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Index > Protein center > TG(Gene name) > Bovine
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  • TG (Gene name),
  • Thyroglobulin (Protein name ),  THYG_BOVIN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Gene name:
    TG;
    Protein name:
    Thyroglobulin(Tg);
    Alternative:

    Organism:
    Bovine (Bos taurus). 
    General Annotation
    Sub Unit:
    Homodimer.
    Function:
    Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3).
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    2769
    Sequence:
    50:
    MALALWVFGL | LDLICLASAN | IFEYQVDAQP | LRPCELQRER | AFLKREDYVP | 
    100:
    QCAEDGSFQT | VQCGKDGASC | WCVDADGREV | PGSRQPGRPA | ACLSFCQLQK | 
    150:
    QQILLSSYIN | STATSYLPQC | QDSGDYSPVQ | CDLRRRQCWC | VDAEGMEVYG | 
    200:
    TRQQGRPARC | PRSCEIRNRR | LLHGVGDRSP | PQCSPDGAFR | PVQCKLVNTT | 
    250:
    DMMIFDLVHS | YSRFPDAFVT | FSSFRSRFPE | VSGYCYCADS | QGRELAETGL | 
    300:
    ELLLDEIYDT | IFAGLDLAST | FAETTLYRIL | QRRFLAVQLV | ISGRFRCPTK | 
    350:
    CEVERFAATS | FRHPYVPSCH | PDGEYQAAQC | QQGGPCWCVD | SRGQEIPGTR | 
    400:
    QRGEPPSCAE | DQSCPSERRR | AFSRLRFGPS | GYFSRRSLLL | APEEGPVSQR | 
    450:
    FARFTASCPP | SIKELFLDSG | IFQPMLQGRD | TRFVAPESLK | EAIRGLFPSR | 
    500:
    ELARLALQFT | TNAKRLQQNL | FGGRFLVKVG | QFNLSGALGT | RGTFNFSHFF | 
    550:
    QQLGLPGFQD | GRALADLAKP | LSVGLNSNPA | SEAPKASKID | VALRKPVVGS | 
    600:
    FGFEVNLQEN | QNALQFLSSF | LELPEFLLFL | QHAISVPEDI | ARDLGDVMEM | 
    650:
    VFSSQGCGQA | PGSLFVPACT | AEGSYEEVQC | FAGDCWCVDA | QGRELAGSRV | 
    700:
    RGGRPRCPTE | CEKQRARMQS | LLGSQPAGSS | LFVPACTSKG | NFLPVQCFNS | 
    750:
    ECYCVDTEGQ | PIPGTRSALG | EPKKCPSPCQ | LQAERAFLGT | VRTLVSNPST | 
    800:
    LPALSSIYIP | QCSASGQWSP | VQCDGPPEQA | FEWYERWEAQ | NSAGQALTPA | 
    850:
    ELLMKIMSYR | EAASRNFRLF | IQNLYEAGQQ | GIFPGLARYS | SFQDVPVSVL | 
    900:
    EGNQTQPGGN | VFLEPYLFWQ | ILNGQLDRYP | GPYSDFSAPL | AHFDLRSCWC | 
    950:
    VDEAGQKLEG | TRNEPNKVPA | CPGSCEEVKL | RVLQFIREAE | EIVTYSNSSR | 
    1000:
    FPLGESFLAA | KGIRLTDEEL | AFPPLSPSRE | TFLEKFLSGS | DYAIRLAAQS | 
    1050:
    TFDFYQRRLV | TLAESPRAPS | PVWSSAYLPQ | CDAFGGWEPV | QCHAATGHCW | 
    1100:
    CVDGKGEYVP | TSLTARSRQI | PQCPTSCERL | RASGLLSSWK | QAGVQAEPSP | 
    1150:
    KDLFIPTCLE | TGEFARLQAS | EAGTWCVDPA | SGEGVPPGTN | SSAQCPSLCE | 
    1200:
    VLQSGVPSRR | TSPGYSPACR | AEDGGFSPVQ | CDPAQGSCWC | VLGSGEEVPG | 
    1250:
    TRVAGSQPAC | ESPQCPLPFS | VADVAGGAIL | CERASGLGAA | AGQRCQLRCS | 
    1300:
    QGYRSAFPPE | PLLCSVQRRR | WESRPPQPRA | CQRPQFWQTL | QTQAQFQLLL | 
    1350:
    PLGKVCSADY | SGLLLAFQVF | LLDELTARGF | CQIQVKTAGT | PVSIPVCDDS | 
    1400:
