ROCK2 ELISA Kit for Human(Homo sapiens) | EIAab.comROCK2 ELISA Kit for Human(Homo sapiens)

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ROCK2 (Gene name),

Rho-associated protein kinase 2 (Protein name ), ROCK2_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

ROCK2(KIAA0619);

Protein name:

Rho-associated protein kinase 2;

Alternative:

Rho-associated, coiled-coil-containing protein kinase 2;Rho kinase 2;p164 ROCK-2;Rho-associated, coiled-coil-containing protein kinase II(ROCK-II);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homodimer. Interacts with IRS1, RHOB and RHOC (By similarity). Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity.

Function:

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK (RPS6KA1) during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus.

Subcellular Location:

Cytoplasm Cell membrane Peripheral membrane protein Nucleus Cytoplasm cytoskeleton centrosome Cytoplasmic, and associated with actin microfilaments and the plasma membrane.

Protein Attributes:

Sequence length:

1388

Sequence:

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1150:

1200:

1250:

1300:

1350:

1388:

KPPAPDPFAR | SSPRTSMKIQ | QNQSIRRPSR | QLAPNKPS

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

MIM:

604002

STRING:

9606.ENSP00000317985

SMR:

O75116

KEGG:

hsa:9475

UniGene:

Hs.681743

String:

O75116

UniGene:

Hs.58617

Pfam:

PF02185

Uniprot:

O75116

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ELISA Kit for Human Rho-associated protein kinase 2

Cat.:

E1852h

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96T

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ELISA Kit for Human Rho-associated protein kinase 2

Cat.:

E1852m

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96T

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ELISA Kit for Human Rho-associated protein kinase 2

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E1852r

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96T

ELISA Kit for Human Rho-associated protein kinase 2

Cat.:

E1852b

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96T

CLIA Kit for Human Rho-associated protein kinase 2

Cat.:

U1852b

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96T

CLIA Kit for Human Rho-associated protein kinase 2

Cat.:

U1852h

Price:

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

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CLIA Kit for Human Rho-associated protein kinase 2

Cat.:

U1852m

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MSDS:

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Packing:

96T

CLIA Kit for Human Rho-associated protein kinase 2

Cat.:

U1852r

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96T

Polyclonal Antibody for Human Rho-associated protein kinase 2

Cat.:

P1852Rb-m

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40ug/0.2ml

Polyclonal Antibody for Human Rho-associated protein kinase 2

Cat.:

P1852Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human Rho-associated protein kinase 2

Cat.:

P1852Rb-h

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40ug/0.2ml

Monoclonal Antibody for Human Rho-associated protein kinase 2

Protein for Human Rho-associated protein kinase 2

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

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Recovery

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Linearity

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References

1.

"Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24."
Takahashi N. , Tuiki H. , Saya H. , Kaibuchi K.
Genomics55:235-237(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANT ASN-431
tissue: Brain.
2.

"Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
Ishikawa K. , Nagase T. , Suyama M. , Miyajima N. , Tanaka A. , Kotani H. , Nomura N. , Ohara O.
DNA Res.5:169-176(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANT ASN-431
tissue: Brain.
3.

"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W. , Graves T.A. , Fulton R.S. , Fulton L.A. , Pepin K.H. , Minx P. , Wagner-McPherson C. , Layman D. , Wylie K. , Sekhon M. , Becker M.C. , Fewell G.A. , Delehaunty K.D. , Miner T.L. , Nash W.E. , Kremitzki C. , Oddy L. , Du H. , more...
Nature434:724-731(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
4.

"Phosphorylation of myosin-binding subunit (MBS) of myosin phosphatase by Rho-kinase in vivo."
Kawano Y. , Fukata Y. , Oshiro N. , Amano M. , Nakamura T. , Ito M. , Matsumura F. , Inagaki M. , Kaibuchi K.
J. Cell Biol.147:1023-1038(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH PPP1R12A
5.

"Direct cleavage of ROCK II by granzyme B induces target cell membrane blebbing in a caspase-independent manner."
Sebbagh M. , Hamelin J. , Bertoglio J. , Solary E. , Breard J.
J. Exp. Med.201:465-471(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CLEAVAGE BY GRANZYME B;MUTAGENESIS OF ASP-1131;FUNCTION
6.

