Protein name:
Serine/arginine-rich splicing factor 1 ;
Alternative:
Pre-mRNA-splicing factor SRp30a ;ASF/SF2 ;Splicing factor, arginine/serine-rich 1 ;
Organism:
Mouse (Mus musculus).
General Annotation
Sub Unit:
Consists of two polypeptides of p32 and p33. In vitro, self-associates and binds SRSF2, SNRNP70 and U2AF1 but not U2AF2. Binds SREK1/SFRS12. Interacts with SAFB/SAFB1. Interacts with SRPK1. Interacts with PSIP1/LEDGF. Identified in the spliceosome C complex. Interacts with RSRC1 (via Arg/Ser-rich domain). Interacts with ZRSR2/U2AF1-RS2. Interacts with CCDC55 (via C-terminus).
Function:
Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing (By similarity). Specifically regulates alternative splicing of cardiac isoforms of CAMK2D, LDB3/CYPHER and TNNT2/CTNT during heart remodeling at the juvenile to adult transition. The inappropriate accumulation of a neonatal and neuronal isoform of CAMKD2 in the adult heart results in aberrant calcium handling and defective excitation-contraction coupling in cardiomyocytes.
Subcellular Location:
Cytoplasm
Nucleus
Nucleus speckle
Shuttles between the nucleus and the cytoplasm.
Protein Attributes:
50:
MSGGGVIRGP | AGNNDCRIYV | GNLPPDIRTK | DIEDVFYKYG | AIRDIDLKNR |
100:
RGGPPFAFVE | FEDPRDAEDA | VYGRDGYDYD | GYRLRVEFPR | SGRGTGRGGG |
150:
GGGGGGAPRG | RYGPPSRRSE | NRVVVSGLPP | SGSWQDLKDH | MREAGDVCYA |
200:
DVYRDGTGVV | EFVRKEDMTY | AVRKLDNTKF | RSHEGETAYI | RVKVDGPRSP |
248:
SYGRSRSRSR | SRSRSRSRSN | SRSRSYSPRR | SRGSPRYSPR | HSRSRSRT
Vaild Sequence:
Related Databases
Uniprot:
ELISA Kit
CLIA Kit
Polyclonal Antibody
Monoclonal Antibody
Protein
FOR
Human
Chicken
Mouse
Pig
Bovine
ELISA Kit for Mouse Serine/arginine-rich splicing factor 1
ELISA Kit for Mouse Serine/arginine-rich splicing factor 1
ELISA Kit for Mouse Serine/arginine-rich splicing factor 1
ELISA Kit for Mouse Serine/arginine-rich splicing factor 1
ELISA Kit for Mouse Serine/arginine-rich splicing factor 1
CLIA Kit for Mouse Serine/arginine-rich splicing factor 1
CLIA Kit for Mouse Serine/arginine-rich splicing factor 1
CLIA Kit for Mouse Serine/arginine-rich splicing factor 1
CLIA Kit for Mouse Serine/arginine-rich splicing factor 1
CLIA Kit for Mouse Serine/arginine-rich splicing factor 1
Polyclonal Antibody for Mouse Serine/arginine-rich splicing factor 1
Polyclonal Antibody for Mouse Serine/arginine-rich splicing factor 1
Polyclonal Antibody for Mouse Serine/arginine-rich splicing factor 1
Polyclonal Antibody for Mouse Serine/arginine-rich splicing factor 1
Polyclonal Antibody for Mouse Serine/arginine-rich splicing factor 1
Monoclonal Antibody for Mouse Serine/arginine-rich splicing factor 1
Monoclonal Antibody for Mouse Serine/arginine-rich splicing factor 1
Monoclonal Antibody for Mouse Serine/arginine-rich splicing factor 1
Monoclonal Antibody for Mouse Serine/arginine-rich splicing factor 1
Monoclonal Antibody for Mouse Serine/arginine-rich splicing factor 1
Protein for Mouse Serine/arginine-rich splicing factor 1
Protein for Mouse Serine/arginine-rich splicing factor 1
Protein for Mouse Serine/arginine-rich splicing factor 1
Protein for Mouse Serine/arginine-rich splicing factor 1
Protein for Mouse Serine/arginine-rich splicing factor 1
R&D Technical Data
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Precision
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Recovery
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Linearity
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Science309:1559-1563(2005)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2)
strain :
C57BL/6J .
tissue :
Bone marrow macrophage .
tissue :
Eye .
tissue :
Medulla oblongata .
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PLoS Biol.7:E1000112-E1000112(2009)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
strain :
C57BL/6J .
3.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
strain :
C57BL/6 .
tissue :
Embryonic brain .
4.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PROTEIN SEQUENCE OF 18-28
tissue :
Brain .
5.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION BY CLK1; CLK2; CLK3 AND CLK4
6.
"ASF/SF2-regulated CaMKIIdelta alternative splicing temporally reprograms excitation-contraction coupling in cardiac muscle."
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,
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Cell120:59-72(2005)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION
7.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199 AND SER-201;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Embryonic fibroblast .
8.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : STRUCTURE BY NMR OF 113-207