    SVKVECLSRE | RLGVNITWKL | QLVDAPPASL | PDLQDVEEAL | AGKYLAGRFA | 
    1450:
    DLIQSGTFQL | HLDSKTFSAD | TSIRFLQGDR | FGTSPRTQFG | CLEGFGRVVA | 
    1500:
    ASDASQDALG | CVKCPEGSYF | QDEQCIPCPA | GFYQEQAGSL | ACVPCPEGRT | 
    1550:
    TVYAGAFSQT | HCVTDCQKNE | VGLQCDQDSQ | YRASQRDRTS | GKAFCVDGEG | 
    1600:
    RRLPWTEAEA | PLVDAQCLVM | RKFEKLPESK | VIFSADVAVM | VRSEVPGSES | 
    1650:
    SLMQCLADCA | LDEACGFLTV | STAGSEVSCD | FYAWASDSIA | CTTSGRSEDA | 
    1700:
    LGTSQATSFG | SLQCQVKVRS | REGDPLAVYL | KKGQEFTITG | QKRFEQTGFQ | 
    1750:
    SALSGMYSPV | TFSASGASLA | EVHLFCLLAC | DHDSCCDGFI | LVQVQGGPLL | 
    1800:
    CGLLSSPDVL | LCHVRDWRDP | AEAQANASCP | GVTYDQDSRQ | VTLRLGGQEI | 
    1850:
    RGLTPLEGTQ | DTLTSFQQVY | LWKDSDMGSR | SESMGCRRDT | EPRPASPSET | 
    1900:
    DLTTGLFSPV | DLIQVIVDGN | VSLPSQQHWL | FKHLFSLQQA | NLWCLSRCAG | 
    1950:
    EPSFCQLAEV | TDSEPLYFTC | TLYPEAQVCD | DILESSPKGC | RLILPRRPSA | 
    2000:
    LYRKKVVLQD | RVKNFYNRLP | FQKLTGISIR | NKVPMSDKSI | SSGFFECERL | 
    2050:
    CDMDPCCTGF | GFLNVSQLKG | GEVTCLTLNS | LGLQTCSEEY | GGVWRILDCG | 
    2100:
    SPDTEVRTYP | FGWYQKPVSP | SDAPSFCPSV | ALPALTENVA | LDSWQSLALS | 
    2150:
    SVIVDPSIRN | FDVAHISTAA | VGNFSAARDR | CLWECSRHQD | CLVTTLQTQP | 
    2200:
    GAVRCMFYAD | TQSCTHSLQA | QNCRLLLHEE | ATYIYRKPNI | PLPGFGTSSP | 
    2250:
    SVPIATHGQL | LGRSQAIQVG | TSWKPVDQFL | GVPYAAPPLG | EKRFRAPEHL | 
    2300:
    NWTGSWEATK | PRARCWQPGI | RTPTPPGVSE | DCLYLNVFVP | QNMAPNASVL | 
    2350:
    VFFHNAAEGK | GSGDRPAVDG | SFLAAVGNLI | VVTASYRTGI | FGFLSSGSSE | 
    2400:
    LSGNWGLLDQ | VVALTWVQTH | IQAFGGDPRR | VTLAADRGGA | DIASIHLVTT | 
    2450:
    RAANSRLFRR | AVLMGGSALS | PAAVIRPERA | RQQAAALAKE | VGCPSSSVQE | 
    2500:
    MVSCLRQEPA | RILNDAQTKL | LAVSGPFHYW | GPVVDGQYLR | ETPARVLQRA | 
    2550:
    PRVKVDLLIG | SSQDDGLINR | AKAVKQFEES | QGRTSSKTAF | YQALQNSLGG | 
    2600:
    EAADAGVQAA | ATWYYSLEHD | SDDYASFSRA | LEQATRDYFI | ICPVIDMASH | 
    2650:
    WARTVRGNVF | MYHAPESYSH | SSLELLTDVL | YAFGLPFYPA | YEGQFTLEEK | 
    2700:
    SLSLKIMQYF | SNFIRSGNPN | YPHEFSRRAP | EFAAPWPDFV | PRDGAESYKE | 
    2750:
    LSVLLPNRQG | LKKADCSFWS | KYIQSLKASA | DETKDGPSAD | SEEEDQPAGS | 
    2769:
    GLTEDLLGLP | ELASKTYSK
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    UniGene:
    Pfam:
    String:
    Uniprot:
     
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    ELISA Kit for Bovine Tg
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    ELISA Kit for Bovine Tg
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    CLIA Kit for Bovine Tg
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    CLIA Kit for Bovine Tg
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    CLIA Kit for Bovine Tg
