Sepp1 ELISA Kit for Rat(Rattus norvegicus) | EIAab.comSepp1 ELISA Kit for Rat(Rattus norvegicus)

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Index > Protein center > Sepp1(Gene name) > Rat

Sepp1 (Gene name),

Selenoprotein P (Protein name ), SEPP1_RAT from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Sepp1(Selp);

Protein name:

Selenoprotein P(SeP);

Alternative:

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

N/A

Function:

Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

385

Sequence:

50:

100:

150:

200:

250:

300:

350:

385:

RSPPAAUHSQ | HVSPTEASPN | USUNNKTKKU | KUNLN

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF04593

KEGG:

rno:29360

UniGene:

Rn.1451

String:

P25236

STRING:

10116.ENSRNOP00000021292

Uniprot:

P25236

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ELISA Kit for Rat SeP

Cat.:

E1809h

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Packing:

96T

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ELISA Kit for Rat SeP

Cat.:

E1809m

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Packing:

96T

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ELISA Kit for Rat SeP

Cat.:

E1809b

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Packing:

96T

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ELISA Kit for Rat SeP

Cat.:

E1809r

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Packing:

96T

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CLIA Kit for Rat SeP

Cat.:

U1809m

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Packing:

96T

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CLIA Kit for Rat SeP

Cat.:

U1809h

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Packing:

96T

Range:

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CLIA Kit for Rat SeP

Cat.:

U1809r

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Packing:

96T

CLIA Kit for Rat SeP

Cat.:

U1809b

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MSDS:

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Packing:

96T

Polyclonal Antibody for Rat SeP

Cat.:

P1809Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat SeP

Cat.:

P1809Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat SeP

Cat.:

P1809Rb-r

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Packing:

40ug/0.2ml

Monoclonal Antibody for Rat SeP

Protein for Rat SeP

Cat.:

R1809h

Price:

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Packing:

1mg

Purity:

>90%

Protein for Rat SeP

R&D Technical Data

12% SDS-PAGE analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.1%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of SEPP1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	94	91-98
Plasma	96	93-100

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SEPP1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-98%	90-97%	96-100%	91-97%
EDTA plasma(n=5)	89-94%	93-99%	90-97%	95-101%
heparin plasma(n=5)	93-97%	94-99%	96-102%	91-94%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"The cDNA for rat selenoprotein P contains 10 TGA codons in the open reading frame."
Hill K.E. , Lloyd R.S. , Yang J.-G. , Read R. , Burk R.F.
J. Biol. Chem.266:10050-10053(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 20-41; 267-287 AND 316-327
tissue: Liver.
2.

"Molecular cloning of cDNA encoding a bovine selenoprotein P-like protein containing 12 selenocysteines and a (His-Pro) rich domain insertion, and its regional expression."
Saijoh K. , Saito N. , Lee M.J. , Fujii M. , Kobayashi T. , Sumino K.
Brain Res. Mol. Brain Res.30:301-311(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Wistar.
tissue: Kidney.
3.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Heart.
4.

"Mass spectrometric characterization of full-length rat selenoprotein P and three isoforms shortened at the C-terminus. Evidence that three UGA codons in the mRNA open reading frame have alternative functions of specifying selenocysteine insertion or translation termination."
Ma S. , Hill K.E. , Caprioli R.M. , Burk R.F.
J. Biol. Chem.277:12749-12754(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION OF ISOFORMS
5.

"Mass spectrometric identification of N- and O-glycosylation sites of full-length rat selenoprotein P and determination of selenide-sulfide and disulfide linkages in the shortest isoform."
Ma S. , Hill K.E. , Burk R.F. , Caprioli R.M.
Biochemistry42:9703-9711(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT ASN-83; ASN-174; ASN-188 AND THR-365;ABSENCE OF GLYCOSYLATION AT ASN-370 AND ASN-375;DISULFIDE BONDS
6.

"Selenoprotein P. A selenium-rich extracellular glycoprotein."
Burk R.F. , Hill K.E.
J. Nutr.124:1891-1897(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW

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