SMURF2 ELISA Kit for Human(Homo sapiens) | EIAab.comSMURF2 ELISA Kit for Human(Homo sapiens)

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SMURF2 (Gene name),

E3 ubiquitin-protein ligase SMURF2 (Protein name ), SMUF2_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

SMURF2;

Protein name:

E3 ubiquitin-protein ligase SMURF2(hSMURF2);

Alternative:

SMAD-specific E3 ubiquitin-protein ligase 2;SMAD ubiquitination regulatory factor 2;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Interacts (via WW domains) with SMAD1. Interacts (via WW domains) with SMAD2 (via PY-motif). Interacts (via WW domains) with SMAD3 (via PY-motif). Interacts with SMAD6. Interacts with SMAD7 (via PY-motif) and TGFBR1; SMAD7 recruits SMURF2 to the TGF-beta receptor and regulates its degradation. Does not interact with SMAD4; SMAD4 lacks a PY-motif. Interacts with AIMP1. Interacts with STAMBP and RNF11. Interacts with NDFIP1 and NDFIP2 (Probable); this interaction activates the E3 ubiquitin-protein ligase.

Function:

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.

Subcellular Location:

Nucleus Cytoplasm Cell membrane Membrane raft Cytoplasmic in the presence of SMAD7. Co-localizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.

Protein Attributes:

Sequence length:

748

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

748:

HQIDACTNNL | PKAHTCFNRI | DIPPYESYEK | LYEKLLTAIE | ETCGFAVE

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

Q9HAU4

SMR:

Q9HAU4

Pfam:

PF00168

UniGene:

Hs.515011

KEGG:

hsa:64750

MIM:

605532

STRING:

9606.ENSP00000262435

Uniprot:

Q9HAU4

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ELISA Kit for Human hSMURF2

Cat.:

E2373h

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Manual:

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MSDS:

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Packing:

96T

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ELISA Kit for Human hSMURF2

Cat.:

E2373m

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MSDS:

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Packing:

96T

CLIA Kit for Human hSMURF2

Cat.:

U2373h

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MSDS:

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Packing:

96T

CLIA Kit for Human hSMURF2

Cat.:

U2373m

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MSDS:

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Packing:

96T

Polyclonal Antibody for Human hSMURF2

Cat.:

P2373Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human hSMURF2

Cat.:

P2373Rb-h

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human hSMURF2

Protein for Human hSMURF2

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Smad7 binds to Smurf2 to form an E3 ubiquitin ligase that targets the TGF-beta receptor for degradation."
Kavsak P. , Rasmussen R.K. , Causing C.G. , Bonni S. , Zhu H. , Thomsen G.H. , Wrana J.L.
Mol. Cell6:1365-1375(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];MUTAGENESIS OF 251-PRO--VAL-284 AND 297-GLY--LEU-330;INTERACTION WITH SMAD7 AND TGFBR1
2.

"Smurf2 Is a ubiquitin E3 ligase mediating proteasome-dependent degradation of Smad2 in transforming growth factor-beta signaling."
Lin X. , Liang M. , Feng X.-H.
J. Biol. Chem.275:36818-36822(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];MUTAGENESIS OF 251-PRO--VAL-284 AND CYS-716
3.

"Regulation of Smad degradation and activity by Smurf2, an E3 ubiquitin ligase."
Zhang Y. , Chang C. , Gehling D.J. , Hemmati-Brivanlou A. , Derynck R.
Proc. Natl. Acad. Sci. U.S.A.98:974-979(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];MUTAGENESIS OF CYS-716
4.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Brain.
5.

"TGF-beta induces assembly of a Smad2-Smurf2 ubiquitin ligase complex that targets SnoN for degradation."
Bonni S. , Wang H.R. , Causing C.G. , Kavsak P. , Stroschein S.L. , Luo K. , Wrana J.L.
Nat. Cell Biol.3:587-595(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH SMAD2; SMAD3 AND SNON
6.

"Distinct endocytic pathways regulate TGF-beta receptor signalling and turnover."
Di Guglielmo G.M. , Le Roy C. , Goodfellow A.F. , Wrana J.L.
Nat. Cell Biol.5:410-421(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;MUTAGENESIS OF CYS-716
7.

Di Guglielmo G.M. , Le Roy C. , Goodfellow A.F. , Wrana J.L.
Nat. Cell Biol.5:680-680(2003)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM
8.

"The RING-H2 protein RNF11 is overexpressed in breast cancer and is a target of Smurf2 E3 ligase."
Subramaniam V. , Li H. , Wong M.J. , Kitching R. , Attisano L. , Wrana J. , Zubovits J. , Burger A.M. , Seth A.K.
Br. J. Cancer89:1538-1544(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH RNF11
9.

"An RNF11: Smurf2 complex mediates ubiquitination of the AMSH protein."
Li H. , Seth A.K.
Oncogene23:1801-1808(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH STAMBP AND RNF11
10.

"AIMP1/p43 downregulates TGF-beta signaling via stabilization of smurf2."
Lee Y.S. , Han J.M. , Son S.H. , Choi J.W. , Jeon E.J. , Bae S.-C. , Park Y.I. , Kim S.
Biochem. Biophys. Res. Commun.371:395-400(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH AIMP1
11.

"Control of the activity of WW-HECT domain E3 ubiquitin ligases by NDFIP proteins."
Mund T. , Pelham H.R.
EMBO Rep.10:501-507(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACTIVATION BY NDFIP1 AND NDFIP2;AUTOUBIQUITINATION
12.

"SCF(FBXL15) regulates BMP signalling by directing the degradation of HECT-type ubiquitin ligase Smurf1."
Cui Y. , He S. , Xing C. , Lu K. , Wang J. , Xing G. , Meng A. , Jia S. , He F. , Zhang L.
EMBO J.30:2675-2689(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION
13.

"Regulation of Smurf2 ubiquitin ligase activity by anchoring the E2 to the HECT domain."
Ogunjimi A.A. , Briant D.J. , Pece-Barbara N. , Le Roy C. , Di Guglielmo G.M. , Kavsak P. , Rasmussen R.K. , Seet B.T. , Sicheri F. , Wrana J.L.
Mol. Cell19:297-308(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 369-748;INTERACTION WITH SMAD7;MUTAGENESIS OF TRP-535; HIS-547 AND TYR-581
14.

"An expanded WW domain recognition motif revealed by the interaction between Smad7 and the E3 ubiquitin ligase Smurf2."
Chong P.A. , Lin H. , Wrana J.L. , Forman-Kay J.D.
J. Biol. Chem.281:17069-17075(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 297-333 IN COMPLEX WITH SMAD7
15.

"Autoinhibition of the HECT-type ubiquitin ligase Smurf2 through its C2 domain."
Wiesner S. , Ogunjimi A.A. , Wang H.R. , Rotin D. , Sicheri F. , Wrana J.L. , Forman-Kay J.D.
Cell130:651-662(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 10-140;AUTOINHIBITION BY C2 DOMAIN;MUTAGENESIS OF PHE-29; PHE-30; THR-56 AND LEU-57

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