Runx1 Polyclonal Antibody for Mouse(Mus musculus) | EIAab.comRunx1 Polyclonal Antibody for Mouse(Mus musculus)

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Runx1 (Gene name),

Runt-related transcription factor 1 (Protein name ), RUNX1_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Runx1(Aml1;Cbfa2;Pebp2ab);

Protein name:

Runt-related transcription factor 1;

Alternative:

Core-binding factor subunit alpha-2(CBF-alpha-2);Acute myeloid leukemia 1 protein;Polyomavirus enhancer-binding protein 2 alpha B subunit(PEA2-alpha B;PEBP2-alpha B);Oncogene AML-1;SL3/AKV core-binding factor alpha B subunit;SL3-3 enhancer factor 1 alpha B subunit;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Heterodimer with CBFB. RUNX1 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with TLE1 and THOC4. Interacts with HIPK2, ELF1, ELF2 and SPI1. Interacts via its Runt domain with the ELF4 N-terminal region. Interaction with ELF2 isoform 2 (NERF-1a) may act to repress RUNX1-mediated transactivation. Interacts with KAT6A and KAT6B. Interacts with SUV39H1, leading to abrogation of transactivating and DNA-binding properties of RUNX1 (By similarity). Interacts with YAP1. Interaction with CDK6 prevents myeloid differentiation, reducing its transcription transactivation activity.

Function:

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. Essential for the development of normal hematopoiesis. Isoform 4 shows higher binding activities for target genes and binds TCR-beta-E2 and RAG-1 target site with threefold higher affinity than other isoforms. It is less effective in the context of neutrophil terminal differentiation. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter. Inhibits KAT6B-dependent transcriptional activation.

Subcellular Location:

Nucleus

Protein Attributes:

Sequence length:

451

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

451:

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

SMR:

Q03347

String:

Q03347

Pfam:

PF00853

UniGene:

Mm.4081

KEGG:

mmu:12394

Uniprot:

Q03347

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ELISA Kit for Mouse Runt-related transcription factor 1

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E10487h

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96T

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ELISA Kit for Mouse Runt-related transcription factor 1

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E10487m

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ELISA Kit for Mouse Runt-related transcription factor 1

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E10487r

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96T

CLIA Kit for Mouse Runt-related transcription factor 1

Cat.:

U10487m

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96T

CLIA Kit for Mouse Runt-related transcription factor 1

Cat.:

U10487h

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MSDS:

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Packing:

96T

CLIA Kit for Mouse Runt-related transcription factor 1

Cat.:

U10487r

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96T

Polyclonal Antibody for Mouse Runt-related transcription factor 1

Cat.:

P10487Rb-h

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40ug/0.2ml

Polyclonal Antibody for Mouse Runt-related transcription factor 1

Cat.:

P10487Rb-r

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40ug/0.2ml

Polyclonal Antibody for Mouse Runt-related transcription factor 1

Cat.:

P10487Rb-m

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Packing:

40ug/0.2ml

Monoclonal Antibody for Mouse Runt-related transcription factor 1

Protein for Mouse Runt-related transcription factor 1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Isolation of PEBP2 alpha B cDNA representing the mouse homolog of human acute myeloid leukemia gene, AML1."
Bae S.-C. , Yamaguchi-Iwai Y. , Ogawa E. , Maruyama M. , Inuzuka M. , Kagoshima H. , Shigesada K. , Satake M. , Ito Y.
Oncogene8:809-814(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
tissue: Fibroblast.
2.

"PEBP2 alpha B/mouse AML1 consists of multiple isoforms that possess differential transactivation potentials."
Bae S.-C. , Ogawa E. , Maruyama M. , Oka H. , Satake M. , Shigesada K. , Jenkins N.A. , Gilbert D.J. , Copeland N.G. , Ito Y.
Mol. Cell. Biol.14:3242-3252(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)
tissue: Fibroblast.
3.

Calabi F.
Submitted (1996-04) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3)
strain: BALB/c.
tissue: Thymus.
4.

