PFN1 ELISA Kit for Human(Homo sapiens) | EIAab.comPFN1 ELISA Kit for Human(Homo sapiens) | EIAab.com

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PFN1 (Gene name),

Profilin-1 (Protein name ), PROF1_HUMAN from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Citations (1)

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Gene name:

PFN1;

Protein name:

Profilin-1;

Alternative:

Profilin I;Profilin I;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio. Found in a complex with XPO6, Ran, ACTB and PFN1. Interacts with VASP. Interacts with HTT.

Function:

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.

Subcellular Location:

Cytoplasm cytoskeleton

Protein Attributes:

Sequence length:

140

Sequence:

50:

MAGWNAYIDN | LMADGTCQDA | AIVGYKDSPS | VWAAVPGKTF | VNITPAEVGV |

100:

LVGKDRSSFY | VNGLTLGGQK | CSVIRDSLLQ | DGEFSMDLRT | KSTGGAPTFN |

140:

VTVTKTDKTL | VLLMGKEGVH | GGLINKKCYE | MASHLRRSQY |

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

MIM:

Pfam:

UniGene:

SMR:

STRING:

9606.ENSP00000225655

KEGG:

Uniprot:

FOR

ELISA Kit for Human Profilin-1

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E2122h

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96T

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ELISA Kit for Human Profilin-1

Cat.:

E2122b

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MSDS:

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Packing:

96T

ELISA Kit for Human Profilin-1

Cat.:

E2122r

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96T

ELISA Kit for Human Profilin-1

Cat.:

E2122m

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96T

CLIA Kit for Human Profilin-1

Cat.:

U2122b

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96T

CLIA Kit for Human Profilin-1

Cat.:

U2122h

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96T

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CLIA Kit for Human Profilin-1

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U2122m

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96T

CLIA Kit for Human Profilin-1

Cat.:

U2122r

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96T

Polyclonal Antibody for Human Profilin-1

Cat.:

P2122Rb-h

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40ug/0.2ml

Polyclonal Antibody for Human Profilin-1

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P2122Rb-m

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Polyclonal Antibody for Human Profilin-1

Polyclonal Antibody for Human Profilin-1

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P2122Rb-r

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40ug/0.2ml

Monoclonal Antibody for Human Profilin-1

Monoclonal Antibody for Human Profilin-1

Monoclonal Antibody for Human Profilin-1

Monoclonal Antibody for Human Profilin-1

Protein for Human Profilin-1

Protein for Human Profilin-1

Protein for Human Profilin-1

Protein for Human Profilin-1

R&D Technical Data

s

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s

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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.1%

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

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Recovery

Recovery was determined by spiking various levels of PROF1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	100	93-105
Plasma	101	95-106

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PROF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	94-106%	96-103%	94-105%	95-107%
EDTA plasma(n=5)	95-107%	96-105%	95-107%	94-106%
heparin plasma(n=5)	97-104%	93-108%	92-109%	97-105%

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

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Citations

[1].

"Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical Implementation"

Chatziharalambous D, Lygirou V, Latosinska A, et al

References

1.

"Human profilin. Molecular cloning, sequence comparison, and chromosomal analysis."
Kwiatkowski D.J. , Bruns G.A.P.
J. Biol. Chem.263:5910-5915(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
2.

"Systematic mapping and functional analysis of a family of human epididymal secretory sperm-located proteins."
Li J. , Liu F. , Wang H. , Liu X. , Liu J. , Li N. , Wan F. , Wang W. , Zhang C. , Jin S. , Liu J. , Zhu P. , Liu Y.
Mol. Cell. Proteomics9:2517-2528(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];TISSUE SPECIFICITY
tissue: Epididymis.
3.

"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
Submitted (2003-05) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
4.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Brain.
5.

"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L. , Schick M. , Neubert P. , Schatten R. , Henze S. , Korn B.
Submitted (2004-05) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
6.

Mural R.J. , Istrail S. , Sutton G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
7.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Colon.
tissue: Lung.
tissue: Pancreas.
tissue: Placenta.
8.

