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Index > Protein center > TGFA(Gene name) > Human
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  • TGFA (Gene name),
  • Protransforming growth factor alpha (Protein name ),  TGFA_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    TGFA;
    Protein name:
    Protransforming growth factor alpha;
    Alternative:

    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2.
    Function:
    TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
    Subcellular Location:
    Protransforming growth factor alpha Cell membrane Single-pass type I membrane protein
    Protein Attributes:
    Sequence length:
    160
    Sequence:
    50:
    MVPSAGQLAL | FALGIVLAAC | QALENSTSPL | SADPPVAAAV | VSHFNDCPDS | 
    100:
    HTQFCFHGTC | RFLVQEDKPA | CVCHSGYVGA | RCEHADLLAV | VAASQKKQAI | 
    150:
    TALVVVSIVA | LAVLIITCVL | IHCCQVRKHC | EWCRALICRH | EKPSALLKGR | 
    160:
    TACCHSETVV | 
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    UniGene:
    SMR:
    MIM:
    KEGG:
    String:
    Uniprot:
     
    FOR
    ELISA Kit for Human Protransforming growth factor alpha
    Cat.:
    E0123m
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Human Protransforming growth factor alpha
    Cat.:
    E0123h
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Human Protransforming growth factor alpha
    Cat.:
    E0123r
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Human Protransforming growth factor alpha
    MSDS:
    Please sign in first.
    ELISA Kit for Human Protransforming growth factor alpha
    Cat.:
    E0123p
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    CLIA Kit for Human Protransforming growth factor alpha
    Cat.:
    U0123p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Protransforming growth factor alpha
    Cat.:
    U0123h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Protransforming growth factor alpha
    MSDS:
    Please sign in first.
    CLIA Kit for Human Protransforming growth factor alpha
    Cat.:
    U0123m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Protransforming growth factor alpha
    Cat.:
    U0123r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human Protransforming growth factor alpha
    Cat.:
    P0123Rb-m
    Price:
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Protransforming growth factor alpha
    Polyclonal Antibody for Human Protransforming growth factor alpha
    Cat.:
    P0123Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Protransforming growth factor alpha
    Polyclonal Antibody for Human Protransforming growth factor alpha
    Cat.:
    P0123Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human Protransforming growth factor alpha
    Monoclonal Antibody for Human Protransforming growth factor alpha
    Monoclonal Antibody for Human Protransforming growth factor alpha
    Monoclonal Antibody for Human Protransforming growth factor alpha
    Monoclonal Antibody for Human Protransforming growth factor alpha
    Protein for Human Protransforming growth factor alpha
    Protein for Human Protransforming growth factor alpha
    Protein for Human Protransforming growth factor alpha
    Protein for Human Protransforming growth factor alpha
    Protein for Human Protransforming growth factor alpha

    R&D Technical Data
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    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤6.1%

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    Recovery
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    Recovery was determined by spiking various levels of TGFA into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    98

    95-102

    Plasma

    97

    93-104

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TGFA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    95-100%

    96-103%

    94-99%

    94-101%

    EDTA plasma(n=5)

    96-105%

    90-98%

    92-101%

    93-104%

    heparin plasma(n=5)

    92-101%

     

    95-99%

    97-102%

    96-103%

     