"Identification and expression of a novel 3'-exon of mouse Runx1/Pebp2alphaB/Cbfa2/AML1 gene."
Tsuji K. , Noda M.
Biochem. Biophys. Res. Commun.274:171-176(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5)
5.

"Identification of an alternatively spliced form of the mouse AML1/RUNX1 gene transcript AML1c and its expression in early hematopoietic development."
Fujita Y. , Nishimura M. , Taniwaki M. , Abe T. , Okuda T.
Biochem. Biophys. Res. Commun.281:1248-1255(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4)
strain: 129/Ola.
tissue: Embryo.
6.

"Expression and function of a stem cell promoter for the murine CBFalpha2 gene: distinct roles and regulation in natural killer and T cell development."
Telfer J.C. , Rothenberg E.V.
Dev. Biol.229:363-382(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA];ALTERNATIVE SPLICING (ISOFORM 4)
strain: C57BL/6.
tissue: Thymus.
7.

"AML1, the target of multiple chromosomal translocations in human leukemia, is essential for normal fetal liver hematopoiesis."
Okuda T. , van Deursen J. , Hiebert S.W. , Grosveld G. , Downing J.R.
Cell84:321-330(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN LIVER HEMATOPOIESIS
8.

"The histone- and PRMT5-associated protein COPR5 is required for myogenic differentiation."
Paul C. , Sardet C. , Fabbrizio E.
Cell Death Differ.19:900-908(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION IN A COMPLEX WITH PRMT5; RUNX1 AND CBFB
9.

"Disruption of the Cbfa2 gene causes necrosis and hemorrhaging in the central nervous system and blocks definitive hematopoiesis."
Wang Q. , Stacy T. , Binder M. , Marin-Padilla M. , Sharpe A.H. , Speck N.A.
Proc. Natl. Acad. Sci. U.S.A.93:3444-3449(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN NECROSIS AND HEMORRHAGING
10.

"Roles of HIPK1 and HIPK2 in AML1- and p300-dependent transcription, hematopoiesis and blood vessel formation."
Aikawa Y. , Nguyen L.A. , Isono K. , Takakura N. , Tagata Y. , Schmitz M.L. , Koseki H. , Kitabayashi I.
EMBO J.25:3955-3965(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HIPK2;PHOSPHORYLATION AT SER-249 AND SER-276 BY HIPK2;MUTAGENESIS OF SER-249 AND SER-276
11.

"Crystallization and preliminary X-ray analyses of quaternary, ternary and binary protein-DNA complexes with involvement of AML1/Runx-1/CBFalpha Runt domain, CBFbeta and the C/EBPbeta bZip region."
Tahirov T.H. , Inoue-Bungo T. , Sasaki M. , Shiina M. , Kimura K. , Sato K. , Kumasaka T. , Yamamoto M. , Kamiya N. , Ogata K.
Acta Crystallogr. D57:850-853(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) OF 62-177 IN COMPLEX WITH CEBPB; CBFB AND DNA
12.

"Structural analyses of DNA recognition by the AML1/Runx-1 Runt domain and its allosteric control by CBFbeta."
Tahirov T.H. , Inoue-Bungo T. , Morii H. , Fujikawa A. , Sasaki M. , Kimura K. , Shiina M. , Sato K. , Kumasaka T. , Yamamoto M. , Ishii S. , Ogata K.
Cell104:755-767(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) OF 60-182 IN COMPLEX WITH CEBPB; CBFB AND DNA;MUTAGENESIS OF ARG-80; ASN-109; TYR-113; ARG-142; LYS-144; THR-149; VAL-170; ASP-171; ARG-174 AND ARG-177
13.

"The RUNX1 Runt domain at 1.25A resolution: a structural switch and specifically bound chloride ions modulate DNA binding."
Baeckstroem S. , Wolf-Watz M. , Grundstroem C. , Haerd T. , Grundstroem T. , Sauer U.H.
J. Mol. Biol.322:259-272(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.25 ANGSTROMS) OF 60-173

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