG-94; ASN-154 AND ARG-234
strain: Holstein-Friesian.
strain: Qinchuan.
tissue: Blood.
11.

"Prion protein gene (PRNP) variants and evidence for strong purifying selection in functionally important regions of bovine exon 3."
Seabury C.M. , Honeycutt R.L. , Rooney A.P. , Halbert N.D. , Derr J.N.
Proc. Natl. Acad. Sci. U.S.A.101:15142-15147(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS 84-TRP--GLY 99 DEL; 92-TRP--GLY 99 DEL AND 98-GLY-GLY-TRP-GLY-GLN-PRO-HIS-GLY-GLY INS
12.

"Genomic characterization of the bovine PRN loci (PRNP, PRND and PRNT)."
Uboldi C. , Bertoni A. , Del Vecchio I. , Comincini S. , Hills D. , Schlaepfer J. , Dolf G. , Williams J.L. , Ferretti L.
Submitted (2005-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
13.

"Comparative genomic organization of the human and bovine PRNP locus."
Choi S.-H. , Kim I.-C. , Kim D.-S. , Kim D.-W. , Chae S.-H. , Choi H.-H. , Choi I. , Yeo J.-S. , Song M.-N. , Park H.-S.
Genomics87:598-607(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
14.

"Frequencies of bovine PrP gene polymorphisms in Japanese-black and Holstein bulls in japan."
Abe T. , Hasebe H. , Kobayashi E.
Submitted (2006-01) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
strain: Holstein.
strain: Japanese black.
tissue: Semen.
15.

"Detection of prion protein in various animal species by a new set of monoclonal antibodies."
Barbieri I. , Brocchi E. , Borre A. , Moretti M. , Gelmetti D. , Capucci L.
Submitted (2006-11) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
tissue: Brain.
16.

NIH - Mammalian Gene Collection (MGC) project
Submitted (2006-08) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain: Hereford.
tissue: Hypothalamus.
17.

"Characterization of bovine PrP promoter region."
Tanaka M. , Inoue S. , Ikeda T. , Horiuchi M. , Ishiguro N. , Shinagawa M.
Submitted (1994-01) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-15
18.

"Fibrils from brains of cows with new cattle disease contain scrapie-associated protein."
Hope J. , Reekie L.J.D. , Hunter N. , Multhaup G. , Beyreuther K. , White H. , Scott A.C. , Stack M.J. , Dawson M. , Wells G.A.
Nature336:390-392(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 25-36
19.

"NMR solution structure and membrane interaction of the N-terminal sequence (1-30) of the bovine prion protein."
Biverstahl H. , Andersson A. , Graslund A. , Maler L.
Biochemistry43:14940-14947(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 1-30
20.

"NMR structure of the bovine prion protein."
Lopez Garcia F. , Zahn R. , Riek R. , Wuethrich K.
Proc. Natl. Acad. Sci. U.S.A.97:8334-8339(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 132-241
21.

"Directed evolution of an anti-prion protein scFv fragment to an affinity of 1 pM and its structural interpretation."
Luginbuhl B. , Kanyo Z. , Jones R.M. , Fletterick R.J. , Prusiner S.B. , Cohen F.E. , Williamson R.A. , Burton D.R. , Pluckthun A.
J. Mol. Biol.363:75-97(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) OF 107-115 IN COMPLEX WITH ANTIBODY FRAGMENT

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