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Index > Protein center > RFWD2(Gene name) > Human
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  • RFWD2 (Gene name),
  • E3 ubiquitin-protein ligase RFWD2 (Protein name ),  RFWD2_HUMAN from NCBI database.
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  • General Annotation
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  • Gene name:
    RFWD2(COP1;RNF200);
    Protein name:
    E3 ubiquitin-protein ligase RFWD2;
    Alternative:
    RING finger and WD repeat domain protein 2;Constitutive photomorphogenesis protein 1 homolog(hCOP1);RING finger protein 200;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Homodimer. Homodimerization is mediated by the coiled coil domain. Component of the DCX DET1-COP1 ubiquitin ligase complex at least composed of RBX1, DET1, DDB1, CUL4A and COP1. Isoform 2 does not interact with CUL4A but still binds to RBX1, suggesting that the interaction may be mediated by another culllin protein. Isoform 1 and isoform 2 interact with CUL5 but not with CUL1, CUL2 not CUL3. Interacts with bZIP transcription factors JUN, JUNB and JUND but not with FOS, ATF2 nor XBP1. Interacts with p53 (TP53).
    Function:
    E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.
    Subcellular Location:
    Nucleus speckle Cytoplasm In the nucleus, it forms nuclear speckles.
    Protein Attributes:
    Sequence length:
    731
    Sequence:
    50:
    MSGSRQAGSG | SAGTSPGSSA | ASSVTSASSS | LSSSPSPPSV | AVSAAALVSG | 
    100:
    GVAQAAGSGG | LGGPVRPVLV | APAVSGSGGG | AVSTGLSRHS | CAARPSAGVG | 
    150:
    GSSSSLGSGS | RKRPLLAPLC | NGLINSYEDK | SNDFVCPICF | DMIEEAYMTK | 
    200:
    CGHSFCYKCI | HQSLEDNNRC | PKCNYVVDNI | DHLYPNFLVN | ELILKQKQRF | 
    250:
    EEKRFKLDHS | VSSTNGHRWQ | IFQDWLGTDQ | DNLDLANVNL | MLELLVQKKK | 
    300:
    QLEAESHAAQ | LQILMEFLKV | ARRNKREQLE | QIQKELSVLE | EDIKRVEEMS | 
    350:
    GLYSPVSEDS | TVPQFEAPSP | SHSSIIDSTE | YSQPPGFSGS | SQTKKQPWYN | 
    400:
    STLASRRKRL | TAHFEDLEQC | YFSTRMSRIS | DDSRTASQLD | EFQECLSKFT | 
    450:
    RYNSVRPLAT | LSYASDLYNG | SSIVSSIEFD | RDCDYFAIAG | VTKKIKVYEY | 
    500:
    DTVIQDAVDI | HYPENEMTCN | SKISCISWSS | YHKNLLASSD | YEGTVILWDG | 
    550:
    FTGQRSKVYQ | EHEKRCWSVD | FNLMDPKLLA | SGSDDAKVKL | WSTNLDNSVA | 
    600:
    SIEAKANVCC | VKFSPSSRYH | LAFGCADHCV | HYYDLRNTKQ | PIMVFKGHRK | 
    650:
    AVSYAKFVSG | EEIVSASTDS | QLKLWNVGKP | YCLRSFKGHI | NEKNFVGLAS | 
    700:
    NGDYIACGSE | NNSLYLYYKG | LSKTLLTFKF | DTVKSVLDKD | RKEDDTNEFV | 
    731:
    SAVCWRALPD | GESNVLIAAN | SQGTIKVLEL | V
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    SMR:
    Pfam:
    UniGene:
    MIM:
    String:
    KEGG:
    Uniprot:
     
    FOR
    ELISA Kit for Human E3 ubiquitin-protein ligase RFWD2
    ELISA Kit for Human E3 ubiquitin-protein ligase RFWD2
    CLIA Kit for Human E3 ubiquitin-protein ligase RFWD2
    CLIA Kit for Human E3 ubiquitin-protein ligase RFWD2
    Polyclonal Antibody for Human E3 ubiquitin-protein ligase RFWD2
    Polyclonal Antibody for Human E3 ubiquitin-protein ligase RFWD2
    Monoclonal Antibody for Human E3 ubiquitin-protein ligase RFWD2
    Monoclonal Antibody for Human E3 ubiquitin-protein ligase RFWD2
    Protein for Human E3 ubiquitin-protein ligase RFWD2
    Protein for Human E3 ubiquitin-protein ligase RFWD2

    R&D Technical Data
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    Precision
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    Recovery
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    Linearity
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    References
    1. 1.
