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Index > Protein center > RARG(Gene name) > Human
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  • RARG (Gene name),
  • Retinoic acid receptor gamma (Protein name ),  RARG_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    RARG(NR1B3);
    Protein name:
    Retinoic acid receptor gamma(RAR-gamma);
    Alternative:
    Nuclear receptor subfamily 1 group B member 3;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Homodimer (By similarity). Heterodimer with a RXR molecule (By similarity). Binds DNA preferentially as a RAR/RXR heterodimer (By similarity). Forms a complex with PUS1 and the SRA1 RNA in the nucleus.
    Function:
    Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function.
    Subcellular Location:
    Nucleus
    Protein Attributes:
    Sequence length:
    454
    Sequence:
    50:
    MATNKERLFA | AGALGPGSGY | PGAGFPFAFP | GALRGSPPFE | MLSPSFRGLG | 
    100:
    QPDLPKEMAS | LSVETQSTSS | EEMVPSSPSP | PPPPRVYKPC | FVCNDKSSGY | 
    150:
    HYGVSSCEGC | KGFFRRSIQK | NMVYTCHRDK | NCIINKVTRN | RCQYCRLQKC | 
    200:
    FEVGMSKEAV | RNDRNKKKKE | VKEEGSPDSY | ELSPQLEELI | TKVSKAHQET | 
    250:
    FPSLCQLGKY | TTNSSADHRV | QLDLGLWDKF | SELATKCIIK | IVEFAKRLPG | 
    300:
    FTGLSIADQI | TLLKAACLDI | LMLRICTRYT | PEQDTMTFSD | GLTLNRTQMH | 
    350:
    NAGFGPLTDL | VFAFAGQLLP | LEMDDTETGL | LSAICLICGD | RMDLEEPEKV | 
    400:
    DKLQEPLLEA | LRLYARRRRP | SQPYMFPRML | MKITDLRGIS | TKGAERAITL | 
    450:
    KMEIPGPMPP | LIREMLENPE | MFEDDSSQPG | PHPNASSEDE | VPGGQGKGGL | 
    454:
    KSPA
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    KEGG:
    UniGene:
    MIM:
    Pfam:
    SMR:
    String:
    Uniprot:
     
    FOR
    ELISA Kit for Human RAR-gamma
    Cat.:
    E10643h
    Price:
    Please sign in first.
    MSDS:
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    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human RAR-gamma
    Cat.:
    E10643m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human RAR-gamma
    Cat.:
    U10643m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human RAR-gamma
    Cat.:
    U10643h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human RAR-gamma
    Cat.:
    P10643Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human RAR-gamma
    Cat.:
    P10643Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human RAR-gamma
    Monoclonal Antibody for Human RAR-gamma
    Protein for Human RAR-gamma
    Protein for Human RAR-gamma

    R&D Technical Data
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    s

    s
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Precision
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.7%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤8.1%

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Recovery
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Recovery was determined by spiking various levels of RARG into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    95

    91-103

    Plasma

    97

    93-104

     

     

     

     

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Linearity
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RARG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    90-99%

    90-102%

    88-103%

    92-98%

    EDTA plasma(n=5)

    89-106%

    93-100%

    90-105%

    92-101%

    heparin plasma(n=5)

    93-103%

     

    94-102%

    91-103%

    95-100%

     

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    References
    1. 1.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    2. 2.
      "A functional retinoic acid receptor encoded by the gene on human chromosome 12."
      Ishikawa T. , Umesono K. , Mangelsdorf D.J. , Aburatani H. , Stanger B.Z. , Shibasaki Y. , Imawari M. , Evans R.M. , Takaku F.
      Mol. Endocrinol.4:837-844(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    3. 3.
      "Genomic organization of the retinoic acid receptor gamma gene."
      Lehmann J.M. , Hoffmann B. , Pfahl M.
      Nucleic Acids Res.19:573-578(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);ALTERNATIVE SPLICING
      tissue: Placenta.
    4. 4.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4)
      tissue: Brain.
      tissue: Tongue.
      tissue: Umbilical cord blood.
    5. 5.
      "The finished DNA sequence of human chromosome 12."
      Scherer S.E. , Muzny D.M. , Buhay C.J. , Chen R. , Cree A. , Ding Y. , Dugan-Rocha S. , Gill R. , Gunaratne P. , Harris R.A. , Hawes A.C. , Hernandez J. , Hodgson A.V. , Hume J. , Jackson A. , Khan Z.M. , Kovar-Smith C. , Lewis L.R. , more...
      Nature440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    6. 6.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    7. 7.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      tissue: Colon.
    8. 8.
      "Murine isoforms of retinoic acid receptor gamma with specific patterns of expression."
      Kastner P. , Krust A. , Mendelsohn C. , Garnier J.-M. , Zelent A. , Leroy P. , Staub A. , Chambon P.
      Proc. Natl. Acad. Sci. U.S.A.87:2700-2704(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-63 (ISOFORM 2)
    9. 9.
      "RAR-gamma-2 expression is regulated through a retinoic acid response element embedded in Sp1 sites."
      Lehmann J.M. , Zhang X.K. , Pfahl M.
      Mol. Cell. Biol.12:2976-2985(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-55;ALTERNATIVE SPLICING
      tissue: Placenta.
    10. 10.
      Gebhard W.
      Submitted (1999-11) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 74-454
      tissue: Lung carcinoma.
    11. 11.
      "Genomic organization of the human retinoic acid receptor gamma gene."
      Xu H. , Clifford J.L.
      Submitted (2001-01) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 134-191 AND 394-411
    12. 12.
      "Crystal structure of the RAR-gamma ligand-binding domain bound to all-trans retinoic acid."
      Renaud J.-P. , Rochel N. , Ruff M. , Vivat V. , Chambon P. , Gronemeyer H. , Moras D.
      Nature378:681-689(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 178-423
    13. 13.
      "Conformational adaptation of agonists to the human nuclear receptor RAR gamma."
      Klaholz B.P. , Renaud J.-P. , Mitschler A. , Zusi C. , Chambon P. , Gronemeyer H. , Moras D.
      Nat. Struct. Biol.5:199-202(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 178-423
    14. 14.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT [LARGE SCALE ANALYSIS] SER-430
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