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    References
    1. 1.
      "Human transforming growth factor-alpha: precursor structure and expression in E. coli."
      Derynck R. , Roberts A.B. , Winkler M.E. , Chen E.Y. , Goeddel D.V.
      Cell38:287-297(1984) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    2. 2.
      "A novel low molecular weight ribonucleic acid (RNA) related to transforming growth factor alpha messenger RNA."
      Jakowlew S.B. , Kondaiah P. , Dillard P.J. , Sporn M.B. , Roberts A.B.
      Mol. Endocrinol.2:1056-1063(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    3. 3.
      "Human transforming growth factor alpha: sequence analysis of the 4.5-kb and 1.6-kb mRNA species."
      Qian J.F. , Lazar-Wesley E. , Breugnot C. , May E.
      Gene132:291-296(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
      tissue: Colon.
    4. 4.
      "Transforming growth factor-alpha: characterization of the BamHI, RsaI, and TaqI polymorphic regions."
      Qian J.F. , Feingold J. , Stoll C. , May E.
      Am. J. Hum. Genet.53:168-175(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    5. 5.
      "Transforming growth factor-alpha (TGFA): genomic structure, boundary sequences, and mutation analysis in nonsyndromic cleft lip/palate and cleft palate only."
      Machida J. , Yoshiura K. , Funkhauser C.D. , Natsume N. , Kawai T. , Murray J.C.
      Genomics61:237-242(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    6. 6.
      "Human keratinocytes and tumor-derived cell lines express alternatively spliced forms of transforming growth factor-alpha mRNA, encoding precursors lacking carboxyl-terminal valine residues."
      Xu X. , Liao J. , Creek K.E. , Pirisi L.
      Oncogene18:5554-5562(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3; 4 AND 5);TISSUE SPECIFICITY
    7. 7.
      "Cloning of human full-length CDSs in BD Creator(TM) System Donor vector."
      Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
      Submitted (2003-05) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
    8. 8.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      tissue: Thalamus.
    9. 9.
      "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W. , Graves T.A. , Fulton R.S. , Fulton L.A. , Pepin K.H. , Minx P. , Wagner-McPherson C. , Layman D. , Wylie K. , Sekhon M. , Becker M.C. , Fewell G.A. , Delehaunty K.D. , Miner T.L. , Nash W.E. , Kremitzki C. , Oddy L. , Du H. , more...
      Nature434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    10. 10.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    11. 11.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
      tissue: Kidney.
    12. 12.
      "The human transforming growth factor alpha promoter directs transcription initiation from a single site in the absence of a TATA sequence."
      Jakobovits E.B. , Schlokat U. , Vannice J.L. , Derynck R. , Levinson A.D.
      Mol. Cell. Biol.8:5549-5554(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-58
    13. 13.
      "TGFA: exon-intron structure and evaluation as a candidate gene for Alstrom syndrome."
      Collin G.B. , Marshall J.D. , Naggert J.K. , Nishina P.M.
      Clin. Genet.55:61-62(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31
    14. 14.
      "Characterization of disulfide bond position in proteins and sequence analysis of cystine-bridged peptides by tandem mass spectrometry."
      Bean M.F. , Carr S.A.
      Anal. Biochem.201:216-226(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISULFIDE BONDS
    15. 15.
      "Cysteines 153 and 154 of transmembrane transforming growth factor-alpha are palmitoylated and mediate cytoplasmic protein association."
      Shum L. , Turck C.W. , Derynck R.
      J. Biol. Chem.271:28502-28508(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PALMITOYLATION AT CYS-153 AND CYS-154
    16. 16.
      "A role for a PDZ protein in the early secretory pathway for the targeting of proTGF-alpha to the cell surface."
      Fernandez-Larrea J. , Merlos-Suarez A. , Urena J.M. , Baselga J. , Arribas J.
      Mol. Cell3:423-433(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SNTA1 AND SDCBP
    17. 17.
      "Cornichon regulates transport and secretion of TGFalpha-related proteins in metazoan cells."
      Perez Castro C. , Piscopo D. , Nakagawa T. , Derynck R.
      J. Cell Sci.120:2454-2466(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CNIH AND GORASP2
    18. 18.
      "EGF receptor-independent action of TGF-alpha protects Naked2 from AO7-mediated ubiquitylation and proteasomal degradation."
      Ding W. , Li C. , Hu T. , Graves-Deal R. , Fotia A.B. , Weissman A.M. , Coffey R.J.
      Proc. Natl. Acad. Sci. U.S.A.105:13433-13438(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH NKD2
    19. 19.
      "Solution structures of human transforming growth factor alpha derived from 1H NMR data."
      Kline T.P. , Brown F.K. , Brown S.C. , Jeffs P.W. , Kopple K.D. , Mueller L.
      Biochemistry29:7805-7813(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF TGF-ALPHA
    20. 20.
      "The solution structure of human transforming growth factor alpha."
      Harvey T.S. , Wilkinson A.J. , Tappin M.J. , Cooke R.M. , Campbell I.D.
      Eur. J. Biochem.198:555-562(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF TGF-ALPHA
    21. 21.
      "Solution structure of human type-alpha transforming growth factor determined by heteronuclear NMR spectroscopy and refined by energy minimization with restraints."
      Moy F.J. , Li Y.C. , Rauenbuehler P. , Winkler M.E. , Scheraga H.A. , Montelione G.T.
      Biochemistry32:7334-7353(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF TGF-ALPHA
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