RPTOR ELISA Kit for Human(Homo sapiens) | EIAab.comRPTOR ELISA Kit for Human(Homo sapiens)

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RPTOR (Gene name),

Regulatory-associated protein of mTOR (Protein name ), RPTOR_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

RPTOR(KIAA1303;RAPTOR);

Protein name:

Regulatory-associated protein of mTOR(Raptor);

Alternative:

p150 target of rapamycin (TOR)-scaffold protein;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR and AKT1S1. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Binds directly to 4EBP1 and RPS6KB1 independently of its association with MTOR. Binds preferentially to poorly or non-phosphorylated forms of EIF4EBP1, and this binding is critical to the ability of MTOR to catalyze phosphorylation. Forms a complex with MTOR under both leucine-rich and -poor conditions. Interacts with ULK1 in a nutrient-dependent manner; the interaction is reduced during starvation. Interacts (when phosphorylated by AMPK) with 14-3-3 protein, leading to inhibit its activity.

Function:

Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.

Subcellular Location:

Cytoplasm Lysosome Targeting to lysosomes depends on amino acid availability.

Protein Attributes:

Sequence length:

1335

Sequence:

50:

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1050:

1100:

1150:

1200:

1250:

1300:

1335:

VGAISCLAFH | PHWPHLAVGS | NDYYISVYSV | EKRVR

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

SMR:

Q8N122

Pfam:

PF02985

MIM:

607130

STRING:

9606.ENSP00000307272

UniGene:

Hs.133044

String:

Q8N122

KEGG:

hsa:57521

Uniprot:

Q8N122

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ELISA Kit for Human Raptor

Cat.:

E1552h

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96T

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ELISA Kit for Human Raptor

Cat.:

E1552m

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Manual:

Looking for manual.

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Packing:

96T

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CLIA Kit for Human Raptor

Cat.:

U1552h

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Human Raptor

Cat.:

U1552m

Price:

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MSDS:

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Packing:

96T

Polyclonal Antibody for Human Raptor

Cat.:

P1552Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human Raptor

Cat.:

P1552Rb-h

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human Raptor

Protein for Human Raptor

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Characterization of a novel splicing variant in the RAPTOR gene."
Sun C. , Southard C. , Di Rienzo A.
Mutat. Res.662:88-92(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 488-594 (ISOFORM 3);TISSUE SPECIFICITY (ISOFORM 3)
2.

"mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the growth machinery."
Kim D.-H. , Sarbassov D.D. , Ali S.M. , King J.E. , Latek R.R. , Erdjument-Bromage H. , Tempst P. , Sabatini D.M.
Cell110:163-175(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];FUNCTION;TISSUE SPECIFICITY;INTERACTION WITH 4EBP1 AND RPS6KB1
3.

"Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action."
Hara K. , Maruki Y. , Long X. , Yoshino K. , Oshiro N. , Hidayat S. , Tokunaga C. , Avruch J. , Yonezawa K.
Cell110:177-189(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];FUNCTION;INTERACTION WITH 4EBP1 AND RPS6KB1
4.

"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C. , Garber M. , Adams D.J. , Sharpe T. , Harrow J. , Lupski J.R. , Nicholson C. , Searle S.M. , Wilming L. , Young S.K. , Abouelleil A. , Allen N.R. , Bi W. , Bloom T. , Borowsky M.L. , Bugalter B.E. , Butler J. , Chang J.L. , more...
Nature440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
5.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
6.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2);NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 995-1135 (ISOFORM 1)
tissue: Brain.
tissue: Placenta.
tissue: Testis.
7.

"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
Nagase T. , Kikuno R. , Ishikawa K. , Hirosawa M. , Ohara O.
DNA Res.7:65-73(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 217-1335
tissue: Brain.
8.

"Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control."
Loewith R. , Jacinto E. , Wullschleger S. , Lorberg A. , Crespo J.L. , Bonenfant D. , Oppliger W. , Jenoe P. , Hall M.N.
Mol. Cell10:457-468(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH MTOR AND MLST8;IDENTIFICATION IN THE TORC1 COMPLEX;TISSUE SPECIFICITY
9.

"TOS motif-mediated raptor binding regulates 4E-BP1 multisite phosphorylation and function."
Schalm S.S. , Fingar D.C. , Sabatini D.M. , Blenis J.
Curr. Biol.13:797-806(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH EIF4EBP1
10.

"Dissociation of raptor from mTOR is a mechanism of rapamycin-induced inhibition of mTOR function."
Oshiro N. , Yoshino K. , Hidayat S. , Tokunaga C. , Hara K. , Eguchi S. , Avruch J. , Yonezawa K.
Genes Cells9:359-366(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISSOCIATION OF COMPLEX BY RAPAMYCIN
11.

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V. , Blagoev B. , Gnad F. , Macek B. , Kumar C. , Mortensen P. , Mann M.
Cell127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
12.

"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A. , Villen J. , Gerber S.A. , Rush J. , Gygi S.P.
Nat. Biotechnol.24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-863;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
13.

"PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase."
Sancak Y. , Thoreen C.C. , Peterson T.R. , Lindquist R.A. , Kang S.A. , Spooner E. , Carr S.A. , Sabatini D.M.
Mol. Cell25:903-915(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH AKT1S1
14.

"Oncogenic MAPK signaling stimulates mTORC1 activity by promoting RSK-mediated raptor phosphorylation."
Carriere A. , Cargnello M. , Julien L.A. , Gao H. , Bonneil E. , Thibault P. , Roux P.P.
Curr. Biol.18:1269-1277(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-719; SER-721 AND SER-722
15.

"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T. , Yi W. , Lu B. , Park S.K. , Xu T. , Lee J.-D. , Yates J.R. III
J. Proteome Res.7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
16.

"AMPK phosphorylation of raptor mediates a metabolic checkpoint."
Gwinn D.M. , Shackelford D.B. , Egan D.F. , Mihaylova M.M. , Mery A. , Vasquez D.S. , Turk B.E. , Shaw R.J.
Mol. Cell30:214-226(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-722 AND SER-792;MUTAGENESIS OF SER-722 AND SER-792;INTERACTION WITH 14-3-3
17.

"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H. , Olsen J.V. , Bairlein M. , Gnad F. , Oppermann F.S. , Korner R. , Greff Z. , Keri G. , Stemmann O. , Mann M.
Mol. Cell31:438-448(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-877;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
18.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-719; SER-859; SER-863 AND SER-877;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
19.

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
20.

"Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy."
Hosokawa N. , Hara T. , Kaizuka T. , Kishi C. , Takamura A. , Miura Y. , Iemura S. , Natsume T. , Takehana K. , Yamada N. , Guan J.L. , Oshiro N. , Mizushima N.
Mol. Biol. Cell20:1981-1991(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ULK1
21.

"Large-scale proteomics analysis of the human kinome."
Oppermann F.S. , Gnad F. , Olsen J.V. , Hornberger R. , Greff Z. , Keri G. , Mann M. , Daub H.
Mol. Cell. Proteomics8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-877;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
22.

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-859; SER-863 AND SER-877;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Leukemic T-cell.
23.

"Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids."
Sancak Y. , Bar-Peled L. , Zoncu R. , Markhard A.L. , Nada S. , Sabatini D.M.
Cell141:290-303(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION
24.

"Regulation of mTOR complex 1 (mTORC1) by raptor Ser863 and multisite phosphorylation."
Foster K.G. , Acosta-Jaquez H.A. , Romeo Y. , Ekim B. , Soliman G.A. , Carriere A. , Roux P.P. , Ballif B.A. , Fingar D.C.
J. Biol. Chem.285:80-94(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-696; THR-706; SER-855; SER-859; SER-863 AND SER-877
25.

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-859; SER-863 AND SER-877;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
26.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
27.

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T. , Prokhorova T.A. , Akimov V. , Henningsen J. , Johansen P.T. , Kratchmarova I. , Kassem M. , Mann M. , Olsen J.V. , Blagoev B.
Sci. Signal.4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-863 AND SER-877;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
28.

"5-HT(6) receptor recruitment of mTOR as a mechanism for perturbed cognition in schizophrenia."
Meffre J. , Chaumont-Dubel S. , Mannoury la Cour C. , Loiseau F. , Watson D.J. , Dekeyne A. , Seveno M. , Rivet J.M. , Gaven F. , Deleris P. , Herve D. , Fone K.C. , Bockaert J. , Millan M.J. , Marin P.
EMBO Mol. Med.4:1043-1056(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HTR6
29.

"Osmotic stress regulates mammalian target of rapamycin (mTOR) complex 1 via c-Jun N-terminal Kinase (JNK)-mediated Raptor protein phosphorylation."
Kwak D. , Choi S. , Jeong H. , Jang J.H. , Lee Y. , Jeon H. , Lee M.N. , Noh J. , Cho K. , Yoo J.S. , Hwang D. , Suh P.G. , Ryu S.H.
J. Biol. Chem.287:18398-18407(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-696; THR-706 AND SER-863
30.

"Inhibition of mTORC1 by astrin and stress granules prevents apoptosis in cancer cells."
Thedieck K. , Holzwarth B. , Prentzell M.T. , Boehlke C. , Klasener K. , Ruf S. , Sonntag A.G. , Maerz L. , Grellscheid S.N. , Kremmer E. , Nitschke R. , Kuehn E.W. , Jonker J.W. , Groen A.K. , Reth M. , Hall M.N. , Baumeister R.
Cell154:859-874(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH G3BP1 AND SPAG5;SUBCELLULAR LOCATION
31.

"The Bardet-Biedl syndrome-related protein CCDC28B modulates mTORC2 function and interacts with SIN1 to control cilia length independently of the mTOR complex."
Cardenas-Rodriguez M. , Irigoin F. , Osborn D.P. , Gascue C. , Katsanis N. , Beales P.L. , Badano J.L.
Hum. Mol. Genet.22:4031-4042(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN CILIOGENESIS

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