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    Polyclonal Antibody for Bovine Tg
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    P0355Rb-h
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    Polyclonal Antibody for Bovine Tg
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    Polyclonal Antibody for Bovine Tg
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    Packing:
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    Polyclonal Antibody for Bovine Tg
    Polyclonal Antibody for Bovine Tg
    Monoclonal Antibody for Bovine Tg
    Monoclonal Antibody for Bovine Tg
    Monoclonal Antibody for Bovine Tg
    Monoclonal Antibody for Bovine Tg
    Monoclonal Antibody for Bovine Tg
    Protein for Bovine Tg
    Protein for Bovine Tg
    Protein for Bovine Tg
    Protein for Bovine Tg
    Protein for Bovine Tg

    R&D Technical Data
    s
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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    Saliva(n=3)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    References
    1. 1.
      "Primary structure of bovine thyroglobulin deduced from the sequence of its 8,431-base complementary DNA."
      Mercken L. , Simons M.-J. , Swillens S. , Massaer M. , Vassart G.
      Nature316:647-651(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    2. 2.
      "Presence of hormonogenic and repetitive domains in the first 930 amino acids of bovine thyroglobulin as deduced from the cDNA sequence."
      Mercken L. , Simons M.-J. , de Martynhoff G. , Swillens S. , Vassart G.
      Eur. J. Biochem.147:59-64(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-930
    3. 3.
      "Structural organization of the bovine thyroglobulin gene and of its 5'-flanking region."
      de Martynhoff G. , Pohl V. , Mercken L. , van Ommen G.-J. , Vassart G.
      Eur. J. Biochem.164:591-599(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22
    4. 4.
      "Structural organization of the 5' region of the thyroglobulin gene. Evidence for intron loss and 'exonization' during evolution."
      Parma J. , Christophe D. , Pohl V. , Vassart G.
      J. Mol. Biol.196:769-779(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1002-1209
    5. 5.
      "Thyroglobulin glycosylation: location and nature of the N-linked oligosaccharide units in bovine thyroglobulin."
      Rawitch A.B. , Pollock H.G. , Yang S.X.
      Arch. Biochem. Biophys.300:271-279(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT ASN-110; ASN-483; ASN-495; ASN-853; ASN-947; ASN-1140; ASN-1365; ASN-1776; ASN-1870; ASN-2014; ASN-2123; ASN-2251 AND ASN-2296;ABSENCE OF GLYCOSYLATION AT ASN-198;STRUCTURE OF CARBOHYDRATE
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