RARB ELISA Kit for Chicken(Gallus gallus) | EIAab.comRARB ELISA Kit for Chicken(Gallus gallus) | EIAab.com

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RARB (Gene name),

Retinoic acid receptor beta (Protein name ), RARB_CHICK from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

RARB(NR1B2);

Protein name:

Retinoic acid receptor beta(RAR-beta);

Alternative:

Nuclear receptor subfamily 1 group B member 2;

Organism:

Chicken (Gallus gallus).

General Annotation

Sub Unit:

Heterodimer; with a RXR molecule (By similarity). Binds DNA preferentially as a RAR/RXR heterodimer.

Function:

Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5 (By similarity). Required for limb and craniofacial development.

Subcellular Location:

Nucleus

Protein Attributes:

Sequence length:

455

Sequence:

50:

MTTSSRTCPV | PAVNGHMTHY | PAAPYPLLFP | PVIGGLSLPS | LHGLQSHPPT |

100:

SGCSTPSPAT | VETQSTSSEE | LVPSPPSPLP | PPRVYKPCFV | CQDKSSGYHY |

150:

GVSACEGCKG | FFRRSIQKNM | VYTCHRDKNC | VINKVTRNRC | QYCRLQKCFE |

200:

VGMSKESVRN | DRNKKKKEPT | KQESTENYEM | TAELDDLTEK | IRKAHQETFP |

250:

SLCQLGKYTT | NSSADHRVRL | DLGLWDKFSE | LATKCIIKIV | EFAKRLPGFT |

300:

SLTIADQITL | LKAACLDILI | LRICTRYTPE | QDTMTFSDGL | TLNRTQMHNA |

350:

GFGPLTDLVF | TFANQLLPLE | MDDTETGLLS | AICLICGDRQ | DLEEPMKVDK |

400:

LQEPLLEALK | IYIRKRRPNK | PHMFPKILMK | ITDLRSISAK | GAERVITLKM |

450:

EIPGSMPPLI | QEMLENSEGH | EPLTPTSNGN | TAEHSPSISP | SSVDNSSVSQ |

455:

SPMVQ

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

SMR:

Pfam:

String:

KEGG:

UniGene:

Uniprot:

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ELISA Kit for Chicken RAR-beta

Cat.:

E10642m

Price:

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Manual:

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MSDS:

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Packing:

96T

Range:

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ELISA Kit for Chicken RAR-beta

Cat.:

E10642h

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Manual:

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Packing:

96T

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ELISA Kit for Chicken RAR-beta

MSDS:

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CLIA Kit for Chicken RAR-beta

MSDS:

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CLIA Kit for Chicken RAR-beta

Cat.:

U10642h

Price:

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Manual:

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MSDS:

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Packing:

96T

Range:

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CLIA Kit for Chicken RAR-beta

Cat.:

U10642m

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Polyclonal Antibody for Chicken RAR-beta

Polyclonal Antibody for Chicken RAR-beta

Polyclonal Antibody for Chicken RAR-beta

Cat.:

P10642Rb-h

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Chicken RAR-beta

Monoclonal Antibody for Chicken RAR-beta

Monoclonal Antibody for Chicken RAR-beta

Protein for Chicken RAR-beta

Protein for Chicken RAR-beta

Protein for Chicken RAR-beta

R&D Technical Data

s

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

s

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.2%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of RARB into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	92-103
Plasma	98	93-105

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RARB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-102%	92-101%	91-99%	93-100%
EDTA plasma(n=5)	94-103%	91-105%	92-106%	94-102%
heparin plasma(n=5)	93-105%	90-107%	95-101%	91-107%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Nucleotide sequence of an isoform of chicken retinoic acid binding protein-beta varying in its A domain."
Padanilam B.J. , McLeod L.B. , Suzuki H. , Solursh M.
Nucleic Acids Res.19:395-395(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA-1)
2.

"Isoforms of retinoic acid receptor beta expressed in the chicken embryo."
Nohno T. , Muto K. , Noji S. , Saito T. , Taniguchi S.
Biochim. Biophys. Acta1089:273-275(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS BETA-1 AND BETA-2)
3.

"Retinoic acid induces polarizing activity but is unlikely to be a morphogen in the chick limb bud."
Noji S. , Nohno T. , Koyama E. , Muto K. , Ohyama K. , Aoki Y. , Tamura K. , Ohsugi K. , Ide H. , Taniguchi S. , Saito T.
Nature350:83-86(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA-2);DEVELOPMENTAL STAGE
strain: White leghorn.
4.

"Temporal and regional differences in the expression pattern of distinct retinoic acid receptor-beta transcripts in the chick embryo."
Smith S.M. , Eichele G.
Development111:245-252(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA-2)
5.

"Differential expression of retinoic acid receptor-beta isoforms during chick limb ontogeny."
Smith S.M. , Kirstein I.J. , Wang Z.S. , Fallon J.F. , Kelley J. , Bradshaw-Rouse J.
Dev. Dyn.202:54-66(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-59 (ISOFORM BETA-1);FUNCTION;DEVELOPMENTAL STAGE
6.

"Retinoic acid treatment alters the distribution of retinoic acid receptor-beta transcripts in the embryonic chick face."
Rowe A. , Richman J.M. , Brickell P.M.
Development111:1007-1016(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-455 (ISOFORM BETA-2);INDUCTION;FUNCTION;DEVELOPMENTAL STAGE

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