      "An initial biochemical and cell biological characterization of the mammalian homologue of a central plant developmental switch, COP1."
      Yi C. , Wang H. , Wei N. , Deng X.-W.
      BMC Cell Biol.3:30-30(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1);FUNCTION;HOMODIMERIZATION;SUBCELLULAR LOCATION
    2. 2.
      "Characterization of human constitutive photomorphogenesis protein 1, a RING finger ubiquitin ligase that interacts with Jun transcription factors and modulates their transcriptional activity."
      Bianchi E. , Denti S. , Catena R. , Rossetti G. , Polo S. , Gasparian S. , Putignano S. , Rogge L. , Pardi R.
      J. Biol. Chem.278:19682-19690(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);FUNCTION;SUBCELLULAR LOCATION;TISSUE SPECIFICITY;INTERACTION WITH JUN; JUNB AND JUND;MUTAGENESIS OF 111-ARG--ARG-113; CYS-136 AND CYS-139
    3. 3.
      "Human De-etiolated-1 regulates c-Jun by assembling a CUL4A ubiquitin ligase."
      Wertz I.E. , O'Rourke K.M. , Zhang Z. , Dornan D. , Arnott D. , Deshaies R.J. , Dixit V.M.
      Science303:1371-1374(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2);IDENTIFICATION BY MASS SPECTROMETRY;FUNCTION;ALTERNATIVE SPLICING;COMPONENT OF THE DCX DET1-COP1 COMPLEX WITH RBX1; CUL4A; DET1 AND DDB1;MUTAGENESIS OF CYS-136 AND CYS-139
    4. 4.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 5)
      tissue: Brain.
    5. 5.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3)
      tissue: Hepatoma.
      tissue: Spleen.
    6. 6.
      "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G. , Barlow K.F. , McLay K.E. , Kaul R. , Swarbreck D. , Dunham A. , Scott C.E. , Howe K.L. , Woodfine K. , Spencer C.C.A. , Jones M.C. , Gillson C. , Searle S. , Zhou Y. , Kokocinski F. , McDonald L. , Evans R. , Phillips K. , more...
      Nature441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    7. 7.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    8. 8.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      tissue: Testis.
    9. 9.
      "The ubiquitin ligase COP1 is a critical negative regulator of p53."
      Dornan D. , Wertz I. , Shimizu H. , Arnott D. , Frantz G.D. , Dowd P. , O'Rourke K. , Koeppen H. , Dixit V.M.
      Nature429:86-92(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;TISSUE SPECIFICITY;INDUCTION;INTERACTION WITH TP53
    10. 10.
      "COP1D, an alternatively spliced constitutive photomorphogenic-1 (COP1) product, stabilizes UV stress-induced c-Jun through inhibition of full-length COP1."
      Savio M.G. , Rotondo G. , Maglie S. , Rossetti G. , Bender J.R. , Pardi R.
      Oncogene27:2401-2411(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ALTERNATIVE SPLICING (ISOFORM 4);SUBUNIT
    11. 11.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH TP53
    12. 12.
      "E3 ubiquitin ligase COP1 regulates the stability and functions of MTA1."
      Li D.Q. , Ohshiro K. , Reddy S.D. , Pakala S.B. , Lee M.H. , Zhang Y. , Rayala S.K. , Kumar R.
      Proc. Natl. Acad. Sci. U.S.A.106:17493-17498(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH MTA1;MUTAGENESIS OF CYS-136; CYS-139; CYS-156 AND CYS-159;AUTOUBIQUITINATION
    13. 13.
      "COP9 signalosome subunit 6 stabilizes COP1, which functions as an E3 ubiquitin ligase for 14-3-3sigma."
      Choi H.H. , Gully C. , Su C.H. , Velazquez-Torres G. , Chou P.C. , Tseng C. , Zhao R. , Phan L. , Shaiken T. , Chen J. , Yeung S.C. , Lee M.H.
      Oncogene30:4791-4801(2011) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH COPS6 AND SFN;MUTAGENESIS OF CYS-136 AND CYS-139
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