"The primary structure of human platelet profilin: reinvestigation of the calf spleen profilin sequence."
Ampe C. , Markey F. , Lindberg U. , Vandekerckhove J.
FEBS Lett.228:17-21(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-140;ACETYLATION AT ALA-2
9.

"Distinct biochemical characteristics of the two human profilin isoforms."
Gieselmann R. , Kwiatkowski D.J. , Janmey P.A. , Witke W.
Eur. J. Biochem.229:621-628(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION
10.

"Exportin 6: a novel nuclear export receptor that is specific for profilin.actin complexes."
Stueven T. , Hartmann E. , Goerlich D.
EMBO J.22:5928-5940(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION IN A COMPLEX WITH RAN; ACTB AND XPO6
11.

"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Rush J. , Moritz A. , Lee K.A. , Guo A. , Goss V.L. , Spek E.J. , Zhang H. , Zha X.-M. , Polakiewicz R.D. , Comb M.J.
Nat. Biotechnol.23:94-101(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-129;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
12.

"Phosphorylation of profilin by ROCK1 regulates polyglutamine aggregation."
Shao J. , Welch W.J. , Diprospero N.A. , Diamond M.I.
Mol. Cell. Biol.28:5196-5208(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH HTT;PHOSPHORYLATION AT SER-138
13.

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
14.

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Leukemic T-cell.
15.

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C. , Kumar C. , Gnad F. , Nielsen M.L. , Rehman M. , Walther T.C. , Olsen J.V. , Mann M.
Science325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-105 AND LYS-108;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
16.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
17.

"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V. , Sumpton D. , Martinez A. , Lilla S. , Espagne C. , Meinnel T. , Giglione C.
Mol. Cell. Proteomics11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
18.

"Characterization of the three-dimensional solution structure of human profilin: 1H, 13C, and 15N NMR assignments and global folding pattern."
Metzler W.J. , Constantine K.L. , Friedrichs M.S. , Bell A.J. , Ernst E.G. , Lavoie T.B. , Mueller L.
Biochemistry32:13818-13829(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR
19.

Fedorov A.A. , Pollard T.D. , Almo S.C.
Submitted (1996-04) to the PDB data bank

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS)
20.

"Structure of the profilin-poly-L-proline complex involved in morphogenesis and cytoskeletal regulation."
Mahoney N.M. , Janmey P.A. , Almo S.C.
Nat. Struct. Biol.4:953-960(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF COMPLEX WITH POLY-PRO
21.

"Profilin binds proline-rich ligands in two distinct amide backbone orientations."
Mahoney N.M. , Rozwarski D.A. , Fedorov E. , Fedorov A.A. , Almo S.C.
Nat. Struct. Biol.6:666-671(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF COMPLEX WITH POLY-PRO
22.

"Structural basis for the recruitment of profilin-actin complexes during filament elongation by Ena/VASP."
Ferron F. , Rebowski G. , Lee S.H. , Dominguez R.
EMBO J.26:4597-4606(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 2-140 IN COMPLEXES WITH VASP AND MONOMERIC ACTIN;INTERACTION WITH VASP
23.

"Modulation of actin structure and function by phosphorylation of Tyr-53 and profilin binding."
Baek K. , Liu X. , Ferron F. , Shu S. , Korn E.D. , Dominguez R.
Proc. Natl. Acad. Sci. U.S.A.105:11748-11753(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 2-140 IN COMPLEX WITH VASP AND ACTIN
24.

"Mutations in the profilin 1 gene cause familial amyotrophic lateral sclerosis."
Wu C.H. , Fallini C. , Ticozzi N. , Keagle P.J. , Sapp P.C. , Piotrowska K. , Lowe P. , Koppers M. , McKenna-Yasek D. , Baron D.M. , Kost J.E. , Gonzalez-Perez P. , Fox A.D. , Adams J. , Taroni F. , Tiloca C. , Leclerc A.L. , Chafe S.C. , more...
Nature488:499-503(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS ALS18 GLY-71; THR-114; GLY-117 AND VAL-118;CHARACTERIZATION OF VARIANTS ALS18 GLY-71; THR-114; GLY-117 AND VAL-118

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