"Nuclear Rho kinase, ROCK2, targets p300 acetyltransferase."
Tanaka T. , Nishimura D. , Wu R.C. , Amano M. , Iso T. , Kedes L. , Nishida H. , Kaibuchi K. , Hamamori Y.
J. Biol. Chem.281:15320-15329(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;INTERACTION WITH EP300
7.

"Interaction between ROCK II and nucleophosmin/B23 in the regulation of centrosome duplication."
Ma Z. , Kanai M. , Kawamura K. , Kaibuchi K. , Ye K. , Fukasawa K.
Mol. Cell. Biol.26:9016-9034(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;INTERACTION WITH NPM1
8.

"Regulation of RhoA-dependent ROCKII activation by Shp2."
Lee H.H. , Chang Z.F.
J. Cell Biol.181:999-1012(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT TYR-722;DEPHOSPHORYLATION
9.

"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H. , Olsen J.V. , Bairlein M. , Gnad F. , Oppermann F.S. , Korner R. , Greff Z. , Keri G. , Stemmann O. , Mann M.
Mol. Cell31:438-448(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1137;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
10.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1137;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
11.

"ROCK isoform regulation of myosin phosphatase and contractility in vascular smooth muscle cells."
Wang Y. , Zheng X.R. , Riddick N. , Bryden M. , Baur W. , Zhang X. , Surks H.K.
Circ. Res.104:531-540(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH PPP1R12A
12.

"Distinct roles for ROCK1 and ROCK2 in the regulation of keratinocyte differentiation."
Lock F.E. , Hotchin N.A.
PLoS ONE4:E8190-E8190(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
13.

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1137;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Leukemic T-cell.
14.

"Morgana/chp-1, a ROCK inhibitor involved in centrosome duplication and tumorigenesis."
Ferretti R. , Palumbo V. , Di Savino A. , Velasco S. , Sbroggio M. , Sportoletti P. , Micale L. , Turco E. , Silengo L. , Palumbo G. , Hirsch E. , Teruya-Feldstein J. , Bonaccorsi S. , Pandolfi P.P. , Gatti M. , Tarone G. , Brancaccio M.
Dev. Cell18:486-495(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CHORDC1
15.

"Src-dependent phosphorylation of ROCK participates in regulation of focal adhesion dynamics."
Lee H.H. , Tien S.C. , Jou T.S. , Chang Y.C. , Jhong J.G. , Chang Z.F.
J. Cell Sci.123:3368-3377(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT TYR-722
16.

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1137;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
17.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
18.

"BRCA2 and nucleophosmin coregulate centrosome amplification and form a complex with the Rho effector kinase ROCK2."
Wang H.F. , Takenaka K. , Nakanishi A. , Miki Y.
Cancer Res.71:68-77(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH BRCA2
19.

"Rho kinase II phosphorylation of the lipoprotein receptor LR11/SORLA alters amyloid-beta production."
Herskowitz J.H. , Seyfried N.T. , Gearing M. , Kahn R.A. , Peng J. , Levey A.I. , Lah J.J.
J. Biol. Chem.286:6117-6127(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH SORL1
20.

"Rocks: multifunctional kinases in cell behaviour."
Riento K. , Ridley A.J.
Nat. Rev. Mol. Cell Biol.4:446-456(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
21.

"Rho-kinase/ROCK: A key regulator of the cytoskeleton and cell polarity."
Amano M. , Nakayama M. , Kaibuchi K.
Cytoskeleton67:545-554(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
22.

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T. , Prokhorova T.A. , Akimov V. , Henningsen J. , Johansen P.T. , Kratchmarova I. , Kassem M. , Mann M. , Olsen J.V. , Blagoev B.
Sci. Signal.4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1137;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
23.

"Patterns of somatic mutation in human cancer genomes."
Greenman C. , Stephens P. , Smith R. , Dalgliesh G.L. , Hunter C. , Bignell G. , Davies H. , Teague J. , Butler A. , Stevens C. , Edkins S. , O'Meara S. , Vastrik I. , Schmidt E.E. , Avis T. , Barthorpe S. , Bhamra G. , Buck G. , more...
Nature446:153-158(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS [LARGE SCALE ANALYSIS] ASN-431; VAL-601 AND PRO-1194

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